Alkane hydroxylase homologues in Gram-positive strains

Summary We isolated Gram‐positive alkane‐degraders from soil and a tricking‐bed reactor, and show using polymerase chain reaction (PCR) with degenerate alkane hydroxylase primers and Southern blots that most Rhodococcus isolates contain three to five quite divergent homologues of the Pseudomonas put...

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Bibliographic Details
Published in:	Environmental microbiology Vol. 4; no. 11; pp. 676 - 682
Main Authors:	Van Beilen, Jan B., Smits, Theo H. M., Whyte, Lyle G., Schorcht, Susanne, Röthlisberger, Martina, Plaggemeier, Thorsten, Engesser, Karl-Heinrich, Witholt, Bernard
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Science Ltd 01-11-2002
Subjects:	Alkanes - metabolism Blotting, Southern Cytochrome P-450 CYP4A Cytochrome P-450 Enzyme System - metabolism Gram-Positive Bacteria - classification Gram-Positive Bacteria - enzymology Mixed Function Oxygenases - metabolism Phylogeny Polymerase Chain Reaction Soil Microbiology
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Summary:	Summary We isolated Gram‐positive alkane‐degraders from soil and a tricking‐bed reactor, and show using polymerase chain reaction (PCR) with degenerate alkane hydroxylase primers and Southern blots that most Rhodococcus isolates contain three to five quite divergent homologues of the Pseudomonas putida GPo1 alkB gene. Two Mycobacterium isolates each contain one homologue, however there is no evidence for the presence of alkB homologues in the remaining strains.
Bibliography:	ArticleID:EMI355 ark:/67375/WNG-1RD5SC3X-V istex:4BF3CFB186D45E4CCD0448D5ABF908B56AEB8369 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1462-2912 1462-2920
DOI:	10.1046/j.1462-2920.2002.00355.x