Effect of amiodarone on thermotolerance and Hsp104p synthesis in the yeast Saccharomyces cerevisiae

Amiodarone (AMD) is known to induce a transient increase in cytosolic Ca 2+ level in cells of the yeast Saccharomyces cerevisiae . In the present study the effect of AMD on the thermotolerance and Hsp104p synthesis of the yeast was studied. AMD induced Hsp104p synthesis and increased survival of the...

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Published in:	Biochemistry (Moscow) Vol. 77; no. 1; pp. 78 - 86
Main Authors:	Fedoseeva, I. V., Pjatricas, D. V., Varakina, N. N., Rusaleva, T. M., Stepanov, A. V., Rikhvanov, E. G., Borovskii, G. B., Voinikov, V. K.
Format:	Journal Article
Language:	English
Published:	Dordrecht SP MAIK Nauka/Interperiodica 2012 Springer Springer Nature B.V
Subjects:	Amiodarone - chemistry Amiodarone - pharmacology Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Calcium - metabolism Chemical synthesis Enzyme Inhibitors - pharmacology Gene Expression Regulation, Fungal - drug effects Heat-Shock Proteins - metabolism Life Sciences Microbiology Phosphorylation Rhodamines - chemistry Saccharomyces cerevisiae Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - metabolism Temperature Yeast Yeasts amiodarone thermotolerance Hsp104p mitochondrion
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Summary:	Amiodarone (AMD) is known to induce a transient increase in cytosolic Ca 2+ level in cells of the yeast Saccharomyces cerevisiae . In the present study the effect of AMD on the thermotolerance and Hsp104p synthesis of the yeast was studied. AMD induced Hsp104p synthesis and increased survival of the yeast after a severe heat shock (50°C). The development of thermotolerance to a considerable extent depended on the presence of Hsp104p. The same effect was achieved by treatment with the classical uncoupler CCCP, which is also known to increase the cytosolic Ca 2+ level. It is supposed that the change in intracellular Ca 2+ concentration plays an important role in activation of the HSP104 gene expression and in increasing the thermotolerance of the yeast. The possible link between mitochondrial activity and calcium homeostasis is discussed.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-2979 1608-3040
DOI:	10.1134/S0006297912010099