Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma

Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma

Canine cutaneous squamous cell carcinoma (cSCC) is the most common skin cancer in dogs, and, due to its low metastatic rate, local treatments, such as electrochemotherapy (ECT), promote disease control or even complete remission (CR). This study aimed to evaluate the gene and protein expression of B...

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Bibliographic Details
Published in:Scientific reports Vol. 9; no. 1; pp. 15819 - 10
Main Authors: Dos Anjos, Denner S., Bueno, Cynthia, Magalhães, Larissa F., Magalhães, Georgia M., Mattos-Junior, Ewaldo, Pinto, Marcela M. R., De Nardi, Andrigo B., Brunner, Carlos H. M., Leis-Filho, Antonio F., Calazans, Sabryna G., Fonseca-Alves, Carlos E.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 01-11-2019
Nature Publishing Group
Subjects:
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631/67/1813/1352
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Animals
Apoptosis
Bax protein
Bcl-2 protein
Bcl-x protein
Carcinoma, Squamous Cell - pathology
Carcinoma, Squamous Cell - therapy
Carcinoma, Squamous Cell - veterinary
Cell Proliferation
Chemotherapy
Disease control
Dog Diseases - therapy
Dogs
Electrochemotherapy
Humanities and Social Sciences
Humans
Medical prognosis
Metastases
multidisciplinary
Protein expression
Proteins
Remission
Science
Science (multidisciplinary)
Skin cancer
Skin Neoplasms - pathology
Skin Neoplasms - therapy
Skin Neoplasms - veterinary
Squamous cell carcinoma
Treatment Outcome
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Summary:Canine cutaneous squamous cell carcinoma (cSCC) is the most common skin cancer in dogs, and, due to its low metastatic rate, local treatments, such as electrochemotherapy (ECT), promote disease control or even complete remission (CR). This study aimed to evaluate the gene and protein expression of Bcl-2 and Bcl-2 associated X protein (BAX), the proliferative index and clinical parameters in dogs with cSCC subjected to ECT. A prospective nonrandomized clinical study was performed using dogs with naturally occurring cSCC that was treated with ECT. Eighteen lesions from 11 dogs were selected. The tumor size at day 0 (D0) had no impact on survival or prognosis (P > 0.05). Tumor samples had a lower proliferative index after ECT (D21) than before ECT (P = 0.031). The survival of subjects with Ki67 values lower and higher than the Ki67 median value were not significantly different (P > 0.05). Regarding apoptotic markers, there were no significant differences in the gene and protein expression levels of BAX or Bcl-2 at D0 and D21 (P > 0.05) or in the overall survival of subjects with different levels of apoptotic markers. In conclusion, there was no change in BAX or Bcl-2 gene and protein expression in response to ECT at the time points evaluated, but ECT was able to reduce tumor volume and cellular proliferation in cSCC.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-52461-6