The nucleotide sequence of satellite tobacco necrosis virus strain C and helper-assisted replication of wild-type and mutant clones of the virus

DH Bringloe, AP Gultyaev, M Pelpel, CW Pleij and RH Coutts Biology Department, Imperial College of Science, Technology and Medicine, London, UK. The complete nucleotide sequence of satellite tobacco necrosis virus strain C (STNV-C) was determined. The genome has a similar overall organization to two...

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Bibliographic Details
Published in:	Journal of general virology Vol. 79; no. 6; pp. 1539 - 1546
Main Authors:	Bringloe, DH, Gultyaev, AP, Pelpel, M, Pleij, CW, Coutts, RH
Format:	Journal Article
Language:	English
Published:	England Soc General Microbiol 01-06-1998
Subjects:	Base Sequence DNA, Viral Helper Viruses - genetics Helper Viruses - physiology Molecular Sequence Data Mutation Nicotiana - virology Nucleic Acid Conformation Plants, Toxic RNA, Viral - chemistry Satellite Viruses - genetics Satellite Viruses - physiology Sequence Analysis, RNA Virus Replication
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Summary:	DH Bringloe, AP Gultyaev, M Pelpel, CW Pleij and RH Coutts Biology Department, Imperial College of Science, Technology and Medicine, London, UK. The complete nucleotide sequence of satellite tobacco necrosis virus strain C (STNV-C) was determined. The genome has a similar overall organization to two STNV isolates studied previously but differs significantly from them in the secondary structure of the translated and untranslated regions (UTRs). STNV-C RNA is naturally uncapped and contains 1221 nt: 101 nt in the 5' UTR, 606 nt in the capsid protein (CP) coding region and 514 nt in the 3' UTR. Using the known sequences of STNV-C and tobacco necrosis virus strain D (TNV-D) RNAs, full-length cDNA clones of both RNAs were constructed. Synthetic transcripts derived from STNV-C cDNA clones only replicated in plants and protoplasts when co-inoculated with TNV-D transcripts. A number of mutant clones in both the 3' and the 5' STNV-C RNA UTRs were constructed which disrupted putative cis-acting elements recognized by helper virus polymerase. Deletion analysis revealed an essential requirement of all 3' and 5' proximal sequences in the STNV-C UTRs for replication. However, an internal region in the 3' UTR could be deleted without loss of infectivity. Likewise, the entire STNV-C CP-encoding region could be deleted and replaced with a marker gene of a similar size without loss of transcript accumulation in plants.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0022-1317 1465-2099
DOI:	10.1099/0022-1317-79-6-1539