Urease activity according to the different inhibitors

Urease activity according to the different inhibitors

The objective of this study was to evaluate NBPT, zeolite and humic acid in different concentrations as inhibitors of the activity of the enzyme urease. The activity of the urease enzyme was quantified by the methods proposed by May and Douglas (1976) and Witte and Medina-Escobar (2001). For this re...

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Bibliographic Details
Published in:Anais da Academia Brasileira de Ciências Vol. 90; no. 4; pp. 3685 - 3692
Main Authors: Gomes, Mateus P, Zonta, Everaldo, Stafanato, Juliano B, Pereira, Ariana M
Format: Journal Article
Language:English
Published: Brazil Academia Brasileira de Ciências 01-10-2018
Subjects:
Enzyme Inhibitors - pharmacology
Fertilizers
humic acid
Humic Substances
MULTIDISCIPLINARY SCIENCES
nbpt
Organophosphorus Compounds - pharmacology
Soil - chemistry
urea
Urea - pharmacology
Urease - antagonists & inhibitors
zeolite
Zeolites - pharmacology
humic acid
zeolite
nbpt
urea
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Summary:The objective of this study was to evaluate NBPT, zeolite and humic acid in different concentrations as inhibitors of the activity of the enzyme urease. The activity of the urease enzyme was quantified by the methods proposed by May and Douglas (1976) and Witte and Medina-Escobar (2001). For this reason, two experiments were conducted in a completely randomized design in split plots, in which the incubation periods are the plots and the fertilizers with the inhibitors at the different concentrations are the subplots with three replications. Fifteen fertilizers based on NBPT-coated urea, humic acid and zeolite were used in different concentrations. The two methods of analysis used in the experiment confirmed the efficiency of NBPT in inhibiting the enzymatic action even at low concentration, with 0.02% (m/m) being sufficient to inhibit urease, with no difference between inhibitor concentrations. The addition of humic acid reduced the activity of urease after 36 hours of reaction when the activity of the enzyme was evaluated by the method proposed by May and Douglas (1976). Zeolite did not influence the activity of the urease enzyme when analyzed by the May and Douglas method (1976).
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ISSN:0001-3765
1678-2690
1678-2690
DOI:10.1590/0001-3765201820170636