Search Results - "Phillips, Nelson F.B"

Development of glucose-responsive 'smart' insulin systems by Rege, Nischay K, Phillips, Nelson F B, Weiss, Michael A

“…The complexity of modern insulin-based therapy for type I and type II diabetes mellitus and the risks associated with excursions in blood-glucose concentration…”
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Connecting Rodent and Human Pharmacokinetic Models for the Design and Translation of Glucose-Responsive Insulin by Yang, Jing Fan, Gong, Xun, Bakh, Naveed A, Carr, Kelley, Phillips, Nelson F B, Ismail-Beigi, Faramarz, Weiss, Michael A, Strano, Michael S

“…Despite considerable progress, development of glucose-responsive insulins (GRIs) still largely depends on empirical knowledge and tedious…”
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“Register-shift” insulin analogs uncover constraints of proteotoxicity in protein evolution by Rege, Nischay K., Liu, Ming, Dhayalan, Balamurugan, Chen, Yen-Shan, Smith, Nicholas A., Rahimi, Leili, Sun, Jinhong, Guo, Huan, Yang, Yanwu, Haataja, Leena, Phillips, Nelson F.B., Whittaker, Jonathan, Smith, Brian J., Arvan, Peter, Ismail-Beigi, Faramarz, Weiss, Michael A.

“…Globular protein sequences encode not only functional structures (the native state) but also protein foldability, i.e. a conformational search that is both…”
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Structure-based stabilization of insulin as a therapeutic protein assembly via enhanced aromatic–aromatic interactions by Rege, Nischay K., Wickramasinghe, Nalinda P., Tustan, Alisar N., Phillips, Nelson F.B., Yee, Vivien C., Ismail-Beigi, Faramarz, Weiss, Michael A.

“…Key contributions to protein structure and stability are provided by weakly polar interactions, which arise from asymmetric electronic distributions within…”
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Isolation of a pyrophosphate-dependent phosphofructokinase from Hexamita inflata by Phillips, Nelson F.B, Li, Zujin, Lindmark, Donald G

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A Protein Caught in a Kinetic Trap:  Structures and Stabilities of Insulin Disulfide Isomers by Hua, Qing-Xin, Jia, Wenhua, Frank, Bruce H, Phillips, Nelson F. B, Weiss, Michael A

“…Proinsulin contains six cysteines whose specific pairing (A6−A11, A7−B7, and A20−B19) is a defining feature of the insulin fold. Pairing information is…”
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Mechanism of insulin chain combination. Asymmetric roles of A-chain alpha-helices in disulfide pairing by Hua, Qing-Xin, Chu, Ying-Chi, Jia, Wenhua, Phillips, Nelson F B, Wang, Run-Ying, Katsoyannis, Panayotis G, Weiss, Michael A

“…The A and B chains of insulin combine to form native disulfide bridges without detectable isomers. The fidelity of chain combination thus recapitulates the…”
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Mechanism of Insulin Chain Combination by Hua, Qing-xin, Chu, Ying-Chi, Jia, Wenhua, Phillips, Nelson F.B., Wang, Run-ying, Katsoyannis, Panayotis G., Weiss, Michael A.

“…The A and B chains of insulin combine to form native disulfide bridges without detectable isomers. The fidelity of chain combination thus recapitulates the…”
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Cloning, Expression, and Characterization of Polyphosphate Glucokinase from Mycobacterium tuberculosis by Hsieh, P C, Shenoy, B C, Samols, D, Phillips, N F

“…Polyphosphate glucokinase from Mycobacterium tuberculosis catalyzes the phosphorylation of glucose using polyphosphate or ATP as the phosphoryl donor. The M…”
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Kinetic Mechanisms of Polyphosphate Glucokinase from Mycobacterium tuberculosis by Hsieh, Pei-Chung, Kowalczyk, Tomasz H, Phillips, Nelson F. B

“…Polyphosphate glucokinase from Mycobacterium tuberculosis catalyzes the phosphorylation of glucose using inorganic polyphosphates [poly(P)] or ATP. The…”
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Kinetic mechanism of pyrophosphate-dependent phosphofructokinase from Giardia lamblia by Phillips, Nelson F.B., Li, Zujin

“…The steady-state kinetics of the reaction catalyzed by inorganic-pyrophosphate-dependent d-fructose-6-phosphate 1-phosphotransferase from Giardia lamblia have…”
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The polyphosphate- and ATP-dependent glucokinase from Propionibacterium shermanii: both activities are catalyzed by the same protein by Phillips, N F, Horn, P J, Wood, H G

“…The glucokinase (EC 2.7.1.63) from Propionibacterium shermanii phosphorylates glucose using inorganic polyphosphate (poly(P)) or ATP as the phosphate donor. In…”
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Pyrophosphate-Dependent Phosphofructokinase from Giardia lamblia: Purification and Characterization by Li, Z.J., Phillips, N.F.B.

“…The pyrophosphate-dependent phosphofructokinase (EC 2.7.1.90) from Giardia lamblia has been purified to homogeneity using two methods. The purified enzyme is…”
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Purification of polyphosphate and ATP glucose phosphotransferase from Mycobacterium tuberculosis H37Ra: evidence that poly(P) and ATP glucokinase activities are catalyzed by the same enzyme by Hsieh, P C, Shenoy, B C, Jentoft, J E, Phillips, N F

“…Polyphosphate [poly(P)n]:D-(+)-glucose-6-phosphotransferase (EC 2.7.1.63) from Mycobacterium tuberculosis H37Ra was purified to homogeneity using an improved…”
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Initial Rate and Equilibrium Isotope Exchange Studies on the ATP-Dependent Activity of Polyphosphate Glucokinase from Propionibacterium shermanii by Kowalczyk, Tomasz H, Horn, Peter J, Pan, Wei-Hua, Phillips, Nelson F. B

“…Polyphosphate glucokinase [EC 2.7.1.63] catalyzes the phosphorylation of glucose using either inorganic polyphosphate [poly(P)] or ATP as the phosphoryl donor…”
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Isolation of a carboxyphosphate intermediate and the locus of acetyl-CoA action in the pyruvate carboxylase reaction by Phillips, Nelson F. B, Snoswell, Mark A, Chapman-Smith, Anne, Keech, D. Bruce, Wallace, John C

“…When chicken liver pyruvate carboxylase was incubated with either H14CO3- or gamma-[32P]ATP, a labeled carboxyphospho-enzyme intermediate could be isolated…”
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Determination of endopolyphosphatase using polyphosphate glucokinase by Kowalczyk, T H, Phillips, N F

“…A method has been developed for determining endopolyphosphatase (polyphosphate depolymerase, EC 3.6.1.10) activity. The enzyme catalyzes the hydrolysis of…”
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Primary structure of the 5 S subunit of transcarboxylase as deduced from the genomic DNA sequence by Thornton, Charles G., Kumar, Ganesh K., Shenoy, Bhami C., Haase, F.Carl, Phillips, Nelson F.B., Park, Vicki M., Magner, William J., Hejlik, Daniel P., Wood, Harland G., Samols, David

“…Transcarboxylase from Propionibacterium shermanii is a complex biotin-containing enzyme composed of 30 polypeptides of three different types. It is composed of…”
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The ATP/AMP binding site of pyruvate, phosphate dikinase: selective modification with fluorescein isothiocyanate by Phillips, Nelson F. B

“…Pyruvate,phosphate dikinase from Propionibacterium shermanii is strongly inhibited by fluorescein 5'-isothiocyanate (FITC). The time course of inactivation is…”
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Involvement of tryptophan(s) at the active site of polyphosphate/ATP glucokinase from Mycobacterium tuberculosis by Hsieh, Pei Chung, Shenoy, Bhami C, Haase, F. Carl, Jentoft, Joyce E, Phillips, Nelson F. B

“…The glucokinase (EC 2.7.1.63) from Mycobacterium tuberculosis catalyzes the phosphorylation of glucose using inorganic polyphosphate (poly(P)) or ATP as the…”
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