Genomic and transcriptomic analyses of thyroid cancers identify DICER1 somatic mutations in adult follicular-patterned RAS-like tumors

Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer (TC). Several genomic and transcriptomic studies explored the molecular landscape of follicular cell-derived TCs, and V600E, mutations, and gene fusions are well-established drivers. mutations were described in specific sets...

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Published in:	Frontiers in endocrinology (Lausanne) Vol. 14; p. 1267499
Main Authors:	Minna, Emanuela, Devecchi, Andrea, Pistore, Federico, Paolini, Biagio, Mauro, Giuseppe, Penso, Donata Alda, Pagliardini, Sonia, Busico, Adele, Pruneri, Giancarlo, De Cecco, Loris, Borrello, Maria Grazia, Sensi, Marialuisa, Greco, Angela
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Media S.A 05-10-2023
Subjects:	Adult DEAD-box RNA Helicases - genetics DICER1 Endocrinology Genomics Humans Mutation mutations Protein-Tyrosine Kinases Proto-Oncogene Proteins - genetics Retrospective Studies Ribonuclease III - genetics thyroid cancer Thyroid Cancer, Papillary - genetics Thyroid Cancer, Papillary - pathology Thyroid Neoplasms - genetics Thyroid Neoplasms - pathology Transcriptome transcriptomics whole exome sequencing transcriptomics thyroid cancer mutations DICER1 whole exome sequencing
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Summary:	Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer (TC). Several genomic and transcriptomic studies explored the molecular landscape of follicular cell-derived TCs, and V600E, mutations, and gene fusions are well-established drivers. mutations were described in specific sets of TC patients but represent a rare event in adult TC patients. Here, we report the molecular characterization of 30 retrospective follicular cell-derived thyroid tumors, comprising PTCs (90%) and poorly differentiated TCs (10%), collected at our Institute. We performed DNA whole-exome sequencing using patient-matched control for somatic mutation calling, and targeted RNA-seq for gene fusion detection. Transcriptional profiles established in the same cohort by microarray were investigated using three signaling-related gene signatures derived from The Cancer Genome Atlas (TCGA). The occurrence of V600E (44%), mutations (13%), and gene fusions (13%) was confirmed in our cohort. In addition, in two patients lacking known drivers, mutations of the gene (p.D1709N and p.D1810V) were identified. mutations occur in two adult patients with follicular-pattern lesions, and in one of them a second concurrent mutation (p.R459*) is also observed. Additional putative drivers include gene (p.P2130A mutation), identified in a patient with a rare solid-trabecular subtype of PTC. Transcriptomics indicates that tumors are RAS-like, whereas the -mutated tumor displays a borderline RAS-/BRAF-like subtype. We also provide an overview of and mutations in thyroid lesions by investigating the COSMIC database. Even though small, our series recapitulates the genetic background of PTC. Furthermore, we identified mutations, one of which is previously unreported in thyroid lesions. For these less common alterations and for patients with unknown drivers, we provide signaling information applying TCGA-derived classification.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Julio Ricarte-Filho, Children’s Hospital of Philadelphia, United States; Frederique Savagner, INSERM U1048 Institut des Maladies Métaboliques et Cardiovasculaires, France These authors share last authorship Present address: Giuseppe Mauro, Eurofins Biolab, Milan, Italy Edited by: Juan Pablo Nicola, National University of Cordoba, Argentina
ISSN:	1664-2392 1664-2392
DOI:	10.3389/fendo.2023.1267499