Search Results - "Persechini, Anthony"

In Calmodulin–IQ Domain Complexes, the Ca2+-Free and Ca2+-Bound Forms of the Calmodulin C-Lobe Direct the N-Lobe to Different Binding Sites by Black, D. J, Persechini, Anthony

“…We have investigated the roles played by the calmodulin (CaM) N- and C-lobes in establishing the conformations of CaM–IQ domain complexes in different…”
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Effects of Combined Phosphorylation at Ser-617 and Ser-1179 in Endothelial Nitric-oxide Synthase on EC50(Ca2+) Values for Calmodulin Binding and Enzyme Activation by Tran, Quang-Kim, Leonard, Jared, Black, D.J., Nadeau, Owen W., Boulatnikov, Igor G., Persechini, Anthony

“…We have investigated the possible biochemical basis for enhancements in NO production in endothelial cells that have been correlated with agonist- or shear…”
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The Relationship between the Free Concentrations of Ca2+ and Ca2+-calmodulin in Intact Cells by Persechini, Anthony, Cronk, Benjamin

“…Using stably expressed fluorescent indicator proteins, we have determined for the first time the relationship between the free Ca2+ and Ca2+-calmodulin…”
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Phosphorylation within an Autoinhibitory Domain in Endothelial Nitric Oxide Synthase Reduces the Ca2+ Concentrations Required for Calmodulin To Bind and Activate the Enzyme by Tran, Quang-Kim, Leonard, Jared, Black, D. J, Persechini, Anthony

“…We have investigated the effects of phosphorylation at Ser-617 and Ser-635 within an autoinhibitory domain (residues 595−639) in bovine endothelial nitric…”
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Monitoring the total available calmodulin concentration in intact cells over the physiological range in free Ca2 by Black, D J, Tran, Quang-Kim, Persechini, Anthony

“…We describe the design, characterization and application of a new genetically encoded fluorescent biosensor for intracellular detection of both free…”
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Real-Time Evaluation of Myosin Light Chain Kinase Activation in Smooth Muscle Tissues from a Transgenic Calmodulin-Biosensor Mouse by Isotani, Eiji, Zhi, Gang, Lau, Kim S., Huang, Jian, Mizuno, Yusuke, Persechini, Anthony, Geguchadze, Ramaz, Kamm, Kristine E., Stull, James T., Korn, Edward D.

“…Ca2+/calmodulin (CaM)-dependent phosphorylation of myosin regulatory light chain (RLC) by myosin light chain kinase (MLCK) initiates smooth muscle contraction…”
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Intracellular Coupling via Limiting Calmodulin by Tran, Quang-Kim, Black, D J, Persechini, Anthony

“…Measurements of cellular Ca 2 + -calmodulin concentrations have suggested that competition for limiting calmodulin may couple calmodulin-dependent activities…”
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Dynamic changes in free Ca-calmodulin levels in adult cardiac myocytes by Maier, Lars S., Ziolo, Mark T., Bossuyt, Julie, Persechini, Anthony, Mestril, Ruben, Bers, Donald M.

“…Despite its multifunctional role in cardiac myocyte function, little is known about dynamic changes in activation state of calmodulin (CaM). Thus, the purpose…”
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The Kinetics of Ca2+-Dependent Switching in a Calmodulin−IQ Domain Complex by Black, D. J, Selfridge, J. Eva, Persechini, Anthony

“…We have performed a kinetic analysis of Ca2+-dependent switching in the complex between calmodulin (CaM) and the IQ domain from neuromodulin, and have…”
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Calmodulin Directly Gates Gap Junction Channels by Peracchia, C, Sotkis, A, Wang, X G, Peracchia, L L, Persechini, A

“…Cytosolic changes control gap junction channel gating via poorly understood mechanisms. In the past two decades calmodulin participation in gating has been…”
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Lobe-dependent Regulation of Ryanodine Receptor Type 1 by Calmodulin by Xiong, Liang-Wen, Newman, Rhonda A, Rodney, George G, Thomas, Oluwatoyin, Zhang, Jia-Zheng, Persechini, Anthony, Shea, Madeline A, Hamilton, Susan L

“…Calmodulin activates the skeletal muscle Ca 2+ release channel RYR1 at n m Ca 2+ concentrations and inhibits the channel at μ m Ca 2+ concentrations. Using a…”
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Investigating the landscape of intracellular [Ca2+] in live cells by rapid photoactivated cross-linking of calmodulin-protein interactions by Persechini, Anthony, Armbruster, Hailey, Keightley, Andrew

“…•We have developed a method for rapid capture of calmodulin protein interactions as they occur throughout a living cell. Captured proteins are affinity…”
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Different Mechanisms for Ca Dissociation from Complexes of Calmodulin with Nitric Oxide Synthase or Myosin Light Chain Kinase by Persechini, Anthony, White, Howard D., Gansz, Krista J.

“…We have determined the stoichiometry and rate constants for the dissociation of Ca ion from calmodulin (CaM) complexed with rabbit skeletal muscle myosin light…”
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Ca2+ Binding and Energy Coupling in the Calmodulin-Myosin Light Chain Kinase Complex by Persechini, Anthony, Yano, Kenji, Stemmer, Paul M.

“…We have previously shown that 3 Ca2+ ions are released cooperatively and 1 independently from the complex between (Ca2+)4-calmodulin and skeletal muscle myosin…”
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Activation of Myosin Light Chain Kinase Requires Translocation of Bound Calmodulin by Krueger, Joanna K., Gallagher, Stephen C., Zhi, Gang, Geguchadze, Ramaz, Persechini, Anthony, Stull, James T., Trewhella, Jill

“…A novel translocation step is inferred from structural studies of the interactions between the intracellular calcium receptor protein calmodulin (CaM) and one…”
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The EF-hand family of calcium-modulated proteins by Persechini, A, Moncrief, N D, Kretsinger, R H

“…The EF-hand homolog proteins bind calcium (Ca2+) with dissociation constants in the micromolar range and are modulated by stimulus-induced increases in…”
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Evaluating Calmodulin–Protein Interactions by Rapid Photoactivated Cross-Linking in Live Cells Metabolically Labeled with Photo-Methionine by Black, DJ, Tran, Quang-Kim, Keightley, Andrew, Chinawalkar, Ameya, McMullin, Cole, Persechini, Anthony

“…This work addresses the question of how the Ca2+ sensor protein calmodulin shapes cellular responses to Ca2+ signals. Proteins interacting with affinity tagged…”
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Localization of Unique Functional Determinants in the Calmodulin Lobes to Individual EF Hands by Persechini, Anthony, Stemmer, Paul M., Ohashi, Ichiro

“…We have investigated the functional interchangeability of EF hands I and III or II and IV, which occupy structurally analogous positions in the native I-II and…”
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In Calmodulin–IQ Domain Complexes, the Ca 2+ -Free and Ca 2+ -Bound Forms of the Calmodulin C-Lobe Direct the N-Lobe to Different Binding Sites by Black, D. J., Persechini, Anthony

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In Calmodulin-IQ Domain Complexes, the Ca super(2+)-Free and Ca super(2+)-Bound Forms of the Calmodulin C-Lobe Direct the N-Lobe to Different Binding Sites by Black, D J, Persechini, Anthony

“…We have investigated the roles played by the calmodulin (CaM) N- and C-lobes in establishing the conformations of CaM-IQ domain complexes in different Ca…”
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