Search Results - "Pfleger, Kevin D.G."

G Protein-Coupled Receptor Heteromers by Gomes, Ivone, Ayoub, Mohammed Akli, Fujita, Wakako, Jaeger, Werner C, Pfleger, Kevin D.G, Devi, Lakshmi A

“…G protein-coupled receptors (GPCRs) compose one of the largest families of membrane proteins involved in intracellular signaling. They are involved in numerous…”
Get full text

Transactivation of RAGE mediates angiotensin-induced inflammation and atherogenesis by Pickering, Raelene J, Tikellis, Christos, Rosado, Carlos J, Tsorotes, Despina, Dimitropoulos, Alexandra, Smith, Monique, Huet, Olivier, Seeber, Ruth M, Abhayawardana, Rekhati, Johnstone, Elizabeth Km, Golledge, Jonathan, Wang, Yutang, Jandeleit-Dahm, Karin A, Cooper, Mark E, Pfleger, Kevin Dg, Thomas, Merlin C

“…Activation of the type 1 angiotensin II receptor (AT1) triggers proinflammatory signaling through pathways independent of classical Gq signaling that regulate…”
Get full text

G-protein coupled receptor oligomerization in neuroendocrine pathways by Kroeger, Karen M, Pfleger, Kevin D.G, Eidne, Karin A

“…Protein–protein interactions are fundamental processes for many biological systems including those involving the superfamily of G-protein coupled receptors…”
Get full text

Application of BRET to monitor ligand binding to GPCRs by Stoddart, Leigh A, Johnstone, Elizabeth K M, Wheal, Amanda J, Goulding, Joëlle, Robers, Matthew B, Machleidt, Thomas, Wood, Keith V, Hill, Stephen J, Pfleger, Kevin D G

“…Bioluminescence resonance energy transfer, from the substrate of a luciferase fused with a G protein–coupled receptor to a fluorescent dye covalently linked to…”
Get full text

Differential association modes of the thrombin receptor PAR₁ with Gαi1, Gα12, and β-arrestin 1 by Ayoub, Mohammed Akli, Trinquet, Eric, Pfleger, Kevin D.G, Pin, Jean-Philippe

“…Although many G protein-coupled receptors (GPCRs) are known to activate multiple signaling pathways by coupling to different types of G proteins or by…”
Get full text

BRET-based assay to monitor EGFR transactivation by the AT1R reveals Gq/11 protein-independent activation and AT1R-EGFR complexes by O'Brien, Shannon L., Johnstone, Elizabeth K.M., Devost, Dominic, Conroy, Jacinta, Reichelt, Melissa E., Purdue, Brooke W., Ayoub, Mohammed A., Kawai, Tatsuo, Inoue, Asuka, Eguchi, Satoru, Hébert, Terence E., Pfleger, Kevin D.G., Thomas, Walter G.

“…[Display omitted] The type 1 angiotensin II (AngII) receptor (AT1R) transactivates the epidermal growth factor receptor (EGFR), which leads to pathological…”
Get full text

Effect of enhanced Renilla luciferase and fluorescent protein variants on the Förster distance of Bioluminescence resonance energy transfer (BRET) by Dacres, Helen, Michie, Michelle, Wang, Jian, Pfleger, Kevin D.G., Trowell, Stephen C.

“…► First experimental determination of Förster distance (R0) for enhanced BRET systems. ► Effect of brighter BRET components RLuc2, RLuc8 and Venus was…”
Get full text

Disease‐associated missense variants in ZBTB18 disrupt DNA binding and impair the development of neurons within the embryonic cerebral cortex by Hemming, Isabel A., Clément, Olivier, Gladwyn‐Ng, Ivan E., Cullen, Hayley D., Ng, Han Leng, See, Heng B., Ngo, Linh, Ulgiati, Daniela, Pfleger, Kevin D.G., Agostino, Mark, Heng, Julian I‐T.

“…The activities of DNA‐binding transcription factors, such as the multi‐zinc‐finger protein ZBTB18 (also known as RP58, or ZNF238), are essential to coordinate…”
Get full text

Heteromerization of angiotensin receptors changes trafficking and arrestin recruitment profiles by Porrello, Enzo R., Pfleger, Kevin D.G., Seeber, Ruth M., Qian, Hongwei, Oro, Cristina, Abogadie, Fe, Delbridge, Lea M.D., Thomas, Walter G.

“…The cardiovascular hormone angiotensin II (AngII) exerts its actions via two G protein-coupled receptor (GPCR) subtypes, AT 1 and AT 2, which often display…”
Get full text

Temporal Profiling of Orexin Receptor-Arrestin-Ubiquitin Complexes Reveals Differences between Receptor Subtypes by Dalrymple, Matthew B., Jaeger, Werner C., Eidne, Karin A., Pfleger, Kevin D.G.

“…Orexin G protein-coupled receptors (OxRs) and their cognate agonists have been implicated in a number of disorders since their recent discovery, ranging from…”
Get full text

A nanoluciferase biosensor to investigate endogenous chemokine secretion and receptor binding by White, Carl W., Kilpatrick, Laura E., Pfleger, Kevin D.G., Hill, Stephen J.

“…Secreted chemokines are critical mediators of cellular communication that elicit intracellular signaling by binding membrane-bound receptors. Here we…”
Get full text

Biocompatibility of semiconducting AlGaN/GaN material with living cells by Podolska, Anna, Tham, Stephanie, Hart, Robert D., Seeber, Ruth M., Kocan, Martin, Kocan, Martina, Mishra, Umesh K., Pfleger, Kevin D.G., Parish, Gia, Nener, Brett D.

“…With the aim of developing a highly sensitive, mass producible biosensor, we have investigated the growth of human embryonic kidney (HEK) 293 cells on the…”
Get full text

Demonstration of improvements to the bioluminescence resonance energy transfer (BRET) technology for the monitoring of G protein-coupled receptors in live cells by Kocan, Martina, See, Heng B, Seeber, Ruth M, Eidne, Karin A, Pfleger, Kevin D G

“…The bioluminescence resonance energy transfer (BRET) technique has become extremely popular for studying protein-protein interactions in living cells and real…”
Get more information

Application of G protein-coupled receptor-heteromer identification technology to monitor β-arrestin recruitment to G protein-coupled receptor heteromers by See, Heng B, Seeber, Ruth M, Kocan, Martina, Eidne, Karin A, Pfleger, Kevin D G

“…Understanding the role of G protein-coupled receptor (GPCR; also known as a 7 transmembrane receptor) heteromerization in the physiology and pathophysiology of…”
Get more information

The molecular structure and role of CCL2 (MCP‐1) and C‐C chemokine receptor CCR2 in skeletal biology and diseases by Zhu, Sipin, Liu, Mei, Bennett, Samuel, Wang, Ziyi, Pfleger, Kevin D. G., Xu, Jiake

“…Monocyte chemoattractant protein‐1, also called chemokine (C‐C motif) ligand 2 (CCL2) or small inducible cytokine A2, is an inflammatory mediator capable of…”
Get full text

CRISPR-Mediated Protein Tagging with Nanoluciferase to Investigate Native Chemokine Receptor Function and Conformational Changes by White, Carl W, Caspar, Birgit, Vanyai, Hannah K, Pfleger, Kevin D G, Hill, Stephen J

“…G protein-coupled receptors are a major class of membrane receptors that mediate physiological and pathophysiological cellular signaling. Many aspects of…”
Get more information

Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET) by Pfleger, Kevin D G, Eidne, Karin A

“…Bioluminescence resonance energy transfer (BRET) is a straightforward biophysical technique for studying protein-protein interactions. It requires: (1) that…”
Get full text

Fluorescence‐ and bioluminescence‐based approaches to study GPCR ligand binding by Stoddart, Leigh A, White, Carl W, Nguyen, Kim, Hill, Stephen J, Pfleger, Kevin D G

“…Ligand binding is a vital component of any pharmacologist's toolbox and allows the detailed investigation of how a molecule binds to its receptor. These…”
Get full text

NanoBRET ligand binding at a GPCR under endogenous promotion facilitated by CRISPR/Cas9 genome editing by White, Carl W., Johnstone, Elizabeth K.M., See, Heng B., Pfleger, Kevin D.G.

“…Bioluminescence resonance energy transfer (BRET) is a versatile tool used to investigate membrane receptor signalling and function. We have recently developed…”
Get full text

Profiling novel pharmacology of receptor complexes using Receptor-HIT by Johnstone, Elizabeth K M, Pfleger, Kevin D G

“…Many receptors are able to undergo heteromerisation, leading to the formation of receptor complexes that may have pharmacological profiles distinct from those…”
Get more information