Search Results - "Perchetti, Garrett A."

Comparative Performance of SARS-CoV-2 Detection Assays Using Seven Different Primer-Probe Sets and One Assay Kit by Nalla, Arun K, Casto, Amanda M, Huang, Meei-Li W, Perchetti, Garrett A, Sampoleo, Reigran, Shrestha, Lasata, Wei, Yulun, Zhu, Haiying, Jerome, Keith R, Greninger, Alexander L

“…Nearly 400,000 people worldwide are known to have been infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) beginning in December 2019…”
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Validation of SARS-CoV-2 detection across multiple specimen types by Perchetti, Garrett A., Nalla, Arun K., Huang, Meei-Li, Zhu, Haiying, Wei, Yulun, Stensland, Larry, Loprieno, Michelle A., Jerome, Keith R., Greninger, Alexander L.

“…•Modified CDC-based assays can detect SARS-CoV-2 across a range of sample types.•SARS-CoV-2 N1 assay detection sensitivity was comparable between specimen…”
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Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step by Bruce, Emily A, Huang, Meei-Li, Perchetti, Garrett A, Tighe, Scott, Laaguiby, Pheobe, Hoffman, Jessica J, Gerrard, Diana L, Nalla, Arun K, Wei, Yulun, Greninger, Alexander L, Diehl, Sean A, Shirley, David J, Leonard, Debra G B, Huston, Christopher D, Kirkpatrick, Beth D, Dragon, Julie A, Crothers, Jessica W, Jerome, Keith R, Botten, Jason W

“…The ongoing COVID-19 pandemic has created an unprecedented need for rapid diagnostic testing. The World Health Organization (WHO) recommends a standard assay…”
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Multiplexing primer/probe sets for detection of SARS-CoV-2 by qRT-PCR by Perchetti, Garrett A., Nalla, Arun K., Huang, Meei-Li, Jerome, Keith R., Greninger, Alexander L.

“…•Multiplexing three targets in a single-reaction maintains LDT assay performance.•Triplexing correctly identified SARS-CoV-2 in 98.4% positive or inconclusive…”
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Author Correction: Positive selection underlies repeated knockout of ORF8 in SARS-CoV-2 evolution by Wagner, Cassia, Kistler, Kathryn E., Perchetti, Garrett A., Baker, Noah, Frisbie, Lauren A., Torres, Laura Marcela, Aragona, Frank, Yun, Cory, Figgins, Marlin, Greninger, Alexander L., Cox, Alex, Oltean, Hanna N., Roychoudhury, Pavitra, Bedford, Trevor

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Positive selection underlies repeated knockout of ORF8 in SARS-CoV-2 evolution by Wagner, Cassia, Kistler, Kathryn E., Perchetti, Garrett A., Baker, Noah, Frisbie, Lauren A., Torres, Laura Marcela, Aragona, Frank, Yun, Cory, Figgins, Marlin, Greninger, Alexander L., Cox, Alex, Oltean, Hanna N., Roychoudhury, Pavitra, Bedford, Trevor

“…Knockout of the ORF8 protein has repeatedly spread through the global viral population during SARS-CoV-2 evolution. Here we use both regional and global…”
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Predicting infectivity: comparing four PCR‐based assays to detect culturable SARS‐CoV‐2 in clinical samples by Bruce, Emily A, Mills, Margaret G, Sampoleo, Reigran, Perchetti, Garrett A, Huang, Meei‐Li, Despres, Hannah W, Schmidt, Madaline M, Roychoudhury, Pavitra, Shirley, David J, Jerome, Keith R, Greninger, Alexander L, Botten, Jason W

“…With the COVID‐19 pandemic caused by SARS‐CoV‐2 now in its second year, there remains an urgent need for diagnostic testing that can identify infected…”
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Detection of SARS-CoV-2 by bronchoscopy after negative nasopharyngeal testing: Stay vigilant for COVID-19 by Ramos, Kathleen J., Kapnadak, Siddhartha G., Collins, Bridget F., Pottinger, Paul S., Wall, Richard, Mays, James A., Perchetti, Garrett A., Jerome, Keith R., Khot, Sandeep, Limaye, Ajit P., Mathias, Patrick C., Greninger, Alexander

“…Real-time polymerase chain reaction (RT-PCR) detection of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is required for diagnosis of coronavirus…”
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Analytical Sensitivity of the Abbott BinaxNOW COVID-19 Ag Card by Perchetti, Garrett A, Huang, Meei-Li, Mills, Margaret G, Jerome, Keith R, Greninger, Alexander L

“…Multiple rapid antigen (Ag) tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have recently received emergency-use authorization (EUA)…”
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Stability of SARS-CoV-2 in Phosphate-Buffered Saline for Molecular Detection by Perchetti, Garrett A, Huang, Meei-Li, Peddu, Vikas, Jerome, Keith R, Greninger, Alexander L

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Pooling of SARS-CoV-2 samples to increase molecular testing throughput by Perchetti, Garrett A., Sullivan, Ka-Wing, Pepper, Greg, Huang, Meei-Li, Breit, Nathan, Mathias, Patrick, Jerome, Keith R., Greninger, Alexander L.

“…•Four-way pooling correctly identified SARS-CoV-2 in 94 % of positive samples (n = 30/32) tested.•Low viral loads, corresponding with late CTs, may be missed…”
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Metagenomic Analysis Reveals Clinical SARS-CoV-2 Infection and Bacterial or Viral Superinfection and Colonization by Peddu, Vikas, Shean, Ryan C, Xie, Hong, Shrestha, Lasata, Perchetti, Garrett A, Minot, Samuel S, Roychoudhury, Pavitra, Huang, Meei-Li, Nalla, Arun, Reddy, Shriya B, Phung, Quynh, Reinhardt, Adam, Jerome, Keith R, Greninger, Alexander L

“…Abstract Background More than 2 months separated the initial description of SARS-CoV-2 and discovery of its widespread dissemination in the United States…”
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A SARS-CoV-2 Nucleocapsid Variant that Affects Antigen Test Performance by Bourassa, Lori, Perchetti, Garrett A., Phung, Quynh, Lin, Michelle J., Mills, Margaret G., Roychoudhury, Pavitra, Harmon, Kimberly G., Reed, Jonathan C., Greninger, Alexander L.

“…•The quidel sofia SARS antigen FIA test (Sofia 2) has an analytical sensitivity of ~54,000 copies/mL.•The sofia 2 had a 1000-fold lower sensitivity for…”
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Cytomegalovirus breakthrough and resistance during letermovir prophylaxis by Perchetti, Garrett A., Biernacki, Melinda A., Xie, Hu, Castor, Jared, Joncas-Schronce, Laurel, Ueda Oshima, Masumi, Kim, YoungJun, Jerome, Keith R., Sandmaier, Brenda M., Martin, Paul J., Boeckh, Michael, Greninger, Alexander L., Zamora, Danniel

“…Letermovir is a relatively new antiviral for prophylaxis against cytomegalovirus (CMV) after allogeneic hematopoietic cell transplantation (HCT)…”
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Specific allelic discrimination of N501Y and other SARS‐CoV‐2 mutations by ddPCR detects B.1.1.7 lineage in Washington State by Perchetti, Garrett A., Zhu, Haiying, Mills, Margaret G., Shrestha, Lasata, Wagner, Cassia, Bakhash, Shah Mohamed, Lin, Michelle J., Xie, Hong, Huang, Meei‐Li, Mathias, Patrick, Bedford, Trevor, Jerome, Keith R., Greninger, Alexander L., Roychoudhury, Pavitra

“…Real‐time epidemiological tracking of variants of concern (VOCs) can help limit the spread of more contagious forms of severe acute respiratory syndrome…”
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Performance characteristics of the Abbott Alinity m SARS-CoV-2 assay by Perchetti, Garrett A., Pepper, Gregory, Shrestha, Lasata, LaTurner, Katrina, Yae Kim, Da, Huang, Meei-Li, Jerome, Keith R., Greninger, Alexander L.

“…•Abbott Alinity m is a new high-throughput, sample-to-answer platform for qRT-PCR.•We compared the Alinity m SARS-CoV-2 to the Roche cobas and Hologic Panther…”
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Rapid and accurate identification of SARS-CoV-2 Omicron variants using droplet digital PCR (RT-ddPCR) by Mills, Margaret G., Hajian, Pooneh, Bakhash, Shah Mohamed, Xie, Hong, Mantzke, Derrek, Zhu, Haiying, Perchetti, Garrett A., Huang, Meei-Li, Pepper, Gregory, Jerome, Keith R., Roychoudhury, Pavitra, Greninger, Alexander L.

“…•Some mutations in SARS-CoV-2 Spike correlate with immune evasion in many variants.•RT-ddPCR can quickly and accurately identify these causative single base…”
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Large, Stable, Contemporary Interspecies Recombination Events in Circulating Human Herpes Simplex Viruses by Casto, Amanda M, Roychoudhury, Pavitra, Xie, Hong, Selke, Stacy, Perchetti, Garrett A, Wofford, Haley, Huang, Meei-Li, Verjans, Georges M G M, Gottlieb, Geoffrey S, Wald, Anna, Jerome, Keith R, Koelle, David M, Johnston, Christine, Greninger, Alexander L

“…Abstract Background The ubiquitous human pathogens, herpes simplex virus (HSV)-1 and HSV-2, are distinct viral species that diverged approximately 6 million…”
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Phylogenetic characterization of rhinoviruses from infants in Sarlahi, Nepal by Kuypers, Jane, Perchetti, Garrett A., Chu, Helen Y., Newman, Kira L., Katz, Joanne, Khatry, Subarna K., LeClerq, Steven C., Jerome, Keith R., Tielsch, James M., Englund, Janet A.

“…Problem Rhinoviruses (RVs), the most common causes of acute respiratory infections in young children and infants, are highly diverse genetically. Objective To…”
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Human Metapneumovirus Infection and Genotyping of Infants in Rural Nepal by Perchetti, Garrett A, Wilcox, Naomi, Chu, Helen Y, Katz, Joanne, Khatry, Subarna K, LeClerq, Steven C, Tielsch, James M, Jerome, Keith R, Englund, Janet A, Kuypers, Jane

“…Acute respiratory tract infections are a serious clinical burden in infants; human metapneumovirus (HMPV) is an important etiological agent. We investigated…”
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