Search Results - "Pirone, T.P"

A Specific Interaction between Coat Protein and Helper Component Correlates with Aphid Transmission of a Potyvirus by Blanc, Stéphane, López-Moya, Juan-José, Wang, Renyuan, García-Lampasona, Sandra, Thornbury, David W., Pirone, Thomas P.

“…Specific binding between the coat protein (CP) and the helper component (HC) of the tobacco vein mottling potyvirus (TVMV) was characterized using a protein…”
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Site-directed mutations in the potyvirus HC-Pro gene affect helper component activity, virus accumulation, and symptom expression in infected tobacco plants by Atreya, C D, Atreya, P L, Thornbury, D W, Pirone, T P

“…Helper component (HC-Pro) is a virus-encoded nonstructural protein required for aphid transmission of potyviruses. In the tobacco vein mottling virus (TVMV)…”
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A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus by Atreya, C D, Raccah, B, Pirone, T P

“…A nonaphid transmissible (NAT) variant of tobacco vein mottling virus (TVMV) was used to test the hypothesis that the viral coat protein (CP) plays a role in…”
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Helper-dependent vector transmission of plant viruses by Pirone, T.P. (University of Kentucky, Lexington, KY.), Blanc, S

“…A variety of noncirculatively transmitted viruses have evolved a vector transmission strategy that involves, in addition to virions, virus-encoded proteins…”
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Selection of deletion mutants by polymerase chain reaction by Atreya, C D, Atreya, P L, Pirone, T P

“…Polymerase chain reaction (PCR) based DNA amplification has replaced many time-consuming protocols in molecular biology. Here we describe a simple strategy to…”
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Mutations in the potyvirus helper component protein: effects on interactions with virions and aphid stylets by Blanc, S, Ammar, ED, Garcia-Lampasona, S, Dolja, VV, Llave, C, Baker, J, Pirone, TP

“…S Blanc, ED Ammar, S Garcia-Lampasona, VV Dolja, C Llave, J Baker and TP Pirone Department of Plant Pathology, University of Kentucky, Lexington 40546, USA…”
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Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility by Atreya, C.D, Pirone, T.P

“…We have previously provided evidence that amino acid substitutions within the N-terminal portion of the helper component-proteinase (HC-Pro) from tobacco vein…”
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Spontaneous mutagenesis of a plant potyvirus genome after insertion of a foreign gene by Dolja, V.V, Herndon, K.L, Pirone, T.P, Carrington, J.C

“…The RNA genome of tobacco etch potyvirus (TEV) was engineered to express bacterial beta-glucuronidase (GUS) fused to the virus helper component proteinase…”
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Purification and characterization of turnip mosaic virus helper component protein by Wang, R.Y, Pirone, T.P

“…The helper component (HC) protein of turnip mosaic virus (TuMV) was concentrated by differential centrifugation followed by ammonium sulfate precipitation. The…”
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Nomenclature and concepts of pathogenicity and virulence by Shaner, G. (Purdue University, West Lafayette, IN), Stromberg, E.L, Lacy, G.H, Barker, K.R, Pirone, T.P

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Mineral oil interferes with retention of tobacco etch potyvirus in the stylets of Myzus persicae by Wang, R.Y. (University of Kentucky, Lexington, KY.), Pirone, T.P

“…Light microscopic autoradiography was used to study the mode of action of mineral oil in reducing transmission of tobacco etch potyvirus (TEV) by Myzus…”
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Histidine-tagging and purification of tobacco etch potyvirus helper component protein by Blanc, Stéphane, V. Dolja, Valerian, Llave, Cesar, P. Pirone, Thomas

“…The coding sequence for a series of six histidines (his-tag) was inserted near the 5′ terminus of the helper component (HC) coding region of tobacco etch…”
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Comparative sequence of the helper component (HC) region of potato virus Y and a HC-defective strain, potato virus C by Thornbury, D W, Patterson, C A, Dessens, J T, Pirone, T P

“…Potato virus C (PVC), a non-aphid transmissible strain of potato virus Y (PVY), was found to code for a protein (PVC-HC) which is similar in molecular weight…”
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Construction and analysis of infectious transcripts from a resistance-breaking strain of tobacco vein mottling potyvirus by Nicolas, O, Pirone, T.P, Hellmann, G.M

“…The Burley tobacco (Nicotiana tabacum) cultivar TN 86 is "resistant" to most strains of tobacco vein mottling potyvirus (TVMV), the virus being restricted to…”
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Association with virions and coat protein of tobacco vein mottling potyvirus with cylindrical inclusions in tobacco cells by Ammar, E.D. (University of Kentucky, Lexington), Rodriguez-Cerezo, E, Shaw, J.G, Pirone, T.P

“…The course of infection with tobacco vein mottling potyvirus (TVMV) in tobacco protoplasts and leaves was studied by electron microscopy with immunogold…”
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Nature of resistance of a tobacco cultivar to tobacco vein mottling virus by Gibb, K.S, Hellmann, G.M, Pirone, T.P

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Amino acid substitutions in the coat protein results in loss of insect transmissibility of a plant virus by Atreya, P.L. (University of Kentucky, Lexington, KY), Atreya, C.D, Pirone, T.P

“…Amino acids near the N terminus of the coat protein of tobacco vein mottling virus were deleted or altered by site-directed mutagenesis to determine the effect…”
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Analysis of spatial patterns of virus-diseased tobacco plants by Madden, L.V, Pirone, T.P, Raccah, B

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Efficiency and selectivity of the helper-component-mediated aphid transmission of purified potyviruses [Myzus persicae, Plant Pathology] by Pirone, Thomas P.

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Role of virion and helper component in regulating aphid transmission of tobacco etch virus [Myzus persicae as vector, Nicotiana tabacum] by Pirone, Thomas P.

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