Search Results - "Perera, Rajika L"

A structural and dynamic model for the assembly of Replication Protein A on single-stranded DNA by Yates, Luke A., Aramayo, Ricardo J., Pokhrel, Nilisha, Caldwell, Colleen C., Kaplan, Joshua A., Perera, Rajika L., Spies, Maria, Antony, Edwin, Zhang, Xiaodong

“…Replication Protein A (RPA), the major eukaryotic single stranded DNA-binding protein, binds to exposed ssDNA to protect it from nucleases, participates in a…”
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Structures of human primase reveal design of nucleotide elongation site and mode of Pol α tethering by Kilkenny, Mairi Louise, Longo, Michael Anthony, Perera, Rajika L., Pellegrini, Luca

“…Initiation of DNA synthesis in genomic duplication depends on primase, the DNA-dependent RNA polymerase that synthesizes de novo the oligonucleotides that…”
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Structural basis of quinolone inhibition of type IIA topoisomerases and target-mediated resistance by Gwynn, Michael N, Bax, Benjamin D, Wohlkonig, Alexandre, Chan, Pan F, Fosberry, Andrew P, Homes, Paul, Huang, Jianzhong, Kranz, Michael, Leydon, Vaughan R, Miles, Timothy J, Pearson, Neil D, Perera, Rajika L, Shillings, Anthony J

“…Quinolone antibacterials have been used to treat bacterial infections for over 40 years. A crystal structure of moxifloxacin in complex with Acinetobacter…”
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Beyond affinity: selection of antibody variants with optimal biophysical properties and reduced immunogenicity from mammalian display libraries by Dyson, Michael R, Masters, Edward, Pazeraitis, Deividas, Perera, Rajika L, Syrjanen, Johanna L, Surade, Sachin, Thorsteinson, Nels, Parthiban, Kothai, Jones, Philip C, Sattar, Maheen, Wozniak-Knopp, Gordana, Rueker, Florian, Leah, Rachael, McCafferty, John

“…The early phase of protein drug development has traditionally focused on target binding properties leading to a desired mode of therapeutic action. As more…”
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A comprehensive search of functional sequence space using large mammalian display libraries created by gene editing by Parthiban, Kothai, Perera, Rajika L, Sattar, Maheen, Huang, Yanchao, Mayle, Sophie, Masters, Edward, Griffiths, Daniel, Surade, Sachin, Leah, Rachael, Dyson, Michael R, McCafferty, John

“…The construction of large libraries in mammalian cells allows the direct screening of millions of molecular variants for binding properties in a cell type…”
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Mechanism for priming DNA synthesis by yeast DNA polymerase α by Perera, Rajika L, Torella, Rubben, Klinge, Sebastian, Kilkenny, Mairi L, Maman, Joseph D, Pellegrini, Luca

“…The DNA Polymerase α (Pol α)/primase complex initiates DNA synthesis in eukaryotic replication. In the complex, Pol α and primase cooperate in the production…”
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Shared active site architecture between the large subunit of eukaryotic primase and DNA photolyase by Sauguet, Ludovic, Klinge, Sebastian, Perera, Rajika L, Maman, Joseph D, Pellegrini, Luca

“…DNA synthesis during replication relies on RNA primers synthesised by the primase, a specialised DNA-dependent RNA polymerase that can initiate nucleic acid…”
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Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression by Dyson, Michael R, Shadbolt, S Paul, Vincent, Karen J, Perera, Rajika L, McCafferty, John

“…In the search for generic expression strategies for mammalian protein families several bacterial expression vectors were examined for their ability to promote…”
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A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome by Simon, Aline C., Zhou, Jin C., Perera, Rajika L., van Deursen, Frederick, Evrin, Cecile, Ivanova, Marina E., Kilkenny, Mairi L., Renault, Ludovic, Kjaer, Svend, Matak-Vinković, Dijana, Labib, Karim, Costa, Alessandro, Pellegrini, Luca

“…This study shows how the yeast Ctf4 protein couples the DNA helicase, Cdc45–MCM–GINS, to DNA polymerase α — the GINS subunit of the helicase and the polymerase…”
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A Conserved Motif in the C-terminal Tail of DNA Polymerase α Tethers Primase to the Eukaryotic Replisome by Kilkenny, Mairi L., De Piccoli, Giacomo, Perera, Rajika L., Labib, Karim, Pellegrini, Luca

“…The DNA polymerase α-primase complex forms an essential part of the eukaryotic replisome. The catalytic subunits of primase and pol α synthesize composite…”
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Flexible tethering of primase and DNA Pol α in the eukaryotic primosome by Núñez-Ramírez, Rafael, Klinge, Sebastian, Sauguet, Ludovic, Melero, Roberto, Recuero-Checa, María A., Kilkenny, Mairi, Perera, Rajika L., García-Alvarez, Begoña, Hall, Richard J., Nogales, Eva, Pellegrini, Luca, Llorca, Oscar

“…The Pol α/primase complex or primosome is the primase/polymerase complex that initiates nucleic acid synthesis during eukaryotic replication. Within the…”
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Application of phage display to high throughput antibody generation and characterization by Schofield, Darren J, Pope, Anthony R, Clementel, Veronica, Buckell, Jenny, Chapple, Susan Dj, Clarke, Kay F, Conquer, Jennie S, Crofts, Anna M, Crowther, Sandra R E, Dyson, Michael R, Flack, Gillian, Griffin, Gareth J, Hooks, Yvette, Howat, William J, Kolb-Kokocinski, Anja, Kunze, Susan, Martin, Cecile D, Maslen, Gareth L, Mitchell, Joanne N, O'Sullivan, Maureen, Perera, Rajika L, Roake, Wendy, Shadbolt, S Paul, Vincent, Karen J, Warford, Anthony, Wilson, Wendy E, Xie, Jane, Young, Joyce L, McCafferty, John

“…We have created a high quality phage display library containing over 1010 human antibodies and describe its use in the generation of antibodies on an…”
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Identification of soluble protein fragments by gene fragmentation and genetic selection by Dyson, Michael R, Perera, Rajika L, Shadbolt, S. Paul, Biderman, Lynn, Bromek, Krystyna, Murzina, Natalia V, McCafferty, John

“…We describe a new method, which identifies protein fragments for soluble expression in Escherichia coli from a randomly fragmented gene library. Inhibition of…”
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Structures of human primase reveal design of nucleotide elongation site and mode of Pol [Alpha] tethering by Kilkenny, Mairi Louise, Longo, Michael Anthony, Perera, Rajika L, Pellegrini, Luca

“…Initiation of DNA synthesis in genomic duplication depends on primase, the DNA-dependent RNA polymerase that synthesizes de novo the oligonucleotides that…”
Get full text

A Ctf4 trimer couples the CMG helicase to DNA polymerase [alpha] in the eukaryotic replisome by Simon, Aline C, Zhou, Jin C, Perera, Rajika L, van Deursen, Frederick, Evrin, Cecile, Ivanova, Marina E, Kilkenny, Mairi L, Renault, Ludovic, Kjaer, Svend, Matak-Vinkovic, Dijana, Labib, Karim, Costa, Alessandro, Pellegrini, Luca

“…Efficient duplication of the genome requires the concerted action of helicase and DNA polymerases at replication forks1 to avoid stalling of the replication…”
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Flexible tethering of primase and DNA Pol α in the eukaryotic primosome by Núñez-Ramírez, Rafael, Klinge, Sebastian, Sauguet, Ludovic, Melero, Roberto, Recuero-Checa, María A., Kilkenny, Mairi, Perera, Rajika L., García-Alvarez, Begoña, Hall, Richard J., Nogales, Eva, Pellegrini, Luca, Llorca, Oscar

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A comprehensive search of functional sequence space using large mammalian display libraries created by gene editing by Parthiban, Kothai, Perera, Rajika L., Sattar, Maheen, Huang, Yanchao, Mayle, Sophie, Masters, Edward, Griffiths, Daniel, Surade, Sachin, Leah, Rachael, Dyson, Michael R., McCafferty, John

“…The construction of large libraries in mammalian cells allows the direct screening of millions of molecular variants for binding properties in a cell type…”
Get full text

Beyond affinity: selection of antibody variants with optimal biophysical properties and reduced immunogenicity from mammalian display libraries by Dyson, Michael R., Masters, Edward, Pazeraitis, Deividas, Perera, Rajika L., Syrjanen, Johanna L., Surade, Sachin, Thorsteinson, Nels, Parthiban, Kothai, Jones, Philip C., Sattar, Maheen, Wozniak-Knopp, Gordana, Rueker, Florian, Leah, Rachael, McCafferty, John

“…The early phase of protein drug development has traditionally focused on target binding properties leading to a desired mode of therapeutic action. As more…”
Get full text