Search Results - "Pezzulo, Alejandro"

Quantitative Chest CT Assessment of Small Airways Disease in Post-Acute SARS-CoV-2 Infection by Cho, Josalyn L, Villacreses, Raul, Nagpal, Prashant, Guo, Junfeng, Pezzulo, Alejandro A, Thurman, Andrew L, Hamzeh, Nabeel Y, Blount, Robert J, Fortis, Spyridon, Hoffman, Eric A, Zabner, Joseph, Comellas, Alejandro P

“…Background The long-term effects of SARS-CoV-2 infection on pulmonary structure and function remain incompletely characterized. Purpose To test whether…”
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Eicosanoid signalling blockade protects middle-aged mice from severe COVID-19 by Wong, Lok-Yin Roy, Zheng, Jian, Wilhelmsen, Kevin, Li, Kun, Ortiz, Miguel E., Schnicker, Nicholas J., Thurman, Andrew, Pezzulo, Alejandro A., Szachowicz, Peter J., Li, Pengfei, Pan, Ruangang, Klumpp, Klaus, Aswad, Fred, Rebo, Justin, Narumiya, Shuh, Murakami, Makoto, Zuniga, Sonia, Sola, Isabel, Enjuanes, Luis, Meyerholz, David K., Fortney, Kristen, McCray, Paul B., Perlman, Stanley

“…Coronavirus disease 2019 (COVID-19) is especially severe in aged populations 1 . Vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)…”
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Reduced airway surface pH impairs bacterial killing in the porcine cystic fibrosis lung by Pezzulo, Alejandro A., Tang, Xiao Xiao, Hoegger, Mark J., Abou Alaiwa, Mahmoud H., Ramachandran, Shyam, Moninger, Thomas O., Karp, Phillip H., Wohlford-Lenane, Christine L., Haagsman, Henk P., van Eijk, Martin, Bánfi, Botond, Horswill, Alexander R., Stoltz, David A., McCray, Paul B., Welsh, Michael J., Zabner, Joseph

“…In a porcine cystic fibrosis model, lack of cystic fibrosis transmembrane conductance regulator (CFTR) is shown to result in acidification of airway surface…”
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Measles virus exits human airway epithelia within dislodged metabolically active infectious centers by Hippee, Camilla E, Singh, Brajesh K, Thurman, Andrew L, Cooney, Ashley L, Pezzulo, Alejandro A, Cattaneo, Roberto, Sinn, Patrick L

“…Measles virus (MeV) is the most contagious human virus. Unlike most respiratory viruses, MeV does not directly infect epithelial cells upon entry in a new…”
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Paracellular bicarbonate flux across human cystic fibrosis airway epithelia tempers changes in airway surface liquid pH by Thornell, Ian M., Rehman, Tayyab, Pezzulo, Alejandro A., Welsh, Michael J.

“…Key points Cl− and HCO3− had similar paracellular permeabilities in human airway epithelia. PCl/PNa of airway epithelia was unaltered by pH 7.4 vs. pH 6.0…”
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Heterogeneous expression of the SARS-Coronavirus-2 receptor ACE2 in the human respiratory tract by Ortiz, Miguel E., Thurman, Andrew, Pezzulo, Alejandro A., Leidinger, Mariah R., Klesney-Tait, Julia A., Karp, Philip H., Tan, Ping, Wohlford-Lenane, Christine, McCray, Paul B., Meyerholz, David K.

“…Zoonotically transmitted coronaviruses are responsible for three disease outbreaks since 2002, including the current COVID-19 pandemic, caused by SARS-CoV-2…”
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Differential gene expression analysis for multi-subject single-cell RNA-sequencing studies with aggregateBioVar by Thurman, Andrew L, Ratcliff, Jason A, Chimenti, Michael S, Pezzulo, Alejandro A

“…Abstract Motivation Single-cell RNA-sequencing (scRNA-seq) provides more granular biological information than bulk RNA-sequencing; bulk RNA sequencing remains…”
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The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia by Pezzulo, Alejandro A, Starner, Timothy D, Scheetz, Todd E, Traver, Geri L, Tilley, Ann E, Harvey, Ben-Gary, Crystal, Ronald G, McCray, Jr, Paul B, Zabner, Joseph

“…Organotypic cultures of primary human airway epithelial cells have been used to investigate the morphology, ion and fluid transport, innate immunity,…”
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Inflammatory cytokines TNF-α and IL-17 enhance the efficacy of cystic fibrosis transmembrane conductance regulator modulators by Rehman, Tayyab, Karp, Philip H, Tan, Ping, Goodell, Brian J, Pezzulo, Alejandro A, Thurman, Andrew L, Thornell, Ian M, Durfey, Samantha L, Duffey, Michael E, Stoltz, David A, McKone, Edward F, Singh, Pradeep K, Welsh, Michael J

“…Without cystic fibrosis transmembrane conductance regulator-mediated (CFTR-mediated) HCO3- secretion, airway epithelia of newborns with cystic fibrosis (CF)…”
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Glucose depletion in the airway surface liquid is essential for sterility of the airways by Pezzulo, Alejandro A, Gutiérrez, Jeydith, Duschner, Kelly S, McConnell, Kelly S, Taft, Peter J, Ernst, Sarah E, Yahr, Timothy L, Rahmouni, Kamal, Klesney-Tait, Julia, Stoltz, David A, Zabner, Joseph

“…Diabetes mellitus predisposes the host to bacterial infections. Moreover, hyperglycemia has been shown to be an independent risk factor for respiratory…”
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Vitamin D-mediated effects on airway innate immunity in vitro by Stapleton, Emma M, Keck, Kathy, Windisch, Robert, Stroik, Mallory R, Thurman, Andrew L, Zabner, Joseph, Thornell, Ian M, Pezzulo, Alejandro A, Klesney-Tait, Julia, Comellas, Alejandro P

“…Vitamin D supplementation has been suggested to enhance immunity during respiratory infection season. We tested the effect of active vitamin D (calcitriol)…”
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Eosinophil expression of triggering receptor expressed on myeloid cells 1 (TREM-1) restricts type 2 lung inflammation by Bowen, Jayden L, Keck, Kathy, Baruah, Sankar, Nguyen, Kathy H, Thurman, Andrew L, Pezzulo, Alejandro A, Klesney-Tait, Julia

“…Asthma affects 25 million Americans, and recent advances in treatment are effective for only a portion of severe asthma patients. TREM-1, an innate receptor…”
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FXYD3 facilitates Na + and liquid absorption across human airway epithelia by increasing the transport capacity of the Na/K ATPase by Cano Portillo, Camilo, Villacreses, Raul, Thurman, Andrew L, Pezzulo, Alejandro A, Zabner, Joseph, Thornell, Ian M

“…Na/K ATPase activity is essential for ion transport across epithelia. FXYD3, a γ subunit of the Na/K ATPase, is expressed in the airway, but its function…”
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Increased ENaC-mediated liquid absorption across vitamin-D deficient human airway epithelia by Stapleton, Emma M, Thurman, Andrew L, Pezzulo, Alejandro A, Comellas, Alejandro P, Thornell, Ian M

“…Vitamin D deficiency is a risk factor for exacerbation of obstructive airway disease, a hallmark of which is mucus dehydration and plugging. Calcitriol (the…”
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Polarized AAVR expression determines infectivity by AAV gene therapy vectors by Hamilton, Bradley A., Li, Xiaopeng, Pezzulo, Alejandro A., Abou Alaiwa, Mahmoud H., Zabner, Joseph

“…Adeno-associated virus (AAV) has been investigated to transfer the cystic fibrosis transmembrane conductance regulator ( CFTR ) to airways. Inhaled AAV2- CFTR…”
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HSP90 inhibitor geldanamycin reverts IL-13- and IL-17-induced airway goblet cell metaplasia by Pezzulo, Alejandro A, Tudas, Rosarie A, Stewart, Carley G, Buonfiglio, Luis G Vargas, Lindsay, Brian D, Taft, Peter J, Gansemer, Nicholas D, Zabner, Joseph

“…Goblet cell metaplasia, a disabling hallmark of chronic lung disease, lacks curative treatments at present. To identify novel therapeutic targets for goblet…”
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Performance of a scalable RNA extraction-free transcriptome profiling method for adherent cultured human cells by Ghimire, Shreya, Stewart, Carley G., Thurman, Andrew L., Pezzulo, Alejandro A.

“…RNA sequencing enables high-content/high-complexity measurements in small molecule screens. Whereas the costs of DNA sequencing and RNA-seq library preparation…”
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Il-13 induces loss of CFTR in ionocytes and reduces airway epithelial fluid absorption by Romano Ibarra, Guillermo S, Lei, Lei, Yu, Wenjie, Thurman, Andrew L, Gansemer, Nicholas D, Meyerholz, David K, Pezzulo, Alejandro A, McCray, Paul B, Thornell, Ian M, Stoltz, David A

“…The airway surface liquid (ASL) plays a crucial role in lung defense mechanisms, and its composition and volume are regulated by the airway epithelium. The…”
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Cellular and molecular architecture of submucosal glands in wild-type and cystic fibrosis pigs by Yu, Wenjie, Moninger, Thomas O, Thurman, Andrew L, Xie, Yuliang, Jain, Akansha, Zarei, Keyan, Powers, Linda S, Pezzulo, Alejandro A, Stoltz, David A, Welsh, Michael J

“…Submucosal glands (SMGs) protect lungs but can also contribute to disease. For example, in cystic fibrosis (CF), SMGs produce abnormal mucus that disrupts…”
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TNFα and IL-17 alkalinize airway surface liquid through CFTR and pendrin by Rehman, Tayyab, Thornell, Ian M, Pezzulo, Alejandro A, Thurman, Andrew L, Romano Ibarra, Guillermo S, Karp, Philip H, Tan, Ping, Duffey, Michael E, Welsh, Michael J

“…The pH of airway surface liquid (ASL) is a key factor that determines respiratory host defense; ASL acidification impairs and alkalinization enhances key…”
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