Search Results - "Perret, Sylvie"

A CHO stable pool production platform for rapid clinical development of trimeric SARS‐CoV‐2 spike subunit vaccine antigens by Joubert, Simon, Stuible, Matthew, Lord‐Dufour, Simon, Lamoureux, Linda, Vaillancourt, François, Perret, Sylvie, Ouimet, Manon, Pelletier, Alex, Bisson, Louis, Mahimkar, Rohan, Pham, Phuong Lan, L′Ecuyer‐Coelho, Helene, Roy, Marjolaine, Voyer, Robert, Baardsnes, Jason, Sauvageau, Janelle, St‐Michael, Frank, Robotham, Anna, Kelly, John, Acel, Andrea, Schrag, Joseph D., El Bakkouri, Majida, Durocher, Yves

“…Protein expression from stably transfected Chinese hamster ovary (CHO) clones is an established but time‐consuming method for manufacturing therapeutic…”
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A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications by Raymond, Céline, Tom, Roseanne, Perret, Sylvie, Moussouami, Pascal, L’Abbé, Denis, St-Laurent, Gilles, Durocher, Yves

“…Transient gene expression in mammalian cells is a valuable alternative to stable cell lines for the rapid production of large amounts of recombinant proteins…”
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Simplifying glycan monitoring of complex antigens such as the SARS-CoV-2 spike to accelerate vaccine development by Sauvageau, Janelle, Koyuturk, Izel, St. Michael, Frank, Brochu, Denis, Goneau, Marie-France, Schoenhofen, Ian, Perret, Sylvie, Star, Alexandra, Robotham, Anna, Haqqani, Arsalan, Kelly, John, Gilbert, Michel, Durocher, Yves

“…Glycosylation is a key quality attribute that must be closely monitored for protein therapeutics. Established assays such as HILIC-Fld of released glycans and…”
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Arsenal of nanobodies shows broad-spectrum neutralization against SARS-CoV-2 variants of concern in vitro and in vivo in hamster models by Rossotti, Martin A., van Faassen, Henk, Tran, Anh T., Sheff, Joey, Sandhu, Jagdeep K., Duque, Diana, Hewitt, Melissa, Wen, Xiaoxue, Bavananthasivam, Jegarubee, Beitari, Saina, Matte, Kevin, Laroche, Geneviève, Giguère, Patrick M., Gervais, Christian, Stuible, Matthew, Guimond, Julie, Perret, Sylvie, Hussack, Greg, Langlois, Marc-André, Durocher, Yves, Tanha, Jamshid

“…Nanobodies offer several potential advantages over mAbs for the control of SARS-CoV-2. Their ability to access cryptic epitopes conserved across SARS-CoV-2…”
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Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells by Loignon, Martin, Perret, Sylvie, Kelly, John, Boulais, Denise, Cass, Brian, Bisson, Louis, Afkhamizarreh, Fatemeh, Durocher, Yves

“…Mammalian cells are becoming the prevailing expression system for the production of recombinant proteins because of their capacity for proper protein folding,…”
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Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids by Mignaqui, Ana Clara, Ruiz, Vanesa, Perret, Sylvie, St-Laurent, Gilles, Singh Chahal, Parminder, Transfiguracion, Julia, Sammarruco, Ayelén, Gnazzo, Victoria, Durocher, Yves, Wigdorovitz, Andrés

“…Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently…”
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Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: Peptone additives improve cell growth and transfection efficiency by Pham, Phuong Lan, Perret, Sylvie, Doan, Huyen Chau, Cass, Brian, St-Laurent, Gilles, Kamen, Amine, Durocher, Yves

“…Large‐scale transient transfection of mammalian cells is a recent and powerful technology for the fast production of milligram amounts of recombinant proteins…”
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A Reporter Gene Assay for High-Throughput Screening of G-Protein-Coupled Receptors Stably or Transiently Expressed in HEK293 EBNA Cells Grown in Suspension Culture by Durocher, Yves, Perret, Sylvie, Thibaudeau, Eric, Gaumond, Marie-Helene, Kamen, Amine, Stocco, Rino, Abramovitz, Mark

“…We describe in detail a robust, sensitive, and versatile functional assay for G-protein-coupled receptors (GPCRs) expressed in human embryonic kidney (HEK)…”
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Rapid protein production from stable CHO cell pools using plasmid vector and the cumate gene-switch by Poulain, Adeline, Perret, Sylvie, Malenfant, Félix, Mullick, Alaka, Massie, Bernard, Durocher, Yves

“…•Optimal parameters for generating stable inducible CHO pools expressing recombinant proteins were identified.•The CHO stable pool generation process is robust…”
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Rapid, high-yield production of full-length SARS-CoV-2 spike ectodomain by transient gene expression in CHO cells by Stuible, Matthew, Gervais, Christian, Lord-Dufour, Simon, Perret, Sylvie, L’Abbé, Denis, Schrag, Joseph, St-Laurent, Gilles, Durocher, Yves

“…•CHO cells are more efficient than 293 cells for expressing trimeric SARS-CoV-2 spike protein.•A trimerization domain and elimination of the S1/S2 furin site…”
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On-line monitoring of respiration in recombinant-baculovirus infected and uninfected insect cell bioreactor cultures by Kamen, Amine A., Bédard, Charles, Tom, Rosanne, Perret, Sylvie, Jardin, Barbara

“…Respiration rates in Spodoptera frugiperda (Sf‐9) cell bioreactor cultures were successfully measured on‐line using two methods: The O2 uptake rate (OUR) was…”
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Optimization of a high-cell-density polyethylenimine transfection method for rapid protein production in CHO-EBNA1 cells by Stuible, Matthew, Burlacu, Alina, Perret, Sylvie, Brochu, Denis, Paul-Roc, Béatrice, Baardsnes, Jason, Loignon, Martin, Grazzini, Eric, Durocher, Yves

“…•EBNA1 expression in CHO cells improves transient gene expression from OriP-containing plasmid vectors.•A new, transfection-compatible media formulation…”
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High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells by Durocher, Yves, Perret, Sylvie, Kamen, Amine

“…A scalable transfection procedure using polyethylenimine (PEI) is described for the human embryonic kidney 293 cell line grown in suspension. Green fluorescent…”
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Production of afucosylated antibodies in CHO cells by coexpression of an anti‐FUT8 intrabody by Joubert, Simon, Guimond, Julie, Perret, Sylvie, Malenfant, Félix, Elahi, S. Mehdy, Marcil, Anne, Parat, Marie, Gilbert, Michel, Lenferink, Anne E.G., Baardsnes, Jason, Durocher, Yves

“…Some effector functions prompted by immunoglobulin G (IgG) antibodies, such as antibody‐dependent cell‐mediated cytotoxicity (ADCC), strongly depend on the…”
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Recombinant Protein Production from Stable CHO Cell Pools by Delafosse, Laurence, Lord-Dufour, Simon, Pelletier, Alex, Perret, Sylvie, Burlacu, Alina, Ouimet, Manon, Cass, Brian, Joubert, Simon, Stuible, Matthew, Durocher, Yves

“…The continuous improvement of expression platforms is necessary to respond to the increasing demand for recombinant proteins that are required to carry out…”
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Stable expression of chimeric heavy chain antibodies in CHO cells by Agrawal, Vishal, Slivac, Igor, Perret, Sylvie, Bisson, Louis, St-Laurent, Gilles, Murad, Yanal, Zhang, Jianbing, Durocher, Yves

“…Camelid single domain antibodies fused to noncamelid Fc regions, also called chimeric heavy chain antibodies (cHCAb), offer great potential as therapeutic and…”
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Transient Gene Expression in Serum-Free Suspension-Growing Mammalian Cells for the Production of Foot-and-Mouth Disease Virus Empty Capsids. e72800 by Mignaqui, Ana Clara, Ruiz, Vanesa, Perret, Sylvie, St-Laurent, Gilles, Chahal, Parminder Singh, Transfiguracion, Julia, Sammarruco, Ayelen, Gnazzo, Victoria, Durocher, Yves, Wigdorovitz, Andres

“…Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently…”
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Development of a suspension serum-free helper-dependent adenovirus production system and assessment of co-infection conditions by Meneses-Acosta, Angélica, Dormond, Edwige, Jacob, Danielle, Tom, Roseanne, Bernier, Alice, Perret, Sylvie, St-Laurent, Gilles, Durocher, Yves, Gilbert, Rénald, Kamen, Amine

“…Helper-dependent adenovirus (HDAd), deleted in all viral protein-coding sequences has been designed to reduce immune response and favor long-term expression of…”
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CAT II / OTS CAT II operations using existing CAT I Ground Based Augmentation System by Behrend, Ferdinand, Lehmann, Oliver, De Smedt, David, Grand-Perret, Sylvie

“…Flight tests performed in an A330 simulator have shown that the existing standardized Ground Based Augmentation System (GBAS) CAT I could be suitable for CAT…”
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Dissolved carbon dioxide accumulation in a large scale and high density production of TGFβ receptor with baculovirus infected Sf-9 cells by Garnier, A, Voyer, R, Tom, R, Perret, S, Jardin, B, Kamen, A

“…Production of a TGFβ receptor with high density baculovirus infected Sf-9 cells (7×10(6)cells ml(-1)) served as a test run for a retrofitted 150 L microbial…”
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