Chlorella vulgaris up-modulation of myelossupression induced by lead: The role of stromal cells

In this study, Chlorella vulgaris (CV) was examined for its chelating effects on the ability of bone marrow stromal cell layer to display myeloid progenitor cells in vitro in lead-exposed mice, using the long-term bone marrow culture (LTBMC). In addition, the levels of interleukin (IL)-6, an importa...

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Published in:	Food and chemical toxicology Vol. 46; no. 9; pp. 3147 - 3154
Main Authors:	Queiroz, Mary L.S., Torello, Cristiane O., Perhs, Simone M.C., Rocha, Michelle C., Bechara, Etelvino J.H., Morgano, Marcelo A., Valadares, Marize C., Rodrigues, Ana Paula O., Ramos, Aline Lisie, Soares, Chrislaine O.
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 01-09-2008 New York, NY Elsevier Science
Subjects:	Animals Biological and medical sciences Bone marrow Bone Marrow Cells - drug effects Cells, Cultured CFU-GM Chelating Agents - chemistry Chelating Agents - pharmacology Chelation therapy Chemical and industrial products toxicology. Toxic occupational diseases Chlorella vulgaris Chlorella vulgaris - chemistry Chromatography, High Pressure Liquid Clone Cells - drug effects Hematopoietic Stem Cells - drug effects Interleukin-6 - metabolism Lead - blood Lead - toxicity Lead acetate Male Medical sciences Metals and various inorganic compounds Mice Mice, Inbred BALB C Myelopoiesis Porphyrins - biosynthesis Stem Cells - drug effects Stroma Stromal Cells - drug effects Toxicology CV IL Lead acetate LTBMC Chlorella vulgaris CSF CFU-GM Stroma Bone marrow Myelopoiesis rmGM-CSF Chelation therapy Toxicity Algae Acetate Chlorophyceae Heavy metal Treatment Chlorophyta Hematopoiesis Modulation Chelating agent Chelation Lead CFU-GM-Cell Stromal cell
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Summary:	In this study, Chlorella vulgaris (CV) was examined for its chelating effects on the ability of bone marrow stromal cell layer to display myeloid progenitor cells in vitro in lead-exposed mice, using the long-term bone marrow culture (LTBMC). In addition, the levels of interleukin (IL)-6, an important hematopoietic stimulator, as well as the numbers of adherent and non-adherent cells were also investigated. Mice were gavage treated daily with a single 50 mg/kg dose of CV for 10 days, concomitant to continuous offering of 1300 ppm lead acetate in drinking water. We found that CV up-modulates the reduced ability of stromal cell layer to display myeloid progenitor cells in vitro in lead-exposed mice and restores both the reduced number of non-adherent cells and the ability of stromal cells from these mice to produce IL-6. Monitoring of lead poisoning demonstrated that CV treatment significantly reduced lead levels in blood and tissues, completely restored the normal hepatic ALA levels, decreased the abnormally high plasma ALA and partly recovered the liver capacity to produce porphyrins. These findings provide evidence for a beneficial use of CV for combination or alternative chelating therapy to protect the host from the damage induced by lead poisoning.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0278-6915 1873-6351
DOI:	10.1016/j.fct.2008.07.005