Characterization of Native and Human Serum Albumin-Bound Lysophosphatidic Acid Species and Their Effect on the Viability of Mesenchymal Stem Cells In Vitro

Characterization of Native and Human Serum Albumin-Bound Lysophosphatidic Acid Species and Their Effect on the Viability of Mesenchymal Stem Cells In Vitro

Scaffolds can provide a healthy environment for cell attachment, differentiation, proliferation, and migration in vitro and in vivo. Lysophosphatidic acid (LPA) is a naturally occurring bioactive phospholipid that is present in the serum mainly bound to albumin. The present study aims to investigate...

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Bibliographic Details
Published in:Applied sciences Vol. 12; no. 16; p. 8183
Main Authors: Majer, Aliz, Pesthy, Julianna, Besztercei, Balázs, Hinsenkamp, Adél, Smeller, László, Lacza, Zsombor, Benyó, Zoltán, Ruisanchez, Éva, Hornyák, István
Format: Journal Article
Language:English
Published: Basel MDPI AG 01-08-2022
Subjects:
Albumin
Binding sites
Biocompatibility
Bone growth
Bone marrow
Bones
Cell adhesion
Cell differentiation
Cell growth
Cell proliferation
Cell viability
Complex formation
Cytotoxicity
FTIR
Human serum albumin
Infrared spectroscopy
Lipids
Lysophosphatidic acid
Mesenchymal stem cells
Normal distribution
Phospholipids
Regeneration
Regeneration (physiology)
Regenerative medicine
scaffold
Scaffolds
Serum albumin
Software
Spectrum analysis
Statistical analysis
Stem cells
Tissue engineering
viability
Wound healing
St Louis Missouri
United States > US
Japan
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Summary:Scaffolds can provide a healthy environment for cell attachment, differentiation, proliferation, and migration in vitro and in vivo. Lysophosphatidic acid (LPA) is a naturally occurring bioactive phospholipid that is present in the serum mainly bound to albumin. The present study aims to investigate the biocompatibility of LPA. It also aims to determine the effect of different LPA species on the proliferation and migration of human bone marrow-derived mesenchymal stem cells (hBM-dMSCs) for LPA and human serum albumin (HSA) containing bone scaffold development. The HSA-LPA complex formation was assessed using Fourier-transform infrared (FTIR) spectroscopy. The effect of 18:1, 18:2, or 16:0 LPA alone, or in combination with 4% HSA, on cell viability and proliferation was determined by XTT. The cell migration was examined in a wound healing assay. The changes in the FTIR spectra of LPA-HSA compositions, compared with HSA alone, indicate the complex formation between the components. Our study showed that 18:1, 18:2, and 16:0 LPA species had no cytotoxic effects up to 10 µM concentration. The different LPA species increased the proliferation of hBM-dMSCs in a dose-dependent manner when administered in the presence of HSA, without an effect on the migration of this cell type. These findings make the in vivo application of LPA-HSA complex promising for bone regeneration.
ISSN:2076-3417
2076-3417
DOI:10.3390/app12168183