ONT read assembly of the black rhino genome

ONT read assembly of the black rhino genome

The black rhinoceros (Diceros bicornis) is an endangered mammal for which a captive breeding program is part of the conservation effort. Black rhinos in zoo's often suffer from chronic infections and heamochromatosis. Furthermore, breeding is hampered by low male fertility. To aid a research pr...

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Bibliographic Details
Published in:BMC genetics Vol. 25; no. 1; p. 27
Main Authors: Kraaijeveld, Ken, Bossers, Koen, Petrusevski, Nikola, Pieterman, Stef, Bruins-van Sonsbeek, Linda G R, Wittink, Floyd
Format: Journal Article
Language:English
Published: England BioMed Central Ltd 05-03-2024
BioMed Central
BMC
Subjects:
Analysis
Animals
Annotations
Biodiversity
Breeding
Captive breeding
Captive wild animals
Chromosomes
Conservation
Data Note
Diceros bicornis
Endangered species
Fertility
Genetic aspects
Genetic Research
Genetics
Genomes
Genomics
Growth
Health aspects
Male
Nose
Perissodactyla - genetics
Persistent Infection
Research Design
Rhinoceros
Whole genome sequencing
Zoo animals
Zoos
Netherlands
Genome assembly
Diceros bicornis
Long reads Whole genome sequencing
Black rhinoceros
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Summary:The black rhinoceros (Diceros bicornis) is an endangered mammal for which a captive breeding program is part of the conservation effort. Black rhinos in zoo's often suffer from chronic infections and heamochromatosis. Furthermore, breeding is hampered by low male fertility. To aid a research project studying these topics, we sequenced and assembled the genome of a captive male black rhino using ONT sequencing data only. This work produced over 100 Gb whole genome sequencing reads from whole blood. These were assembled into a 2.47 Gb draft genome consisting of 834 contigs with an N50 of 29.53 Mb. The genome annotation was lifted over from an available genome annotation for black rhino, which resulted in the retrieval of over 99% of gene features. This new genome assembly will be a valuable resource in for conservation genetic research in this species.
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ISSN:2730-6844
2730-6844
1471-2156
DOI:10.1186/s12863-024-01214-0