Genetic mapping of Uba3 O.2.2 , a pupal lethal mutation in Drosophila melanogaster
An EMS mutagenesis screen was conducted in Drosophila melanogaster to identify growth control mutants. The multi-institution Fly-CURE consortium phenotypically characterized the O.2.2 mutant using the FLP/FRT system which displayed a mutant lethal phenotype with reduced head development, and darkene...
Saved in:
Published in: | microPublication biology Vol. 2022 |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Format: | Journal Article |
Language: | English |
Published: |
United States
Caltech Library
2022
|
Subjects: | |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | An EMS mutagenesis screen was conducted in
Drosophila melanogaster
to identify growth control mutants. The multi-institution Fly-CURE consortium phenotypically characterized the
O.2.2
mutant using the
FLP/FRT
system which displayed a mutant lethal phenotype with reduced head development, and darkened ocular tissue. Complementation mapping was conducted to identify the affected gene. A failure to complement was identified in
Uba3
, resulting in the identification of the novel allele,
Uba3
O.2.2
.
Uba3
is a known disruptor of the cell cycle and our data are consistent with early larval/embryonic lethality displayed in numerous species. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 JA: Data curation, Formal analysis, Investigation, Writing - review & editing DP: Data curation, Formal analysis, Investigation, Writing - review & editing NP: Data curation, Formal analysis, Investigation, Writing - review & editing AB: Data curation, Formal analysis, Investigation, Writing - review & editing CO: Data curation, Formal analysis, Investigation, Writing - review & editing GL: Data curation, Formal analysis, Investigation, Writing - review & editing BN: Data curation, Formal analysis, Investigation, Writing - review & editing PV: Data curation, Formal analysis, Investigation, Writing - review & editing CH: Data curation, Formal analysis, Investigation, Writing - review & editing JS: Data curation, Formal analysis, Investigation, Writing - review & editing MD: Data curation, Formal analysis, Investigation, Writing - review & editing MS: Data curation, Formal analysis, Investigation, Writing - review & editing KB: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Validation, Writing - original draft, Writing - review & editing, Visualization SD: Data curation, Formal analysis, Investigation, Writing - review & editing KP: Data curation, Formal analysis, Investigation, Writing - review & editing CS: Data curation, Formal analysis, Investigation, Writing - review & editing NM: Data curation, Formal analysis, Investigation, Writing - review & editing AP: Data curation, Formal analysis, Investigation, Writing - review & editing TW: Data curation, Formal analysis, Investigation, Writing - review & editing LK: Data curation, Formal analysis, Investigation, Writing - review & editing KE: Data curation, Formal analysis, Investigation, Writing - review & editing AM: Data curation, Formal analysis, Investigation, Writing - review & editing MH: Data curation, Formal analysis, Investigation, Writing - review & editing OD: Data curation, Formal analysis, Investigation, Project administration, Validation, Writing - review & editing, Supervision WW: Data curation, Formal analysis, Investigation, Writing - review & editing MG: Data curation, Formal analysis, Investigation, Writing - review & editing JI: Data curation, Formal analysis, Investigation, Writing - review & editing DL: Data curation, Formal analysis, Investigation, Writing - review & editing AJ: Data curation, Formal analysis, Investigation, Writing - review & editing EP: Data curation, Formal analysis, Investigation, Writing - review & editing KL: Data curation, Formal analysis, Investigation, Writing - review & editing EM: Data curation, Formal analysis, Writing - original draft, Investigation, Writing - review & editing OG: Data curation, Formal analysis, Investigation, Writing - review & editing LV: Data curation, Formal analysis, Investigation, Writing - review & editing RL: Data curation, Formal analysis, Investigation, Writing - review & editing AV: Data curation, Formal analysis, Investigation, Project administration, Validation, Funding acquisition, Writing - review & editing AQ: Data curation, Formal analysis, Investigation, Writing - review & editing SM: Data curation, Formal analysis, Investigation, Writing - review & editing JM: Data curation, Formal analysis, Investigation, Writing - review & editing MK: Data curation, Formal analysis, Investigation, Writing - review & editing AW: Data curation, Formal analysis, Investigation, Writing - review & editing EL: Data curation, Formal analysis, Investigation, Writing - review & editing JD: Data curation, Formal analysis, Investigation, Writing - review & editing LG: Data curation, Formal analysis, Investigation, Writing - review & editing OP: Data curation, Formal analysis, Investigation, Writing - review & editing MM: Data curation, Formal analysis, Investigation, Writing - review & editing ET: Data curation, Formal analysis, Investigation, Writing - review & editing, Supervision, Validation, Project administration KJ: Data curation, Formal analysis, Investigation, Writing - review & editing BC: Data curation, Formal analysis, Investigation, Writing - review & editing KW: Data curation, Formal analysis, Investigation, Writing - review & editing HS: Data curation, Formal analysis, Investigation, Supervision, Project administration, Validation, Writing - review & editing PT: Data curation, Formal analysis, Investigation, Writing - review & editing JJ: Data curation, Formal analysis, Investigation, Writing - review & editing MF: Data curation, Formal analysis, Investigation, Writing - review & editing AR: Data curation, Formal analysis, Investigation, Writing - review & editing KK: Data curation, Formal analysis, Investigation, Writing - review & editing OS: Data curation, Formal analysis, Investigation, Writing - review & editing CM: Data curation, Formal analysis, Investigation, Writing - review & editing JK: Conceptualization, Data curation, Writing - review & editing, Validation, Resources, Project administration, Funding acquisition, Methodology, Investigation, Formal analysis, Visualization KR: Data curation, Formal analysis, Investigation, Writing - review & editing JQ: Data curation, Formal analysis, Investigation, Writing - review & editing LW: Data curation, Formal analysis, Investigation, Writing - review & editing AK: Data curation, Formal analysis, Investigation, Writing - review & editing PP: Data curation, Formal analysis, Investigation, Writing - review & editing LA: Data curation, Formal analysis, Investigation, Writing - review & editing JF: Data curation, Formal analysis, Investigation, Writing - review & editing AO: Data curation, Formal analysis, Investigation, Writing - review & editing VA: Data curation, Formal analysis, Investigation, Writing - review & editing CB: Data curation, Formal analysis, Investigation, Writing - review & editing RS: Data curation, Formal analysis, Investigation, Writing - review & editing NC: Data curation, Formal analysis, Investigation, Writing - review & editing JT: Data curation, Formal analysis, Investigation, Writing - review & editing IL: Data curation, Formal analysis, Investigation, Writing - review & editing PF: Data curation, Formal analysis, Investigation, Writing - review & editing RH: Data curation, Formal analysis, Investigation, Writing - review & editing KD: Data curation, Formal analysis, Investigation, Writing - review & editing SP: Data curation, Formal analysis, Investigation, Writing - review & editing AA: Data curation, Formal analysis, Investigation, Writing - review & editing MW: Data curation, Formal analysis, Investigation, Writing - review & editing PL: Data curation, Formal analysis, Investigation, Writing - review & editing EB: Data curation, Formal analysis, Investigation, Writing - review & editing OK: Data curation, Formal analysis, Investigation, Writing - review & editing BK: Data curation, Formal analysis, Investigation, Writing - review & editing LD: Data curation, Formal analysis, Investigation, Writing - review & editing ME: Data curation, Formal analysis, Investigation, Writing - review & editing SS: Data curation, Formal analysis, Writing - review & editing, Investigation KZ: Data curation, Formal analysis, Investigation, Writing - review & editing AE: Data curation, Formal analysis, Investigation, Writing - review & editing MP: Data curation, Formal analysis, Investigation, Writing - review & editing CN: Data curation, Formal analysis, Investigation, Writing - review & editing DH: Data curation, Formal analysis, Investigation, Project administration, Validation, Writing - review & editing, Supervision DC: Data curation, Formal analysis, Investigation, Writing - review & editing VJ: Data curation, Formal analysis, Investigation, Writing - review & editing JN: Data curation, Formal analysis, Investigation, Writing - review & editing MC: Data curation, Formal analysis, Investigation, Writing - review & editing EM: Data curation, Formal analysis, Investigation, Writing - review & editing RD: Data curation, Formal analysis, Investigation, Writing - review & editing IS: Data curation, Formal analysis, Investigation, Writing - review & editing NT: Data curation, Formal analysis, Investigation, Writing - review & editing WE: Data curation, Formal analysis, Investigation, Writing - review & editing AV: Data curation, Formal analysis, Investigation, Writing - review & editing ZP: Data curation, Formal analysis, Investigation, Writing - review & editing BB: Data curation, Formal analysis, Investigation, Writing - review & editing LS: Data curation, Formal analysis, Investigation, Writing - review & editing MA: Data curation, Formal analysis, Investigation, Writing - review & editing HM: Data curation, Formal analysis, Investigation, Writing - review & editing JP: Data curation, Formal analysis, Investigation, Writing - review & editing RB: Data curation, Formal analysis, Investigation, Writing - review & editing JS: Data curation, Formal analysis, Investigation, Project administration, Supervision, Validation, Writing - review & editing |
ISSN: | 2578-9430 |
DOI: | 10.17912/micropub.biology.000542 |