Characterization and location of secretory phospholipase A2 groups IIE, V, and X in the rat brain

Secretory phospholipases A2 (sPLA2) form a diverse family of enzymes involved in a variety of physiologic and pathologic processes. Common among all sPLA2 is the ability to cleave the second position of phospholipids, thereby releasing fatty acid and a lysophospholipid. Several sPLA2 have been clone...

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Bibliographic Details
Published in:	Journal of neuroscience research Vol. 83; no. 5; pp. 874 - 882
Main Authors:	Kolko, Miriam, Christoffersen, Nanna R., Barreiro, Sebastian G., Miller, Martin L., Pizza, Andrew J., Bazan, Nicolas G.
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-04-2006
Subjects:	Animals Brain - physiology Cells, Cultured Embryo, Mammalian Group II Phospholipases A2 Immunohistochemistry In Situ Hybridization lipid signaling Medicin och hälsovetenskap neuronal signaling Neurons - physiology Phospholipases A - genetics Phospholipases A - metabolism Phospholipases A2 Rats Reverse Transcriptase Polymerase Chain Reaction seizure
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Summary:	Secretory phospholipases A2 (sPLA2) form a diverse family of enzymes involved in a variety of physiologic and pathologic processes. Common among all sPLA2 is the ability to cleave the second position of phospholipids, thereby releasing fatty acid and a lysophospholipid. Several sPLA2 have been cloned and characterized in various tissues and receptors have been identified. In the nervous system, sPLA2 groups IB, IIA, IIE, IIF, V, and XII have been identified, and binding sites for sPLA2 have been found. Here, we report sPLA2‐IIE and sPLA2‐X in rat brain as well as in neurons in primary culture. We furthermore confirm the presence of sPLA2‐V in rat brain and demonstrate the presence of sPLA2‐V in primary neuronal cultures. The distribution of sPLA2‐IIE, V, and ‐X seems to be mainly neuronal, with the highest abundance occurring in the cerebral cortex and hippocampus. We also find that sPLA2‐IIE, ‐V, and ‐X are differentially induced by kainic acid. This study supports the concept that sPLA2 heterogeneity in brain is functionally relevant and responsive to seizures. © 2006 Wiley‐Liss, Inc.
Bibliography:	Gerda og Aage Haensch's Fond United States Public Health Service, National Institute of Neurologic Diseases and Stroke, National Institutes of Health - No. NS23002; No. NS46741 Hørslev-Fonden Torben og Alice Frimodts Fond Familien Hede Nielsens Fond Else og Mogens Wedell-Wedellborgs Fond ark:/67375/WNG-XBVVQG3V-M Dagmar Marshalls Fond Beckett-Fonden ArticleID:JNR20773 istex:5AA690D74C39186E1004DF152F9B44276B12EE7B Else og Aage Groenbeck-Olsens Legat Gangstedfonden ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0360-4012 1097-4547
DOI:	10.1002/jnr.20773