Superior field performance of waxy corn engineered using CRISPR–Cas9

We created waxy corn hybrids by CRISPR–Cas9 editing of a waxy allele in 12 elite inbred maize lines, a process that was more than a year faster than conventional trait introgression using backcrossing and marker-assisted selection. Field trials at 25 locations showed that CRISPR-waxy hybrids were ag...

Full description

Saved in:

Bibliographic Details
Published in:	Nature biotechnology Vol. 38; no. 5; pp. 579 - 581
Main Authors:	Gao, Huirong, Gadlage, Mark J., Lafitte, H. Renee, Lenderts, Brian, Yang, Meizhu, Schroder, Megan, Farrell, Jeffry, Snopek, Kay, Peterson, Dave, Feigenbutz, Lanie, Jones, Spencer, St Clair, Grace, Rahe, Melissa, Sanyour-Doyel, Nathalie, Peng, Chenna, Wang, Lijuan, Young, Joshua K., Beatty, Mary, Dahlke, Brian, Hazebroek, Jan, Greene, Thomas W., Cigan, A. Mark, Chilcoat, N. Doane, Meeley, R. Bob
Format:	Journal Article
Language:	English
Published:	New York Nature Publishing Group US 01-05-2020 Nature Publishing Group
Subjects:	631/1647 631/337 631/449 631/61 Agriculture Agronomy Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Brief Communication Corn CRISPR CRISPR-Cas Systems Crop Production Crop yields Gene Editing - methods Genetic Introgression Genetic modification Hybrids Inbreeding Life Sciences Marker-assisted selection Neomycin Plant Proteins - genetics Quantitative Trait Loci Sequence Deletion Zea mays - genetics Zea mays - growth & development
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	We created waxy corn hybrids by CRISPR–Cas9 editing of a waxy allele in 12 elite inbred maize lines, a process that was more than a year faster than conventional trait introgression using backcrossing and marker-assisted selection. Field trials at 25 locations showed that CRISPR-waxy hybrids were agronomically superior to introgressed hybrids, producing on average 5.5 bushels per acre higher yield. Gene-edited waxy corn lines have higher yields in field trials than hybrids produced by traditional trait introgression.
ISSN:	1087-0156 1546-1696
DOI:	10.1038/s41587-020-0444-0