Search Results - "Perler, F"

InBase: the Intein Database by Perler, Francine B

“…Inteins are self-catalytic protein splicing elements. InBase (http://www.neb.com/neb/inteins.html), the Intein Database and Registry, is a curated compilation…”
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Compilation and analysis of intein sequences by Perler, Francine B., Olsen, Gary J., Adam, Eric

“…We have compiled a list of all the inteins (protein splicing elements) whose sequences have been published or were available from on-line sequence databases as…”
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The mechanism of protein splicing and its modulation by mutation by Xu, M. Q., Perler, F. B.

“…Protein splicing results in the expression of two mature proteins from a single gene. After synthesis of a precursor protein, an internal segment (the intein)…”
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InBase, the Intein Database by Perler, F B

“…InBase, the Intein Database (http://www.neb.com/neb/inteins.html ), is a comprehensive on-line resource that includes the Intein Registry. Inteins are protein…”
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Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein by Southworth, M W, Amaya, K, Evans, T C, Xu, M Q, Perler, F B

“…The Mycobacterium xenopi gyrase A mini-intein has been engineered to yield a controllable N-terminal or C-terminal, single-splice-junction autocleavage…”
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An alternative protein splicing mechanism for inteins lacking an N-terminal nucleophile by Southworth, Maurice W., Benner, Jack, Perler, Francine B.

“…Variations in the intein‐mediated protein splicing mechanism are becoming more apparent as polymorphisms in conserved catalytic residues are identified. The…”
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Extremozymes: Expanding the Limits of Biocatalysis by Adams, Michael W.W, Perler, Francine B, Kelly, Robert M

“…The study of enzymes isolated from organisms inhabiting unconventional ecosystems has led to the realization that biocatalysis need not be constrained to mild…”
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Protein splicing elements: inteins and exteins — a definition of terms and recommended nomenclature by Perler, Francine B., Davis, Elaine O., Dean, Gary E., Gimble, Frederick S., Jack, William E., Neff, Norma, Noren, Christopher J., Thorner, Jeremy, Belfort, Marlene

“…Several archaeal, eubacterial and eucaryotic genes have been identified with in-frame insertions that are excised at the protein level, not at the RNA level…”
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Protein Splicing of Inteins and Hedgehog Autoproteolysis: Structure, Function, and Evolution by Perler, Francine B

“…Protein splicing is a posttranslational editing process that removes an internal protein fragment (intein) from a precursor and ligates the external protein…”
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InBase, the New England Biolabs Intein Database by Perler, F B

“…Inteins are intervening sequences that splice as proteins, not RNA. InBase, the New England Biolabs Intein Database (http://www.neb.com/neb/inteins.html), is a…”
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Rescue of protein splicing activity from a Magnetospirillum magnetotacticum intein-like element by Southworth, M W, Yin, J, Perler, F B

“…The self-catalytic protein splicing mechanism is mediated by the intein plus the first amino acid following the intein C-terminus (termed the +1 residue)…”
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In vitro protein splicing of purified precursor and the identification of a branched intermediate by Xu, M Q, Southworth, M W, Mersha, F B, Hornstra, L J, Perler, F B

“…Protein splicing is a posttranslational processing event in which an internal polypeptide is excised from a protein precursor and the terminal polypeptides are…”
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Cloning of Thermostable DNA Polymerases from Hyperthermophilic Marine Archaea with Emphasis on Thermococcus sp. 9 degrees N-7 and Mutations Affecting 3′-5′ Exonuclease Activity by Southworth, M. W., Kong, H., Kucera, R. B., Ware, J., Jannasch, H. W., Perler, F. B.

“…Five extremely thermophilic Archaea from hydrothermal vents were isolated, and their DNA polymerases were cloned and expressed in Escherichia coli. Protein…”
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Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element by Chong, Shaorong, Mersha, Fana B, Comb, Donald G, Scott, Melissa E, Landry, David, Vence, Luis M, Perler, Francine B, Benner, Jack, Kucera, Rebecca B, Hirvonen, Christine A, Pelletier, John J, Paulus, Henry, Xu, Ming-Qun

“…A novel protein purification system has been developed which enables purification of free recombinant proteins in a single chromatographic step. The system…”
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Intervening Sequences in an Archaea DNA Polymerase Gene by Perler, Francine B., Comb, Donald G., Jack, William E., Moran, Laurie S., Qiang, Boqin, Kucera, Rebecca B., Benner, Jack, Slatko, Barton E., Nwankwo, Donald O., Hempstead, S. Kay, Clotilde K. S. Carlow, Jannasch, Holger

“…The DNA polymerase gene from the Archaea Thermococcus litoralis has been cloned and expressed in Escherichia coli. It is split by two intervening sequences…”
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Protein splicing and its applications by Perler, Francine B, Adam, Eric

“…Protein splicing elements, termed inteins, provide a fertile source for innovative biotechnology tools. First harnessed for protein purification, inteins are…”
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Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system by Chong, S. (New England Biolabs, Beverly, MA.), Shao, Y, Paulus, H, Benner, J, Perler, F.B, Xu, M.W

“…Protein splicing involves the excision of an internal protein segment, the intein, from a precursor protein and the concomitant ligation of the flanking N- and…”
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Hyperthermophilic inteins by Perler, F B

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Protein splicing removes intervening sequences in an archaea DNA polymerase by Hodges, Robert A., Perler, Francine B., Noren, Christopher J., Jack, William E.

“…The Vent DNA polymerase gene from Thermococcus litorails contains two in-frame insertions that must be spliced out to form the mature polymerase. Primer…”
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Protein splicing and autoproteolysis mechanisms by Perler, Francine B, Xu, Ming-Qun, Paulus, Henry

“…It has generally been assumed that the conversion of all inactive protein precursors to biologically active proteins is mediated by specific processing…”
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