The Use of Polyvinyl Alcohol Glutaraldehyde as Solid-phase in ELISA for Plague

The Use of Polyvinyl Alcohol Glutaraldehyde as Solid-phase in ELISA for Plague

Discs of polyvinyl alcohol cross-linked with glutaraldehyde were synthesized under acid catalysis (H2SO4). Then, the antigen F1 purified from Yersinia pestis was covalently linked to this modified polymer. Afterwards, an enzyme-linked immunosorbent assay (ELISA) was established for the diagnosis of...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz Vol. 91; no. 2; pp. 195 - 198
Main Authors: Araujo, Aureci Maria, Petribú, André Tavares S, Barbosa, Gustavo Henrique T Sales, Diniz, José Ricardo P, Almeida, Alzira MP de, Azevedo, Walter Mendes, Malagueño, Elizabeth, Carvalho Jr, Luiz B
Format: Journal Article
Language:English
Published: Brazil Fundação Oswaldo Cruz, Fiocruz 01-04-1996
Instituto Oswaldo Cruz, Ministério da Saúde
Fundação Oswaldo Cruz (FIOCRUZ)
Subjects:
Animals
Brazil
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Glutaral
glutaraldehyde
Humans
PARASITOLOGY
Plague - diagnosis
Polyvinyl Alcohol
polyvinyl alcohol glutaraldehyde Yersinia pestis ELISA Brazil
Rabbits
TROPICAL MEDICINE
Yersinia pestis
Brazil
glutaraldehyde
polyvinyl alcohol
Yersinia pestis
ELISA
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Summary:Discs of polyvinyl alcohol cross-linked with glutaraldehyde were synthesized under acid catalysis (H2SO4). Then, the antigen F1 purified from Yersinia pestis was covalently linked to this modified polymer. Afterwards, an enzyme-linked immunosorbent assay (ELISA) was established for the diagnosis of plague in rabbit and human. The best conditions for the method were achieved by using 1.3 ug of F1 prepared in 0.067 M phosphate buffer, pH 7.2, containing 1 M NaCl (PBS); anti-IgG peroxidase conjugate diluted 6,000 times and as a blocking agent 3% w/v skim milk in PBS. The titration of positive rabbit serum according to this procedure detected antibody concentrations up to 1:12,800 times. The present method, the conventional ELISA and passive haemagglutination assay are compared.
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ISSN:1678-8060
0074-0276
0074-0276
1678-8060
DOI:10.1590/S0074-02761996000200014