Kinetics and equilibria of condensation reactions between monosaccharide pairs catalyzed by Aspergillus niger glucoamylase

Arabinose, fructose, galactose, myo-inositol, lyxose, mannose, ribose, and xylose were incubated individually and with glucose in the presence of Aspergillus niger glucoamylase at pH 4.5 and 45 degrees C. Glucoamylase condenses galactose, glucose, and mannose individually into disaccharides. It also...

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Bibliographic Details
Published in:	Biotechnology and bioengineering Vol. 56; no. 1; pp. 9 - 22
Main Authors:	Pestlin, S, Prinz, D, Starr, J.N, Reilly, P.J
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 05-10-1997 Wiley
Subjects:	Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology condensation reactions disaccharides equilibria Fundamental and applied biological sciences. Psychology glucoamylase kinetics Methods. Procedures. Technologies monosaccharides plant biochemistry plant physiology Ose Glucan 1,4-α-glucosidase Equilibrium constant Enzyme Condensation Disaccharide Fungi Aspergillus niger Glycosidases Hydrolases Carbohydrate Fungi Imperfecti Kinetics Thallophyta Enzymatic reaction
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Summary:	Arabinose, fructose, galactose, myo-inositol, lyxose, mannose, ribose, and xylose were incubated individually and with glucose in the presence of Aspergillus niger glucoamylase at pH 4.5 and 45 degrees C. Glucoamylase condenses galactose, glucose, and mannose individually into disaccharides. It also produces mixed disaccharides when each of the eight carbohydrates is incubated with glucose. Many products were identified by gas chromatography of the derivatized reaction mixtures followed by mass spectroscopy of the individual chromatographic peaks. Galacto, gluco-, or mannopyranosyl rings appear to be present at the nonreducing ends of all the disaccharides produced. Molecules linked through primary hydroxyl groups have the highest equilibrium constants of all products formed, since these bonds are thermodynamically favored. However, glucoamylase is capable of forming bonds with many available hydroxyl groups, as previously demonstrated when it was incubated with glucose alone. Formation rates of different bonds linking different residues vary widely. These results demonstrate that glucoamylase has a wide selectivity toward residues it will condense into disaccharides and toward bonds it will form between them.
Bibliography:	ArticleID:BIT2 istex:8F51F00B34DA8E22B6EEC2474E631FBD22B691D9 ark:/67375/WNG-W4GVLJF7-0 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0006-3592 1097-0290
DOI:	10.1002/(SICI)1097-0290(19971005)56:1<9::AID-BIT2>3.0.CO;2-O