Ultrabright Silica-Coated Organic Nanocrystals for Two-Photon In Vivo Imaging

Ultrabright Silica-Coated Organic Nanocrystals for Two-Photon In Vivo Imaging

Herein, we demonstrate the potential of ultrabright fluorescent silica-coated organic nanocrystals for two-photon in vivo imaging. These unique nanoparticles (NPs) containing a crystalline core of small push–pull dipolar dye are specifically designed to exhibit two-photon absorption for fluorescence...

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Bibliographic Details
Published in:ACS applied nano materials Vol. 3; no. 12; pp. 11933 - 11944
Main Authors: Perdoor, Shridevi Shenoi, Dubois, Fabien, Barbara, Aude, Nguyen, Christophe, Ali, Lamiaa M. A, Gary-Bobo, Magali, Hristovska, Ines, Combet, Kassandre, Pascual, Olivier, Micouin, Guillaume, Banyasz, Akos, Bretonnière, Yann, Andraud, Chantal, Ibanez, Alain, Cattoën, Xavier
Format: Journal Article
Language:English
Published: American Chemical Society 24-12-2020
Subjects:
Chemical Sciences
Material chemistry
Organic chemistry
two-photon absorption
dynamic light scattering
nanoparticles
colloidal stabilization
bioimaging
Nanoparticles
Colloidal stabilization
Two-photon absorption
Dynamic light scattering
Bio-imaging
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Summary:Herein, we demonstrate the potential of ultrabright fluorescent silica-coated organic nanocrystals for two-photon in vivo imaging. These unique nanoparticles (NPs) containing a crystalline core of small push–pull dipolar dye are specifically designed to exhibit two-photon absorption for fluorescence imaging. The NPs can be easily functionalized using click chemistry in pure water, with preservation of the organic core. A novel small-volume DLS technique was used to evaluate the effect of PEGylation on the colloidal stability of the NPs in complex media containing salts and proteins, mimicking the composition of blood serum. The potential of these bright red-emitting NPs for two-photon fluorescence imaging is demonstrated both in vitro and in vivo.
ISSN:2574-0970
2574-0970
DOI:10.1021/acsanm.0c02499