Search Results - "Pippig, Diana"

Monodisperse measurement of the biotin-streptavidin interaction strength in a well-defined pulling geometry by Sedlak, Steffen M, Bauer, Magnus S, Kluger, Carleen, Schendel, Leonard C, Milles, Lukas F, Pippig, Diana A, Gaub, Hermann E

“…The widely used interaction of the homotetramer streptavidin with the small molecule biotin has been intensively studied by force spectroscopy and has become a…”
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Direction Matters: Monovalent Streptavidin/Biotin Complex under Load by Sedlak, Steffen M, Schendel, Leonard C, Melo, Marcelo C. R, Pippig, Diana A, Luthey-Schulten, Zaida, Gaub, Hermann E, Bernardi, Rafael C

“…Novel site-specific attachment strategies combined with improvements of computational resources enable new insights into the mechanics of the monovalent…”
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Different Vinculin Binding Sites Use the Same Mechanism to Regulate Directional Force Transduction by Kluger, Carleen, Braun, Lukas, Sedlak, Steffen M., Pippig, Diana A., Bauer, Magnus S., Miller, Ken, Milles, Lukas F., Gaub, Hermann E., Vogel, Viola

“…Vinculin is a universal adaptor protein that transiently reinforces the mechanical stability of adhesion complexes. It stabilizes mechanical connections that…”
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Force sensing by the vascular protein von Willebrand factor is tuned by a strong intermonomer interaction by Müller, Jochen P., Mielke, Salomé, Löf, Achim, Obser, Tobias, Beer, Christof, Bruetzel, Linda K., Pippig, Diana A., Vanderlinden, Willem, Lipfert, Jan, Schneppenheim, Reinhard, Benoit, Martin

“…The large plasma glycoprotein von Willebrand factor (VWF) senses hydrodynamic forces in the bloodstream and responds to elevated forces with abrupt elongation,…”
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Monovalent Strep-Tactin for strong and site-specific tethering in nanospectroscopy by Baumann, Fabian, Bauer, Magnus S., Milles, Lukas F., Alexandrovich, Alexander, Gaub, Hermann E., Pippig, Diana A.

“…Strep -Tactin, an engineered form of streptavidin, binds avidly to the genetically encoded peptide Strep -tag II in a manner comparable to streptavidin binding…”
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From genes to protein mechanics on a chip by Otten, Marcus, Ott, Wolfgang, Jobst, Markus A, Milles, Lukas F, Verdorfer, Tobias, Pippig, Diana A, Nash, Michael A, Gaub, Hermann E

“…A microfluidic chip is used to construct a microarray of proteins, each labeled with a dockerin tag, for high-throughput single-molecule force spectroscopy…”
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Protein–DNA Chimeras for Nano Assembly by Pippig, Diana A, Baumann, Fabian, Strackharn, Mathias, Aschenbrenner, Daniela, Gaub, Hermann E

“…In synthetic biology, “understanding by building” requires exquisite control of the molecular constituents and their spatial organization. Site-specific…”
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regulatory domain of the RIG-I family ATPase LGP2 senses double-stranded RNA by Pippig, Diana A, Hellmuth, Johannes C, Cui, Sheng, Kirchhofer, Axel, Lammens, Katja, Lammens, Alfred, Schmidt, Andreas, Rothenfusser, Simon, Hopfner, Karl-Peter

“…RIG-I and MDA5 sense cytoplasmic viral RNA and set-off a signal transduction cascade, leading to antiviral innate immune response. The third RIG-I-like…”
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A force-based, parallel assay for the quantification of protein-DNA interactions by Limmer, Katja, Pippig, Diana A, Aschenbrenner, Daniela, Gaub, Hermann E

“…Analysis of transcription factor binding to DNA sequences is of utmost importance to understand the intricate regulatory mechanisms that underlie gene…”
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Parallel force assay for protein-protein interactions by Aschenbrenner, Daniela, Pippig, Diana A, Klamecka, Kamila, Limmer, Katja, Leonhardt, Heinrich, Gaub, Hermann E

“…Quantitative proteome research is greatly promoted by high-resolution parallel format assays. A characterization of protein complexes based on binding forces…”
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Tip localization of an atomic force microscope in transmission microscopy with nanoscale precision by Baumann, Fabian, Heucke, Stephan F, Pippig, Diana A, Gaub, Hermann E

“…Since the atomic force microscope (AFM) has evolved into a general purpose platform for mechanical experiments at the nanoscale, the need for a simple and…”
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Nanoscale Arrangement of Proteins by Single-Molecule Cut-and-Paste by Strackharn, Mathias, Pippig, Diana A, Meyer, Philipp, Stahl, Stefan W, Gaub, Hermann E

“…Protein-based nanostructures are key to the organization of life and it is their precise arrangement, which determines their specific functions. A…”
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C-5 Propynyl Modifications Enhance the Mechanical Stability of DNA by Aschenbrenner, Daniela, Baumann, Fabian, Milles, Lukas F., Pippig, Diana A., Gaub, Hermann E.

“…Increased thermal or mechanical stability of DNA duplexes is desired for many applications in nanotechnology or ‐medicine where DNA is used as a programmable…”
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A mutation of human cytochrome c enhances the intrinsic apoptotic pathway but causes only thrombocytopenia by da Silva Tatley, Fernanda M, Weeks, Robert J, Wilbanks, Sigurd M, Fagerlund, Robert D, Smith, Mark P, Davies, Stefan M K, Capron, Claude, Ledgerwood, Elizabeth C, Fichelson, Serge, Holyoake, Andrew J, Pippig, Diana A, Morison, Ian M, Cheesman, Emma J, Ludgate, Mathew W, Coker, Melanie S A, Bockett, Nicholas A, Cheong, Pak Leng, Hughes, Gillian, Lo, Alexandra, Cramer Bordé, Elisabeth M

“…We report the first identified mutation in the gene encoding human cytochrome c (CYCS). Glycine 41, invariant throughout eukaryotes, is substituted by serine…”
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Inside Back Cover: C-5 Propynyl Modifications Enhance the Mechanical Stability of DNA (ChemPhysChem 10/2015) by Aschenbrenner, Daniela, Baumann, Fabian, Milles, Lukas F., Pippig, Diana A., Gaub, Hermann E.

“…The increase of mechanical stability of DNA that is due to the integration of propynyl bases is determined by using two complementary force spectroscopy…”
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Increasing evidence of mechanical force as a functional regulator in smooth muscle myosin light chain kinase by Baumann, Fabian, Bauer, Magnus Sebastian, Rees, Martin, Alexandrovich, Alexander, Gautel, Mathias, Pippig, Diana Angela, Gaub, Hermann Eduard

“…Mechanosensitive proteins are key players in cytoskeletal remodeling, muscle contraction, cell migration and differentiation processes. Smooth muscle myosin…”
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HTHP: A Novel Class of Hexameric, Tyrosine-coordinated Heme Proteins by Jeoung, Jae-Hun, Pippig, Diana A., Martins, Berta M., Wagener, Nadine, Dobbek, Holger

“…We have cloned, expressed, isolated and characterized a hexameric tyrosine-coordinated heme protein (HTHP) from the marine bacterium Silicibacter pomeroyi…”
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Structural and mechanistic insights into mechanoactivation of focal adhesion kinase by Bauer, Magnus Sebastian, Baumann, Fabian, Daday, Csaba, Redondo, Pilar, Durner, Ellis, Jobst, Markus Andreas, Milles, Lukas Frederik, Mercadante, Davide, Pippig, Diana Angela, Gaub, Hermann Eduard, Gräter, Frauke, Lietha, Daniel

“…Focal adhesion kinase (FAK) is a key signaling molecule regulating cell adhesion, migration, and survival. FAK localizes into focal adhesion complexes formed…”
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Molecular Tools for Advanced Single-Molecule Studies by Baumann, Fabian, Sebastian Bauer, Magnus, Frederik Milles, Lukas, Eduard Gaub, Hermann, Angela Pippig, Diana

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Force Sensing by the Vascular Protein Von Willebrand Factor is Tuned by a Strong Intermonomer Interaction by Mueller, Jochen P., Mielke, Salomé, Löf, Achim, Obser, Tobias, Beer, Christof, Pippig, Diana A., Vanderlinden, Willem, Schneppenheim, Reinhard, Benoit, Martin

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