Search Results - "Pimenta, Frederico M."
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Rational Design of an Efficient, Genetically Encodable, Protein-Encased Singlet Oxygen Photosensitizer
Published in Journal of the American Chemical Society (04-02-2015)“…Singlet oxygen, O2(a1Δg), plays a key role in many processes of cell signaling. Limitations in mechanistic studies of such processes are generally associated…”
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Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo
Published in Proceedings of the National Academy of Sciences - PNAS (19-01-2016)“…This paper presents Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST), a small monomeric protein tag, half as large as the green fluorescent…”
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Oxygen-Dependent Photochemistry and Photophysics of "MiniSOG," a Protein-Encased Flavin
Published in Photochemistry and photobiology (01-09-2013)“…Selected photochemical and photophysical parameters of flavin mononucleotide (FMN) have been examined under conditions in which FMN is (1) solvated in a…”
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Effect of chromophore encapsulation on linear and nonlinear optical properties: the case of "miniSOG", a protein-encased flavin
Published in Physical chemistry chemical physics : PCCP (01-01-2014)“…Linear and nonlinear spectroscopic parameters of flavin mononucleotide, FMN, have been examined both experimentally and computationally under conditions in…”
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Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy
Published in Biochemistry (Easton) (02-10-2018)“…Inducible chemical-genetic fluorescent markers are promising tools for live cell imaging requiring high spatiotemporal resolution and low background…”
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Rho MultiBinder, a fluorescent biosensor that reports the activity of multiple GTPases
Published in Biophysical journal (19-09-2023)“…Imaging two or more fluorescent biosensors in the same living cell can reveal the spatiotemporal coordination of protein activities. However, using multiple…”
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Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors
Published in ACS chemical biology (21-09-2018)“…Fluorescent reporters are essential components for the design of optical biosensors that are able to image intracellular analytes in living cells. Herein, we…”
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VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy
Published in Optics express (08-07-2019)“…We developed VIEW-MOD (Versatile Illumination Engine with a Modular Optical Design): a compact, multi-modality microscope, which accommodates multiple…”
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Chromophore Renewal and Fluorogen-Binding Tags: A Match Made to Last
Published in Scientific reports (26-09-2017)“…Fluorogen-binding tags, which activate the fluorescence of a specific chromophore (so-called fluorogen) upon reversible binding, have recently been proposed as…”
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Protein-encapsulated bilirubin: paving the way to a useful probe for singlet oxygen
Published in Photochemical & photobiological sciences (01-04-2015)“…When dissolved in a bulk solvent, bilirubin efficiently removes singlet molecular oxygen, O2(a(1)Δg), through a combination of chemical reactions and by…”
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Singlet-Oxygen-Mediated Cell Death Using Spatially-Localized Two-Photon Excitation of an Extracellular Sensitizer
Published in The journal of physical chemistry. B (30-08-2012)“…Controlling and quantifying the photosensitized production of singlet oxygen are key aspects in mechanistic studies of oxygen-dependent photoinitiated cell…”
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Intracellular singlet oxygen photosensitizers: on the road to solving the problems of sensitizer degradation, bleaching and relocalization
Published in Integrative biology (Cambridge) (01-02-2016)“…Selected singlet oxygen photosensitizers have been examined from the perspective of obtaining a molecule that is sufficiently stable under conditions currently…”
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A new biosensor design reveals conformational changes of single molecules in living cells
Published in Biophysical journal (11-02-2022)Get full text
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Intracellular singlet oxygen photosensitizers: on the road to solving the problems of sensitizer degradation, bleaching and relocalizationElectronic supplementary information (ESI) available: Additional absorption and emission spectra of the sensitizers and emission-based images of cells, all recorded as a function of elapsed irradiation; further details of the approach used to obtain photobleaching quantum yields. See DOI: 10.1039/c5ib00295h
Published 15-02-2016“…Selected singlet oxygen photosensitizers have been examined from the perspective of obtaining a molecule that is sufficiently stable under conditions currently…”
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A Rare Cause of Recurrent Pain in Athletes: Synovial Hemangioma
Published in Video journal of sports medicine (01-11-2023)“…Background: Synovial hemangioma is a rare neoplastic lesion, which can result in recurrent hemarthrosis and pain. It can affect any joint, tendon, or bursa,…”
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