Proteomic and phosphoproteomic analyses reveal several events involved in the early stages of bovine herpesvirus 1 infection

Herpesviruses are predicted to express more than 80 proteins during their infection cycle. The proteins synthesized by the immediate early genes and early genes target signaling pathways in host cells that are essential for the successful initiation of a productive infection and for latency. In this...

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Published in:	Archives of virology Vol. 165; no. 1; pp. 69 - 85
Main Authors:	Magalhães-Junior, Marcos J., Baracat-Pereira, Maria Cristina, Pereira, Lorena K. J., Vital, Camilo E., Santos, Marcus R., Cunha, Pricila S., Fernandes, Kenner M., Bressan, Gustavo C., Fietto, Juliana L. R., Silva-Júnior, Abelardo, Almeida, Márcia R.
Format:	Journal Article
Language:	English
Published:	Vienna Springer Vienna 2020 Springer Nature B.V
Subjects:	Angiogenin Animals Biomedical and Life Sciences Biomedicine Cattle Cell Line DNA biosynthesis DNA repair Guanosine diphosphate Herpes viruses Herpesviridae Infections - metabolism Herpesviridae Infections - virology Herpesvirus 1, Bovine - metabolism Herpesvirus 1, Bovine - pathogenicity Immediate-early proteins Immediate-Early Proteins - metabolism Infections Infectious Diseases Inflammation Latency Mass Spectrometry Medical Microbiology Original Article Phosphorylation Protein Interaction Maps Proteins Proteomics - methods Ribonuclease Splicing Stathmin Stathmin - metabolism Viral Proteins - metabolism Virology Virus Replication
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Summary:	Herpesviruses are predicted to express more than 80 proteins during their infection cycle. The proteins synthesized by the immediate early genes and early genes target signaling pathways in host cells that are essential for the successful initiation of a productive infection and for latency. In this study, proteomic and phosphoproteomic tools showed the occurrence of changes in Madin-Darby bovine kidney cells at the early stage of the infection by bovine herpesvirus 1 (BoHV-1). Proteins that had already been described in the early stage of infection for other herpesviruses but not for BoHV-1 were found. For example, stathmin phosphorylation at the initial stage of infection is described for the first time. In addition, two proteins that had not been described yet in the early stages of herpesvirus infections in general were ribonuclease/angiogenin inhibitor and Rab GDP dissociation inhibitor beta. The biological processes involved in these cellular responses were repair and replication of DNA, splicing, microtubule dynamics, and inflammatory responses. These results reveal pathways that might be used as targets for designing antiviral molecules against BoHV-1 infection. Highlights BoHV-1 infection at early stages influenced various biological processes. BoHV-1 infection at early stages showed proteins reported for other virus and stages. Proteins not yet reported for BoHV-1 infection in the early stage were ribonuclease/angiogenin inhibitor and Rab GDP dissociation inhibitor beta
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0304-8608 1432-8798
DOI:	10.1007/s00705-019-04452-1