Detection of virulence-associated and regulatory protein genes in association with phage typing of human Vibrio cholerae from several geographical regions of the world

1 Division of Bacteriology, National Institute of Cholera and Enteric Diseases, Kolkata, India 2 Austrian Agency for Health and Food Safety (AGES), Vienna, Austria 3 Instituto Oswaldo Cruz, Rio de Janeiro, Brazil Correspondence B. L. Sarkar bl_sarkar{at}hotmail.com Received December 3, 2008 Accepted...

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Published in:	Journal of medical microbiology Vol. 58; no. 9; pp. 1160 - 1167
Main Authors:	Bhowmick, Tushar Suvra, Das, Mayukh, Ruppitsch, Werner, Stoeger, Anna, Pietzka, Ariane Tatjana, Allerberger, Franz, Rodrigues, D. P, Sarkar, B. L
Format:	Journal Article
Language:	English
Published:	Reading Soc General Microbiol 01-09-2009 Society for General Microbiology
Subjects:	Bacterial Proteins - genetics Bacteriology Bacteriophage Typing Biological and medical sciences Cholera - epidemiology Cholera - microbiology Fundamental and applied biological sciences. Psychology Gene Expression Profiling Gene Expression Regulation, Bacterial - physiology Global Health Humans Infectious diseases Medical sciences Microbiology Miscellaneous Vibrio cholerae - classification Vibrio cholerae - genetics Virology Virulence World Virus Human Typing Gene Microbiology Vibrio cholerae Virulence Bacteria Vibrionaceae Detection Protein Phage
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Summary:	1 Division of Bacteriology, National Institute of Cholera and Enteric Diseases, Kolkata, India 2 Austrian Agency for Health and Food Safety (AGES), Vienna, Austria 3 Instituto Oswaldo Cruz, Rio de Janeiro, Brazil Correspondence B. L. Sarkar bl_sarkar{at}hotmail.com Received December 3, 2008 Accepted May 26, 2009 Vibrio cholerae O1, O139 and occasionally non-O1/non-O139 serogroups are most often responsible for epidemic and pandemic cholera. This study used genotypic patterns of PCR-based detection of virulence-associated and regulatory protein genes, along with phage typing, to characterize 86 V. cholerae strains. Thirty-eight of 53 O1 biotype El Tor strains harboured both tcpA classical and tcpA El Tor genes, and three El Tor strains lacked the V. cholerae O1-specific gene ( Vc-O1 ); three O139 strains contained both Vc-O1 and Vc-O139 genes and seven out of ten non-O1/non-O139 strains possessed the Vc-O1 gene. The latter strains all harboured the virulence-associated genes ctxA , zot , ace , RS1 , hlyA , ompU , rtxA and sxt . Two phage types, T27 and T25, were predominant in strains from different geographical regions of India, whereas more variation in phage susceptibility was observed for tetracycline-resistant strains from Kolkata. These results suggest that the pattern and distribution of virulence genes and phage types of V. cholerae are equally useful and discriminatory in tracing the origin of newly emerging strains.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-2615 1473-5644
DOI:	10.1099/jmm.0.008466-0