Draft genome of neotropical Bacillus thuringiensis UFT038 and its potential against lepidopteran soybean pests

Draft genome of neotropical Bacillus thuringiensis UFT038 and its potential against lepidopteran soybean pests

Bacillus thuringiensis ( Bt ) is known for its Cry and Vip3A pesticidal proteins with high selectivity to target pests. Here, we assessed the potential of a novel neotropical Bt strain (UFT038) against six lepidopteran pests, including two Cry-resistant populations of fall armyworm, Spodoptera frugi...

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Bibliographic Details
Published in:Folia microbiologica Vol. 69; no. 1; pp. 91 - 99
Main Authors: Alves, Giselly B., Lemes, Timóteo S. O., Pereira, Eliseu J. G., Jurat-Fuentes, Juan L., Smagghe, Guy, Santos, Gil R., Haddi, Khalid, Corrêa, Roberto F. T., Melo, Fernando L., Jumbo, Luis O. Viteri, Oliveira, Eugenio E., Peron, Antônio J., Ribeiro, Bergmann M., Aguiar, Raimundo W. S.
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-02-2024
Springer Nature B.V
Subjects:
Applied Microbiology
Bacillus thuringiensis
Bioassays
Biomedical and Life Sciences
Environmental Engineering/Biotechnology
Genes
Genomes
Immunology
Insecticide resistance
Larval development
Life Sciences
Microbiology
Original Article
Pests
Populations
Soybeans
Spodoptera frugiperda
Toxicology
Toxins
Virulence
Virulence factors
Genome sequencing, Cry proteins
resistance
Lepidoptera
Bt resistance
Bacillus thuringiensis
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Summary:Bacillus thuringiensis ( Bt ) is known for its Cry and Vip3A pesticidal proteins with high selectivity to target pests. Here, we assessed the potential of a novel neotropical Bt strain (UFT038) against six lepidopteran pests, including two Cry-resistant populations of fall armyworm, Spodoptera frugiperda . We also sequenced and analyzed the genome of Bt UFT038 to identify genes involved in insecticidal activities or encoding other virulence factors. In toxicological bioassays, Bt UFT038 killed and inhibited the neonate growth in a concentration-dependent manner.  Bt UFT038 and HD-1 were equally toxic against  S. cosmioides , S. frugiperda ( S_Bt and R_Cry1 + 2Ab populations), Helicoverpa zea , and H. armigera . However, larval growth inhibition results indicated that Bt UFT038 was more toxic than HD-1 to S. cosmioides , while HD-1 was more active against Chrysodeixis includens . The draft genome of Bt UFT038 showed the cry1Aa8 , cry1Ac11 , cry1Ia44 , cry2Aa9 , cry2Ab35 , and vip3Af5 genes. Besides this, genes encoding the virulence factors ( inhA , plcA , piplC , sph , and chi1-2 ) and toxins ( alo , cytK , hlyIII , hblA-D , and nheA-C ) were also identified. Collectively, our findings reveal the potential of the Bt UFT038 strain as a source of insecticidal genes against lepidopteran pests, including S. cosmioides and S. frugiperda .
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ISSN:0015-5632
1874-9356
DOI:10.1007/s12223-023-01114-3