Search Results - "Pizzano, Brianna L.M."

A thermostable Cas12b from Brevibacillus leverages one-pot discrimination of SARS-CoV-2 variants of concern by Nguyen, Long T., Macaluso, Nicolas C., Pizzano, Brianna L.M., Cash, Melanie N., Spacek, Jan, Karasek, Jan, Miller, Megan R., Lednicky, John A., Dinglasan, Rhoel R., Salemi, Marco, Jain, Piyush K.

“…Current SARS-CoV-2 detection platforms lack the ability to differentiate among variants of concern (VOCs) in an efficient manner. CRISPR/Cas (Clustered…”
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Harnessing noncanonical crRNAs to improve functionality of Cas12a orthologs by Nguyen, Long T., Macaluso, Nicolas C., Rakestraw, Noah R., Carman, Dylan R., Pizzano, Brianna L.M., Hautamaki, Raymond C., Rananaware, Santosh R., Roberts, Isabel E., Jain, Piyush K.

“…There is a broad diversity among Cas12a endonucleases that possess nucleic acid detection and gene-editing capabilities, but few are studied extensively. Here,…”
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CRISPR-ENHANCE: An enhanced nucleic acid detection platform using Cas12a by Nguyen, Long T., Gurijala, Jeevan, Rananaware, Santosh R., Pizzano, Brianna L.M., Stone, Brandon T., Jain, Piyush K.

“…Rapid detection of nucleic acids is essential for clinical diagnosis of a wide range of infectious and non-infectious diseases. CRISPR-based diagnostic…”
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Clinical validation of engineered CRISPR/Cas12a for rapid SARS-CoV-2 detection by Nguyen, Long T., Rananaware, Santosh R., Pizzano, Brianna L. M., Stone, Brandon T., Jain, Piyush K.

“…Background The coronavirus disease (COVID-19) caused by SARS-CoV-2 has swept through the globe at an unprecedented rate. CRISPR-based detection technologies…”
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Engineering highly thermostable Cas12b via de novo structural analyses for one-pot detection of nucleic acids by Nguyen, Long T., Rananaware, Santosh R., Yang, Lilia G., Macaluso, Nicolas C., Ocana-Ortiz, Julio E., Meister, Katelyn S., Pizzano, Brianna L.M., Sandoval, Luke Samuel W., Hautamaki, Raymond C., Fang, Zoe R., Joseph, Sara M., Shoemaker, Grace M., Carman, Dylan R., Chang, Liwei, Rakestraw, Noah R., Zachary, Jon F., Guerra, Sebastian, Perez, Alberto, Jain, Piyush K.

“…CRISPR-Cas-based diagnostics have the potential to elevate nucleic acid detection. CRISPR-Cas systems can be combined with a pre-amplification step in a…”
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