Search Results - "Phelan, Ryan"

Saving the horseshoe crab: A synthetic alternative to horseshoe crab blood for endotoxin detection by Maloney, Tom, Phelan, Ryan, Simmons, Naira

“…Horseshoe crabs have been integral to the safe production of vaccines and injectable medications for the past 40 years. The bleeding of live horseshoe crabs, a…”
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U.S. conservation translocations: Over a century of intended consequences by Novak, Ben J., Phelan, Ryan, Weber, Michele

“…Conservation translocations (reintroductions, reinforcements, ecological replacements, and assisted colonization) have played a vital and necessary role in…”
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Mechanistic Insights into the Bifunctional Non-Heme Iron Oxygenase Carbapenem Synthase by Active Site Saturation Mutagenesis by Phelan, Ryan M, Townsend, Craig A

“…The carbapenem class of β-lactam antibiotics is known for its remarkable potency, antibacterial spectrum, and resistance to β-lactamase-mediated inactivation…”
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Development of Next Generation Synthetic Biology Tools for Use in Streptomyces venezuelae by Phelan, Ryan M, Sachs, Daniel, Petkiewicz, Shayne J, Barajas, Jesus F, Blake-Hedges, Jacquelyn M, Thompson, Mitchell G, Reider Apel, Amanda, Rasor, Blake J, Katz, Leonard, Keasling, Jay D

“…Streptomyces have a rich history as producers of important natural products and this genus of bacteria has recently garnered attention for its potential…”
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Solid, Noncovalent Formulation of Biocatalysts for Rapid and Accurate Submilligram Dosing to Microtiter Plates by Brown, Jesse T. C, Tu, Noah P, Phelan, Ryan M

“…High-throughput experimentation has become increasingly important in the identification and optimization of enzymatic reactions. Unfortunately, traditional…”
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externally tunable bacterial band-pass filter by Sohka, Takayuki, Heins, Richard A, Phelan, Ryan M, Greisler, Jennifer M, Townsend, Craig A, Ostermeier, Marc

“…The current paradigm for tuning synthetic biological systems is through re-engineering system components. Biological systems designed with the inherent ability…”
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Production of Odd-Carbon Dicarboxylic Acids in Escherichia coli Using an Engineered Biotin–Fatty Acid Biosynthetic Pathway by Haushalter, Robert W, Phelan, Ryan M, Hoh, Kristina M, Su, Cindy, Wang, George, Baidoo, Edward E. K, Keasling, Jay D

“…Dicarboxylic acids are commodity chemicals used in the production of plastics, polyesters, nylons, fragrances, and medications. Bio-based routes to…”
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Response to Strayer (2022) by Novak, Ben J., Phelan, Ryan, Weber, Michele

“…In his critique, Strayer ( 2022) failed to provide a single case of a conservation translocation resulting in harm to an ecosystem to counter our findings. In…”
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Divergent Mechanistic Routes for the Formation of gem-Dimethyl Groups in the Biosynthesis of Complex Polyketides by Poust, Sean, Phelan, Ryan M., Deng, Kai, Katz, Leonard, Petzold, Christopher J., Keasling, Jay D.

“…The gem‐dimethyl groups in polyketide‐derived natural products add steric bulk and, accordingly, lend increased stability to medicinal compounds, however, our…”
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Why intended consequences? by Phelan, Ryan, Kareiva, Peter, Marvier, Michelle, Robbins, Paul, Weber, Michele

“…To counter the focus on potential unintended consequences, we hope that the catchphrase “Intended Consequences” will help to keep the intended benefits to…”
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Non-Heme Iron Oxygenases Generate Natural Structural Diversity in Carbapenem Antibiotics by Bodner, Micah J, Phelan, Ryan M, Freeman, Michael F, Li, Rongfeng, Townsend, Craig A

“…Carbapenems are a clinically important antibiotic family. More than 50 naturally occurring carbapenam/ems are known and are distinguished primarily by their…”
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Definition of the Common and Divergent Steps in Carbapenem β-Lactam Antibiotic Biosynthesis by Bodner, Micah J., Li, Rongfeng, Phelan, Ryan M., Freeman, Michael F., Moshos, Kristos A., Lloyd, Evan P., Townsend, Craig A.

“…Approximately 50 naturally occurring carbapenem β‐lactam antibiotics are known. All but one of these have been isolated from Streptomyces species and are…”
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A Catalytic Asymmetric Route to Carbapenems by Bodner, Micah J, Phelan, Ryan M, Townsend, Craig A

“…Efficient syntheses of N-acetyl thienamycin and epithienamycin A in their readily deprotected form are reported where three contiguous stereocenters are…”
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A High-Throughput Screen for the Engineered Production of β-Lactam Antibiotics by Phelan, Ryan M, DiPardo, Benjamin J, Townsend, Craig A

“…High-throughput screens and selections have had profound impact on our ability to engineer proteins possessing new, desired properties. These methods are…”
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Design and synthesis of a β-lactamase activated 5-fluorouracil prodrug by Phelan, Ryan M., Ostermeier, Marc, Townsend, Craig A.

“…An efficient synthesis of a 5-fluorouracil-cephalosporin prodrug is described for use against colorectal and other cancers in antibody and gene-directed…”
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Engineering Terpene Biosynthesis in Streptomyces for Production of the Advanced Biofuel Precursor Bisabolene by Phelan, Ryan M, Sekurova, Olga N, Keasling, Jay D, Zotchev, Sergey B

“…The past decade has witnessed a large influx of research toward the creation of sustainable, biologically derived fuels. While significant effort has been…”
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Divergent Mechanistic Routes for the Formation of gem-Dimethyl Groups in the Biosynthesis of Complex Polyketides by Poust, Sean, Phelan, Ryan M., Deng, Kai, Katz, Leonard, Petzold, Christopher J., Keasling, Jay D.

“…The gem‐dimethyl groups in polyketide‐derived natural products add steric bulk and, accordingly, lend increased stability to medicinal compounds, however, our…”
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Comparison of Diverse Amine Donors for Use in the High-Throughput Screening of Amine Transaminases by Brown, Jesse T. C., Phelan, Ryan M.

“…Chiral amines are important structural features of many pharmaceutical and agrochemical compounds; accordingly, numerous synthetic methods have been developed…”
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Comprehensive Structural and Biochemical Analysis of the Terminal Myxalamid Reductase Domain for the Engineered Production of Primary Alcohols by Barajas, Jesus F., Phelan, Ryan M., Schaub, Andrew J., Kliewer, Jaclyn T., Kelly, Peter J., Jackson, David R., Luo, Ray, Keasling, Jay D., Tsai, Shiou-Chuan

“…The terminal reductase (R) domain from the non-ribosomal peptide synthetase (NRPS) module MxaA in Stigmatella aurantiaca Sga15 catalyzes a non-processive…”
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Is It Time for Synthetic Biodiversity Conservation? by Piaggio, Antoinette J., Segelbacher, Gernot, Seddon, Philip J., Alphey, Luke, Bennett, Elizabeth L., Carlson, Robert H., Friedman, Robert M., Kanavy, Dona, Phelan, Ryan, Redford, Kent H., Rosales, Marina, Slobodian, Lydia, Wheeler, Keith

“…Evidence indicates that, despite some critical successes, current conservation approaches are not slowing the overall rate of biodiversity loss. The field of…”
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