In situ localization of mRNAs coding for mouse testicular structural genes

In situ localization of mRNAs coding for mouse testicular structural genes

In situ hybridization histochemistry has been used to localize mRNA transcripts of five nuclear and cytoplasmic structural genes in the mouse testis. The mRNAs for three nuclear structural proteins involved in chromatin transformation during spermatogenesis (the two protamine variants of the mouse a...

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Bibliographic Details
Published in:Experimental cell research Vol. 173; no. 1; p. 274
Main Authors: Hecht, N B, Penshow, J D
Format: Journal Article
Language:English
Published: United States 01-11-1987
Subjects:
Actins - genetics
Animals
Cell Compartmentation
DNA-Binding Proteins - genetics
Intracellular Signaling Peptides and Proteins
Male
Mice
Microtubule-Associated Proteins
Nuclear Proteins - genetics
Nucleic Acid Hybridization
Protamines - genetics
RNA, Messenger - metabolism
Spermatogenesis
t-Complex Genome Region
Testis - metabolism
Testis - ultrastructure
Tubulin - genetics
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Summary:In situ hybridization histochemistry has been used to localize mRNA transcripts of five nuclear and cytoplasmic structural genes in the mouse testis. The mRNAs for three nuclear structural proteins involved in chromatin transformation during spermatogenesis (the two protamine variants of the mouse and one of the testis-specific proteins) are restricted solely to postmeiotic germ cells. In contrast, mRNAs for two other structural proteins, actin and alpha tubulin, are detected throughout spermatogenesis. Although present in premeiotic, meiotic, and postmeiotic cell types, the mRNA levels of actin and alpha tubulin differ considerably during spermiogenesis, the haploid phase of spermatogenesis. Actin mRNA levels decrease markedly as the male gamete differentiates during spermiogenesis whereas alpha-tubulin mRNAs are equally abundant in the haploid round and elongating spermatids.
ISSN:0014-4827
DOI:10.1016/0014-4827(87)90353-3