Search Results - "Pigino, Gaia"

Microtubule doublets are double-track railways for intraflagellar transport trains by Stepanek, Ludek, Pigino, Gaia

“…The cilium is a large macromolecular machine that is vital for motility, signaling, and sensing in most eukaryotic cells. Its conserved core structure, the…”
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Structural Biology of Cilia and Intraflagellar Transport by Klena, Nikolai, Pigino, Gaia

“…Cilia are ubiquitous microtubule-based eukaryotic organelles that project from the cell to generate motility or function in cellular signaling. Motile cilia or…”
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The molecular structure of mammalian primary cilia revealed by cryo-electron tomography by Kiesel, Petra, Alvarez Viar, Gonzalo, Tsoy, Nikolai, Maraspini, Riccardo, Gorilak, Peter, Varga, Vladimir, Honigmann, Alf, Pigino, Gaia

“…Primary cilia are microtubule-based organelles that are important for signaling and sensing in eukaryotic cells. Unlike the thoroughly studied motile cilia,…”
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The structural basis of intraflagellar transport at a glance by Jordan, Mareike A., Pigino, Gaia

“…ABSTRACT The intraflagellar transport (IFT) system is a remarkable molecular machine used by cells to assemble and maintain the cilium, a long organelle…”
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The cryo-EM structure of intraflagellar transport trains reveals how dynein is inactivated to ensure unidirectional anterograde movement in cilia by Jordan, Mareike A., Diener, Dennis R., Stepanek, Ludek, Pigino, Gaia

“…Movement of cargos along microtubules plays key roles in diverse cellular processes, from signalling to mitosis. In cilia, rapid movement of ciliary components…”
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The molecular structure of IFT-A and IFT-B in anterograde intraflagellar transport trains by Lacey, Samuel E., Foster, Helen E., Pigino, Gaia

“…Anterograde intraflagellar transport (IFT) trains are essential for cilia assembly and maintenance. These trains are formed of 22 IFT-A and IFT-B proteins that…”
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Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility by Gadadhar, Sudarshan, Alvarez Viar, Gonzalo, Hansen, Jan Niklas, Gong, An, Kostarev, Aleksandr, Ialy-Radio, Côme, Leboucher, Sophie, Whitfield, Marjorie, Ziyyat, Ahmed, Touré, Aminata, Alvarez, Luis, Pigino, Gaia, Janke, Carsten

“…Posttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked…”
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Reorganization of budding yeast cytoplasm upon energy depletion by Marini, Guendalina, Nüske, Elisabeth, Leng, Weihua, Alberti, Simon, Pigino, Gaia

“…Yeast cells, when exposed to stress, can enter a protective state in which cell division, growth, and metabolism are down-regulated. They remain viable in this…”
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Integrative modeling reveals the molecular architecture of the intraflagellar transport A (IFT-A) complex by McCafferty, Caitlyn L, Papoulas, Ophelia, Jordan, Mareike A, Hoogerbrugge, Gabriel, Nichols, Candice, Pigino, Gaia, Taylor, David W, Wallingford, John B, Marcotte, Edward M

“…Intraflagellar transport (IFT) is a conserved process of cargo transport in cilia that is essential for development and homeostasis in organisms ranging from…”
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In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains by van den Hoek, Hugo, Klena, Nikolai, Jordan, Mareike A., Alvarez Viar, Gonzalo, Righetto, Ricardo D., Schaffer, Miroslava, Erdmann, Philipp S., Wan, William, Geimer, Stefan, Plitzko, Jürgen M., Baumeister, Wolfgang, Pigino, Gaia, Hamel, Virginie, Guichard, Paul, Engel, Benjamin D.

“…The cilium is an antenna-like organelle that performs numerous cellular functions, including motility, sensing, and signaling. The base of the cilium contains…”
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In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip by Wingfield, Jenna L., Mekonnen, Betlehem, Mengoni, Ilaria, Liu, Peiwei, Jordan, Mareike, Diener, Dennis, Pigino, Gaia, Lechtreck, Karl

“…ABSTRACT Flagellar assembly depends on intraflagellar transport (IFT), a bidirectional motility of protein carriers, the IFT trains. The trains are periodic…”
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Cryoelectron tomography of radial spokes in cilia and flagella by Pigino, Gaia, Bui, Khanh Huy, Maheshwari, Aditi, Lupetti, Pietro, Diener, Dennis, Ishikawa, Takashi

“…Radial spokes (RSs) are ubiquitous components in the 9 + 2 axoneme thought to be mechanochemical transducers involved in local control of dynein-driven…”
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Cryo-CARE: Content-Aware Image Restoration for Cryo-Transmission Electron Microscopy Data by Buchholz, Tim-Oliver, Jordan, Mareike, Pigino, Gaia, Jug, Florian

“…Multiple approaches to use deep learning for image restoration have recently been proposed. Training such approaches requires well registered pairs of high and…”
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A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia by Quidwai, Tooba, Wang, Jiaolong, Hall, Emma A, Petriman, Narcis A, Leng, Weihua, Kiesel, Petra, Wells, Jonathan N, Murphy, Laura C, Keighren, Margaret A, Marsh, Joseph A, Lorentzen, Esben, Pigino, Gaia, Mill, Pleasantine

“…Intraflagellar transport (IFT) is a highly conserved mechanism for motor-driven transport of cargo within cilia, but how this cargo is selectively transported…”
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Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that connects radial spoke 3 to dynein g and the nexin link by Bazan, Rafał, Schröfel, Adam, Joachimiak, Ewa, Poprzeczko, Martyna, Pigino, Gaia, Wloga, Dorota

“…Ciliary beating requires the coordinated activity of numerous axonemal complexes. The protein composition and role of radial spokes (RS), nexin links (N-DRC)…”
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Fabrication of High Aspect Ratio Gold Nanowires within the Microtubule Lumen by Joshi, Foram M., Viar, Gonzalo Alvarez, Pigino, Gaia, Drechsler, Hauke, Diez, Stefan

“…Gold nanowires have great potential use as interconnects in electronic, photonic, and optoelectronic devices. To date, there are various fabrication strategies…”
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DNAH11 Localization in the Proximal Region of Respiratory Cilia Defines Distinct Outer Dynein Arm Complexes by Dougherty, Gerard W, Loges, Niki T, Klinkenbusch, Judith A, Olbrich, Heike, Pennekamp, Petra, Menchen, Tabea, Raidt, Johanna, Wallmeier, Julia, Werner, Claudius, Westermann, Cordula, Ruckert, Christian, Mirra, Virginia, Hjeij, Rim, Memari, Yasin, Durbin, Richard, Kolb-Kokocinski, Anja, Praveen, Kavita, Kashef, Mohammad A, Kashef, Sara, Eghtedari, Fardin, Häffner, Karsten, Valmari, Pekka, Baktai, György, Aviram, Micha, Bentur, Lea, Amirav, Israel, Davis, Erica E, Katsanis, Nicholas, Brueckner, Martina, Shaposhnykov, Artem, Pigino, Gaia, Dworniczak, Bernd, Omran, Heymut

“…Primary ciliary dyskinesia (PCD) is a recessively inherited disease that leads to chronic respiratory disorders owing to impaired mucociliary clearance…”
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Structure, interaction and nervous connectivity of beta cell primary cilia by Müller, Andreas, Klena, Nikolai, Pang, Song, Garcia, Leticia Elizabeth Galicia, Topcheva, Oleksandra, Aurrecoechea Duran, Solange, Sulaymankhil, Davud, Seliskar, Monika, Mziaut, Hassan, Schöniger, Eyke, Friedland, Daniela, Kipke, Nicole, Kretschmar, Susanne, Münster, Carla, Weitz, Jürgen, Distler, Marius, Kurth, Thomas, Schmidt, Deborah, Hess, Harald F., Xu, C. Shan, Pigino, Gaia, Solimena, Michele

“…Primary cilia are sensory organelles present in many cell types, partaking in various signaling processes. Primary cilia of pancreatic beta cells play pivotal…”
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Comparative structural analysis of eukaryotic flagella and cilia from Chlamydomonas, Tetrahymena, and sea urchins by Pigino, Gaia, Maheshwari, Aditi, Bui, Khanh Huy, Shingyoji, Chikako, Kamimura, Shinji, Ishikawa, Takashi

“…Although eukaryotic flagella and cilia all share the basic 9+2 microtubule-organization of their internal axonemes, and are capable of generating…”
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Three-dimensional mass density mapping of cellular ultrastructure by ptychographic X-ray nanotomography by Diaz, Ana, Malkova, Barbora, Holler, Mirko, Guizar-Sicairos, Manuel, Lima, Enju, Panneels, Valerie, Pigino, Gaia, Bittermann, Anne Greet, Wettstein, Larissa, Tomizaki, Takashi, Bunk, Oliver, Schertler, Gebhard, Ishikawa, Takashi, Wepf, Roger, Menzel, Andreas

“…We demonstrate absolute quantitative mass density mapping in three dimensions of frozen-hydrated biological matter with an isotropic resolution of 180nm. As…”
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