Search Results - "Pettigrew, Donald W."

Functional and Metabolic Effects of Adaptive Glycerol Kinase (GLPK) Mutants in Escherichia coli by Applebee, M. Kenyon, Joyce, Andrew R., Conrad, Tom M., Pettigrew, Donald W., Palsson, Bernhard Ø.

“…Herein we measure the effect of four adaptive non-synonymous mutations to the glycerol kinase (glpK) gene on catalytic function and regulation, to identify…”
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Linkage between Fructose 1,6-Bisphosphate Binding and the Dimer−Tetramer Equilibrium of Escherichia coli Glycerol Kinase:  Critical Behavior Arising from Change of Ligand Stoichiometry by Yu, Peng, Pettigrew, Donald W

“…Escherichia coli glycerol kinase (EC 2.7.1.30; ATP−glycerol 3-phosphotransferase) is inhibited allosterically by fructose 1,6-bisphosphate (FBP), and this…”
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IIAGlc Inhibition of Glycerol Kinase:  A Communications Network Tunes Protein Motions at the Allosteric Site by Yu, Peng, Lasagna, Mauricio, Pawlyk, Aaron C., Reinhart, Gregory D., Pettigrew, Donald W.

“…Steady-state and time-resolved fluorescence anisotropy methods applied to an extrinsic fluorophore that is conjugated to non-native cysteine residues…”
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Transplanting Allosteric Control of Enzyme Activity by Protein-Protein Interactions: Coupling a Regulatory Site to the Conserved Catalytic Core by Pawlyk, Aaron C., Pettigrew, Donald W.

“…Glycerol kinase from Escherichia coli, but not Haemophilus influenzae, is inhibited allosterically by phosphotransferase system protein IIAGlc. The primary…”
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Oligomeric interactions provide alternatives to direct steric modes of control of sugar kinase/actin/hsp70 superfamily functions by heterotropic allosteric effectors: Inhibition of E. coli glycerol kinase by Pettigrew, Donald W.

“…Unlike those for monomeric superfamily members, heterotropic allosteric effectors of the tetrameric Escherichia coli glycerol kinase (EGK) bind to only one of…”
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Amino acid substitutions in the sugar kinase/hsp70/actin superfamily conserved ATPase core of E. coli glycerol kinase modulate allosteric ligand affinity but do not alter allosteric coupling by Pettigrew, Donald W.

“…IIA Glc, the glucose-specific phosphocarrier protein of the phospho enolpyruvate:glycose phosphotransferase system, is an allosteric inhibitor of Escherichia…”
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IIAGlc Allosteric Control of Escherichia coli Glycerol Kinase:  Binding Site Cooperative Transitions and Cation-Promoted Association by Zinc(II) by Holtman, C. Kay, Pawlyk, Aaron C, Meadow, Norman, Roseman, Saul, Pettigrew, Donald W

“…The catalytic activity of glycerol kinase (EC 2.7.1.30, ATP:glycerol 3-phosphotransferase) from Escherichia coli is inhibited allosterically by IIAGlc…”
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Reverse genetics of Escherichia coli glycerol kinase allosteric regulation and glucose control of glycerol utilization in vivo by Holtman, C K, Pawlyk, A C, Meadow, N D, Pettigrew, D W

“…Reverse genetics is used to evaluate the roles in vivo of allosteric regulation of Escherichia coli glycerol kinase by the glucose-specific phosphocarrier of…”
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Nucleotide regulation of Escherichia coli glycerol kinase: initial-velocity and substrate binding studies by Pettigrew, Donald W, Yu, Gui Ju, Liu, Youguo

“…Substrate binding to Escherichia coli glycerol kinase (EC 2.7.1.30; ATP-glycerol 3-phosphotransferase) was investigated by using both kinetics and binding…”
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The Cyclization of Farnesyl Diphosphate and Nerolidyl Diphosphate by a Purified Recombinant δ-Cadinene Synthase by Chauncey R. Benedict, Jia-Ling Lu, Pettigrew, Donald W., Jinggao Liu, Stipanovic, Robert D., Williams, Howard J.

“…The first step in the conversion of the isoprenoid intermediate, farnesyl diphosphate (FDP), to sesquiterpene phytoalexins in cotton (Gossypium barbadense)…”
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Crystal Structures of Escherichia coli Glycerol Kinase Variant S58→W in Complex with Nonhydrolyzable ATP Analogues Reveal a Putative Active Conformation of the Enzyme as a Result of Domain Motion by Bystrom, Cory E, Pettigrew, Donald W, Branchaud, Bruce P, O'Brien, Patrick, Remington, S. James

“…Escherichia coli glycerol kinase (GK) displays “half-of-the-sites” reactivity toward ATP and allosteric regulation by fructose 1,6-bisphosphate (FBP), which…”
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Glycerol kinase from Escherichia coli and an Ala65→Thr mutant: the crystal structures reveal conformational changes with implications for allosteric regulation by Feese, Michael D, Faber, H Rick, Bystrom, Cory E, Pettigrew, Donald W, Remington, S James

“…Background: Glycerol kinase (GK) from Escherichia coli is a velocity-modulated (V system) enzyme that has three allosteric effectors with independent…”
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Inactivation of Escherichia coli glycerol kinase by 5,5'-dithiobis(2-nitrobenzoic acid) and N-ethylmaleimide: evidence for nucleotide regulatory binding sites by Pettigrew, Donald W

“…Glycerol kinase (EC 2.7.1.30, ATP:glycerol 3-phosphotransferase) from Escherichia coli is inactivated by 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and by…”
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Escherichia coli Glycerol Kinase: Role of a Tetramer Interface in Regulation by Fructose 1,6-Bisphosphate and Phosphotransferase System Regulatory Protein IIIglc by Liu, Wei Zhang, Faber, Rick, Feese, Michael, Remington, S. James, Pettigrew, Donald W

“…Escherichia coli glycerol kinase (EC 2.7.1.30; ATP:glycerol 3-phosphotransferase) is a key element in a signal transduction pathway that couples expression of…”
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Unexpected presence of defective glpR alleles in various strains of Escherichia coli by Holtman, C K, Thurlkill, R, Pettigrew, D W

“…Alleles of glpR associated with the same GlpR(-) phenotype produce substitutions in different conserved portions of the glycerol 3-phosphate repressor which…”
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Conserved Active Site Aspartates and Domain–Domain Interactions in Regulatory Properties of the Sugar Kinase Superfamily by Pettigrew, Donald W., Smith, Gayle B., Thomas, Kimberly P., Dodds, D'Nette C.

“…The structures of the sugar kinase/heat shock 70/actin superfamily of enzymes show that the active site is located in a deep cleft between two domains whose…”
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A single amino acid change in Escherichia coli glycerol kinase abolishes glucose control of glycerol utilization in vivo by Pettigrew, D W, Liu, W Z, Holmes, C, Meadow, N D, Roseman, S

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Cation-Promoted Association of a Regulatory and Target Protein is Controlled by Protein Phosphorylation by Feese, Michael, Pettigrew, Donald W., Meadow, Norman D., Roseman, Saul, Remington, S. James

“…A central question in molecular biology concerns the means by which a regulatory protein recognizes different targets. IIIGlc, the glucose-specific…”
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Inactivation of Escherichia coli glycerol kinase by 5'-[p-(fluorosulfonyl)benzoyl]adenosine: protection by the hydrolyzed reagent by Pettigrew, Donald W

“…Incubation of Escherichia coli glycerol kinase (EC 2.7.1.30; ATP:glycerol 3-phosphotransferase) with 5'-[p-(fluorosulfonyl)benzoyl]adenosine (FSO2BzAdo) at pH…”
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IIA Glc Inhibition of Glycerol Kinase:  A Communications Network Tunes Protein Motions at the Allosteric Site by Yu, Peng, Lasagna, Mauricio, Pawlyk, Aaron C., Reinhart, Gregory D., Pettigrew, Donald W.

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