Time-course morphological and functional disorders of the kidney induced by long-term high-fat diet intake in female rats

Evidence is emerging that highlights the far-reaching consequences of a high-fat diet (HFD) on kidney morphology and function disorders. The present study was performed on 3-, 5-, 7- and 9-week-old HFD female rats compared with the appropriate gender and age-matched animals. We evaluated the kidney...

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Published in:	Nephrology, dialysis, transplantation Vol. 28; no. 10; pp. 2464 - 2476
Main Authors:	Pinhal, Carolina Staut, Lopes, Agnes, Torres, Daniele Bráz, Felisbino, Sergio Luis, Rocha Gontijo, José Antônio, Boer, Patrícia Aline
Format:	Journal Article
Language:	English
Published:	England 01-10-2013
Subjects:	Animals Biomarkers - analysis Blood Pressure Blotting, Western Diet, High-Fat - adverse effects Epithelial-Mesenchymal Transition Female Fibrosis - etiology Fibrosis - metabolism Fibrosis - pathology Glomerular Filtration Rate Immunoenzyme Techniques Kidney Diseases - etiology Kidney Diseases - metabolism Kidney Diseases - pathology Kidney Function Tests Proteinuria - etiology Proteinuria - metabolism Proteinuria - pathology Rats Rats, Wistar Receptors, Angiotensin - metabolism Time Factors renal function TGFβ-1 kidney fibrosis high-fat diet epithelial-to-mesenchymal transition
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Summary:	Evidence is emerging that highlights the far-reaching consequences of a high-fat diet (HFD) on kidney morphology and function disorders. The present study was performed on 3-, 5-, 7- and 9-week-old HFD female rats compared with the appropriate gender and age-matched animals. We evaluated the kidney expression of angiotensin type II receptor and fibrotic and epithelial-to-mesenchymal transition (EMT) markers, by immunoblotting and immunohistochemical and histological techniques, in parallel with kidney function. In the current study, the time-course HFD-treated group showed, by immunoblotting and immunohistochemical analysis, an early time-course increase in the expression of transforming growth factor β-1 (TGFβ-1) in the entire kidney of HFD-treated rats, compared with that observed in the control group. Simultaneously, the study shows a transient increase in the expression of ZEB2 in the HFD whole kidney accompanied by a fall in the E-cadherin expression and increased collagen and fibronectin deposition. A pronounced decrease in fractional urinary sodium excretion was also demonstrated in the long-term HFD-treated rats. The decreased FENa(+) was accompanied by a fall in FEPNa(+) and FEPPNa(+), which occurred in association with significantly decreased CCr and, certainly on the sodium-filtered load. The reduction in the glomerular filtration rate (GFR) occurred in parallel to proteinuria and glomerular desmin overexpression. The results of the current study suggest that podocyte injury in parallel with observed proteinuria and evidence of EMT transformation are associated with long-term loss of kidney function and renal sodium and water retention.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0931-0509 1460-2385
DOI:	10.1093/ndt/gft304