A Novel, Highly Related Jumbo Family of Bacteriophages That Were Isolated Against Erwinia

A Novel, Highly Related Jumbo Family of Bacteriophages That Were Isolated Against Erwinia

is a plant pathogen from the family and a causative agent of the devastating agricultural disease fire blight. Here we characterize eight lytic bacteriophages of that we isolated from the Wasatch front (Utah, United States) that are highly similar to vB_EamM_Ea35-70 which was isolated in Ontario, Ca...

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Bibliographic Details
Published in:Frontiers in microbiology Vol. 10; p. 1533
Main Authors: Sharma, Ruchira, Pielstick, Brittany A, Bell, Kimberly A, Nieman, Tanner B, Stubbs, Olivia A, Yeates, Edward L, Baltrus, David A, Grose, Julianne H
Format: Journal Article
Language:English
Published: Switzerland Frontiers Media S.A 23-07-2019
Subjects:
Agrican357virus
burst size
Erwinia
jumbo bacteriophage
Microbiology
novel
Pantoea
burst size
genome
Erwinia
Pantoea
jumbo bacteriophage
Agrican357virus
novel
proteome
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Summary:is a plant pathogen from the family and a causative agent of the devastating agricultural disease fire blight. Here we characterize eight lytic bacteriophages of that we isolated from the Wasatch front (Utah, United States) that are highly similar to vB_EamM_Ea35-70 which was isolated in Ontario, Canada. With the genome size ranging from 271 to 275 kb, this is a novel jumbo family of bacteriophages. These jumbo bacteriophages were further characterized through genomic and proteomic comparison, mass spectrometry, host range and burst size. Their proteomes are highly unstudied, with over 200 putative proteins with no known homologs. The production of 27 of these putative proteins was confirmed by mass spectrometry analysis. These bacteriophages appear to be most similar to bacteriophages that infect and rather than bacteria by protein similarity, however, we were only able to detect infection of and the closely related strains of .
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Edited by: Manuel Martinez Garcia, University of Alicante, Spain
This article was submitted to Virology, a section of the journal Frontiers in Microbiology
Reviewed by: Jeroen Wagemans, KU Leuven, Belgium; Ana-Belen Martin-Cuadrado, University of Alicante, Spain; Lars Fieseler, Zurich University of Applied Sciences, Switzerland
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2019.01533