Search Results - "Pichot, Florian"

RNA ribose methylation (2′-O-methylation): Occurrence, biosynthesis and biological functions by Ayadi, Lilia, Galvanin, Adeline, Pichot, Florian, Marchand, Virginie, Motorin, Yuri

“…Methylation of riboses at 2′-OH group is one of the most common RNA modifications found in number of cellular RNAs from almost any species which belong to all…”
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HydraPsiSeq: a method for systematic and quantitative mapping of pseudouridines in RNA by Marchand, Virginie, Pichot, Florian, Neybecker, Paul, Ayadi, Lilia, Bourguignon-Igel, Valérie, Wacheul, Ludivine, Lafontaine, Denis L J, Pinzano, Astrid, Helm, Mark, Motorin, Yuri

“…Abstract Developing methods for accurate detection of RNA modifications remains a major challenge in epitranscriptomics. Next-generation sequencing-based…”
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Holistic Optimization of Bioinformatic Analysis Pipeline for Detection and Quantification of 2'-O-Methylations in RNA by RiboMethSeq by Pichot, Florian, Marchand, Virginie, Ayadi, Lilia, Bourguignon-Igel, Valérie, Helm, Mark, Motorin, Yuri

“…A major trend in the epitranscriptomics field over the last 5 years has been the high-throughput analysis of RNA modifications by a combination of specific…”
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Next-Generation Sequencing-Based RiboMethSeq  Protocol for Analysis of tRNA 2'-O-Methylation by Marchand, Virginie, Pichot, Florian, Thüring, Kathrin, Ayadi, Lilia, Freund, Isabel, Dalpke, Alexander, Helm, Mark, Motorin, Yuri

“…Analysis of RNA modifications by traditional physico-chemical approaches is labor  intensive,  requires  substantial  amounts  of  input  material  and  only …”
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Quantification of substoichiometric modification reveals global tsRNA hypomodification, preferences for angiogenin-mediated tRNA cleavage, and idiosyncratic epitranscriptomes of human neuronal cell-lines by Pichot, Florian, Hogg, Marion C., Marchand, Virginie, Bourguignon, Valérie, Jirström, Elisabeth, Farrell, Cliona, Gibriel, Hesham A., Prehn, Jochen H.M., Motorin, Yuri, Helm, Mark

“…Modification of tRNA is an integral part of the epitranscriptome with a particularly pronounced potential to generate diversity in RNA expression. Eukaryotic…”
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Machine learning algorithm for precise prediction of 2′-O-methylation (Nm) sites from experimental RiboMethSeq datasets by Pichot, Florian, Marchand, Virginie, Helm, Mark, Motorin, Yuri

“…•Improved mapping of 2′-O-methylated (Nm) and pseudouridine (ψ) residues by RiboMethSeq.•Random Forest (RF) model including scores for neighboring positions…”
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Non-Redundant tRNA Reference Sequences for Deep Sequencing Analysis of tRNA Abundance and Epitranscriptomic RNA Modifications by Pichot, Florian, Marchand, Virginie, Helm, Mark, Motorin, Yuri

“…Analysis of RNA by deep-sequencing approaches has found widespread application in modern biology. In addition to measurements of RNA abundance under various…”
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Data Analysis Pipeline for Detection and Quantification of Pseudouridine (ψ) in RNA by HydraPsiSeq by Pichot, Florian, Marchand, Virginie, Helm, Mark, Motorin, Yuri

“…Pseudouridine, a modified RNA residue formed by the isomerization of its parental U nucleotide, is prevalent in a majority of cellular RNAs; its presence was…”
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N1-methylation of adenosine (m1A) in ND5 mRNA leads to complex I dysfunction in Alzheimer’s disease by Jörg, Marko, Plehn, Johanna E., Kristen, Marco, Lander, Marc, Walz, Lukas, Lietz, Christine, Wijns, Julie, Pichot, Florian, Rojas-Charry, Liliana, Wirtz Martin, Katja M., Ruffini, Nicolas, Kreim, Nastasja, Gerber, Susanne, Motorin, Yuri, Endres, Kristina, Rossmanith, Walter, Methner, Axel, Helm, Mark, Friedland, Kristina

“…One mechanism of particular interest to regulate mRNA fate post-transcriptionally is mRNA modification. Especially the extent of m 1 A mRNA methylation is…”
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2'- O -methylation within prokaryotic and eukaryotic tRNA inhibits innate immune activation by endosomal Toll-like receptors but does not affect recognition of whole organisms by Freund, Isabel, Buhl, Daniel K, Boutin, Sébastien, Kotter, Annika, Pichot, Florian, Marchand, Virginie, Vierbuchen, Tim, Heine, Holger, Motorin, Yuri, Helm, Mark, Dalpke, Alexander H, Eigenbrod, Tatjana

“…Bacterial RNA has emerged as an important activator of innate immune responses by stimulating Toll-like receptors TLR7 and TLR8 in humans. Guanosine 2'-…”
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NOseq: amplicon sequencing evaluation method for RNA m6A sites after chemical deamination by Werner, Stephan, Galliot, Aurellia, Pichot, Florian, Kemmer, Thomas, Marchand, Virginie, Sednev, Maksim V, Lence, Tina, Roignant, Jean-Yves, König, Julian, Höbartner, Claudia, Motorin, Yuri, Hildebrandt, Andreas, Helm, Mark

“…Abstract Methods for the detection of m6A by RNA-Seq technologies are increasingly sought after. We here present NOseq, a method to detect m6A residues in…”
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