Differential expression of the non-receptor tyrosine kinase BRK in oral squamous cell carcinoma and normal oral epithelium

BRK is a non-receptor tyrosine kinase whose functional role is poorly understood. Although it is an epithelial specific kinase, its expression appears to be tissue specific. To date, little is known about BRK expression in human oral epithelium. We investigated expression of BRK in human oral squamo...

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Bibliographic Details
Published in:	Oral oncology Vol. 40; no. 10; pp. 1040 - 1047
Main Authors:	Petro, B.J., Tan, R.C., Tyner, A.L., Lingen, M.W., Watanabe, K.
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 01-11-2004 Elsevier
Subjects:	Biological and medical sciences Blotting, Western BRK Carcinoma, Squamous Cell - enzymology Humans Medical sciences Microscopy, Confocal Mouth Mucosa - enzymology Mouth Neoplasms - enzymology Neoplasm Proteins Non-receptor tyrosine kinase Oral squamous cell carcinoma Otorhinolaryngology. Stomatology Protein-Tyrosine Kinases - metabolism PTK6 Sik Tumor Cells, Cultured Tumors BRK PTK6 Oral squamous cell carcinoma Sik Non-receptor tyrosine kinase Squamous cell carcinoma Enzyme Stomatology Transferases ENT Malignant tumor Epithelium Cancerology Protein-tyrosine kinase Biological receptor
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Summary:	BRK is a non-receptor tyrosine kinase whose functional role is poorly understood. Although it is an epithelial specific kinase, its expression appears to be tissue specific. To date, little is known about BRK expression in human oral epithelium. We investigated expression of BRK in human oral squamous cell carcinomas (OSCC) and normal oral epithelium (NOE) using immunohistochemistry, laser confocal microscopy and Western blotting. The subcellular localization of BRK was identified by confocal microscopy and Western blotting of nuclear and cytoplasmic extracts from these cells. The results indicate that NOE express higher levels of BRK compared with OSCC cells. In NOE and moderately differentiated OSCC cells, BRK was localized in the nucleus and cytoplasm. However, in poorly differentiated OSCC cells, BRK was localized in perinuclear regions. These results suggest that BRK expression differs in normal and OSCC which may reflect a possible functional involvement in OSCC.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1368-8375 1879-0593
DOI:	10.1016/j.oraloncology.2004.05.010