Platelet-rich plasma induces annulus fibrosus cell proliferation and matrix production

Purpose Platelet-rich plasma (PRP) contains growth factors and creates a 3D structure upon clotting; PRP or platelet lysate (PL) might be considered for annulus fibrosus (AF) repair. Methods Bovine AF cells were cultured with 25 % PRP, 50 % PRP, 25 % PL, 50 % PL, or 10 % FBS. After 2 and 4 days, DNA...

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Bibliographic Details
Published in:	European spine journal Vol. 23; no. 4; pp. 745 - 753
Main Authors:	Pirvu, T. N., Schroeder, J. E., Peroglio, M., Verrier, S., Kaplan, L., Richards, R. G., Alini, M., Grad, S.
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 01-04-2014 Springer Nature B.V
Subjects:	Animals Biomarkers - metabolism Cattle Cell Proliferation - physiology Cells, Cultured Collagen - metabolism Extracellular Matrix - physiology Feasibility Studies Glycosaminoglycans - metabolism Guided Tissue Regeneration - methods Intervertebral Disc - metabolism Intervertebral Disc - physiology Medicine Medicine & Public Health Neurosurgery Organ Culture Techniques Original Original Article Platelet-Rich Plasma Regeneration Surgical Orthopedics Annulus fibrosus Platelet lysate Proteoglycans Intervertebral disc Platelet-rich plasma
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Summary:	Purpose Platelet-rich plasma (PRP) contains growth factors and creates a 3D structure upon clotting; PRP or platelet lysate (PL) might be considered for annulus fibrosus (AF) repair. Methods Bovine AF cells were cultured with 25 % PRP, 50 % PRP, 25 % PL, 50 % PL, or 10 % FBS. After 2 and 4 days, DNA, glycosaminoglycan (GAG), and mRNA levels were analyzed. Histology was performed after injection of PRP into an AF defect in a whole disc ex vivo. Results By day 4, significant increases in DNA content were observed in all treatment groups. All groups also showed elevated GAG synthesis, with highest amounts at 50 % PL. Collagen I and II expression was similar between groups; aggrecan, decorin, and versican expression was highest at 25 % PL. Injection of PRP into the AF defect resulted in an increased matrix synthesis. Conclusions Platelet-rich preparations increased the matrix production and cell number and may therefore be considered to promote AF repair.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23 ObjectType-Feature-1
ISSN:	0940-6719 1432-0932
DOI:	10.1007/s00586-014-3198-x