Visualization and Quantification of the Oral Hygiene Effects of Brushing, Dentifrice Use, and Brush Wear Using a Tooth Brushing Simulator

Approaches that reproduce dental hygiene regimens under controlled conditions have applications in preclinical research. We have applied standardized, reproducible brushing regimes to typodonts coated in simulated or biological plaques to assess the effects on tooth cleaning of toothbrush/dentifrice...

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Bibliographic Details
Published in:	Frontiers in public health Vol. 7; p. 91
Main Authors:	Ledder, Ruth G, Latimer, Joe, Forbes, Sarah, Penney, Jodie L, Sreenivasan, Prem K, McBain, Andrew J
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Media S.A 08-05-2019
Subjects:	biofilm model simulated plaque brush wear brushing simulator dental plaque Public Health typodont typodont dental plaque brushing simulator brush wear biofilm model simulated plaque
Online Access:	Get full text
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Summary:	Approaches that reproduce dental hygiene regimens under controlled conditions have applications in preclinical research. We have applied standardized, reproducible brushing regimes to typodonts coated in simulated or biological plaques to assess the effects on tooth cleaning of toothbrush/dentifrice regimens. Replicated typodonts were coated with Occlude or Glogerm indicators to simulate plaque, and brushed reproducibly using a mechanical brushing simulator to compare the cleaning of occlusal surfaces before and after brushing with water or a dentifrice. An model using salivary inocula to cultivate oral biofilms on typodont surfaces was then developed to evaluate removal of disclosed plaque by new toothbrushes in comparison to toothbrushes with wear equivalent to 3 months of use. Analyses of typodonts brushed under controlled conditions significantly ( < 0.01) distinguished between brushed and unbrushed surfaces and between the use of water vs. dentifrice for the removal of simulated interproximal plaque ( < 0.05). New toothbrushes removed significantly ( < 0.05) more biological plaque from typodont surfaces than brushes that had been worn by repeated brushing. Through controlled and defined brushing of typodonts with simulated and biological plaques, the effectiveness of dental hygiene regimens was compared under reproducible conditions. Data indicate that the cleaning effectiveness of brushing was augmented by the addition of dentifrice and that new brushes were significantly more effective than brushes with substantial wear from previous use. Whilst we have focussed on the occlusal surfaces of molars and worn brushes, the method could be applied to a range of other tooth surfaces and oral hygiene regimens.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Tamanna Tiwari, University of Colorado Denver, United States Present Address: Joe Latimer, School of Environment and Life Sciences, University of Salford, Salford, United Kingdom Reviewed by: Florence Carrouel, Université Claude Bernard Lyon 1, France; Patricia Silveira Martins, Independent Researcher, Brazil Sarah Forbes, Department of Biosciences and Chemistry, Sheffield Hallam University, Sheffield, United Kingdom This article was submitted to Public Health Education and Promotion, a section of the journal Frontiers in Public Health Joint first authors
ISSN:	2296-2565 2296-2565
DOI:	10.3389/fpubh.2019.00091