Search Results - "Pellegrini, Vanessa O.A."
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Combining pieces: a thorough analysis of light activation boosting power and co-substrate preferences for the catalytic efficiency of lytic polysaccharide monooxygenase MtLPMO9A
Published in Biofuel research journal (01-09-2021)“…Cost-efficient plant biomass conversion using biochemical and/or chemical routes is essential for transitioning to sustainable chemical technologies and…”
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Impact of cellulose properties on enzymatic degradation by bacterial GH48 enzymes: Structural and mechanistic insights from processive Bacillus licheniformis Cel48B cellulase
Published in Carbohydrate polymers (15-07-2021)“…[Display omitted] •Structural mechanism of cellulose depolymerization by GH48 cellulase was examined.•BlCel48B has high processivity against crystalline and…”
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Cellulose fiber size defines efficiency of enzymatic hydrolysis and impacts degree of synergy between endo- and exoglucanases
Published in Cellulose (London) (01-03-2018)“…An interplay between cellulases is fundamental in biomass saccharification. Here, the synergistic action of Trichoderma harzianum Cel7A and Cel7B on two…”
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Recombinant Trichoderma harzianum endoglucanase I (Cel7B) is a highly acidic and promiscuous carbohydrate-active enzyme
Published in Applied microbiology and biotechnology (01-11-2015)“…Trichoderma filamentous fungi have been investigated due to their ability to secrete cellulases which find various biotechnological applications such as…”
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Reversibility of Substrate Adsorption for the Cellulases Cel7A, Cel6A, and Cel7B from Hypocrea jecorina
Published in Langmuir (28-10-2014)“…Adsorption of cellulases on the cellulose surface is an integral part of the catalytic mechanism, and a detailed description of the adsorption process is…”
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Structural and molecular dynamics investigations of ligand stabilization via secondary binding site interactions in Paenibacillus xylanivorans GH11 xylanase
Published in Computational and structural biotechnology journal (01-01-2021)“…[Display omitted] •PxXyn11B is the first crystal structure of a GH11 from Paenibacillus.•X-ray structure of PxXyn11B reveals its molecular architecture and a…”
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An overview on progress, advances, and future outlook for biohydrogen production technology
Published in International journal of hydrogen energy (30-10-2022)“…The hydrogen (H2) economy has been recognized globally from a socio-economic, and environmental viewpoint. Hydrogen can be produced using technologies such as…”
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Production of prebiotic xylooligosaccharides from arabino- and glucuronoxylan using a two-domain Jonesia denitrificans xylanase from GH10 family
Published in Enzyme and microbial technology (01-03-2021)“…•JdXyn10A is a two-domain GH10 xylanase.•JdXyn10A is halotolerant and ethanol resistant.•A tightening of the +2 subsite prevent the enzyme from releasing…”
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Cellulose nanofibers production using a set of recombinant enzymes
Published in Carbohydrate polymers (15-03-2021)“…•Cellulose nanofibers (CNF) were produced combining hydrolytic and oxidative enzymes.•The set of recombinant enzymes was applied to sugarcane bagasse for the…”
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Transformation of xylan into value-added biocommodities using Thermobacillus composti GH10 xylanase
Published in Carbohydrate polymers (01-11-2020)“…•T. composti GH10 xylanase is thermostable, halotolerant and ethanol-resistant.•X-ray structure of TcXyn10A reveals molecular details of xylan…”
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Enhanced hydrolysis of hydrothermally and autohydrolytically treated sugarcane bagasse and understanding the structural changes leading to improved saccharification
Published in Biomass & bioenergy (01-08-2020)“…The enzymatic mixture containing Novozyme Cellic CTec2 spiked with xylanase derived from Thermomyces lanuginosus (1000 U xylanase per g of substrate) resulted…”
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Liquid ammonia pretreatment optimization for improved release of fermentable sugars from sugarcane bagasse
Published in Journal of cleaner production (25-01-2021)“…Alkali pretreatment of biomass is a widely accepted method for efficient delignification causing significant recovery of pentoses and hexoses to be utilized…”
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Physical techniques shed light on the differences in sugarcane bagasse structure subjected to steam explosion pretreatments at equivalent combined severity factors
Published in Industrial crops and products (15-12-2020)“…•Samples pretreated at equivalent CSFs showed significant physical and chemical differences.•CSF is not robust enough to fully predict the effects of the…”
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Light Boosts the Activity of Novel LPMO from Aspergillus fumigatus Leading to Oxidative Cleavage of Cellulose and Hemicellulose
Published in ACS sustainable chemistry & engineering (19-12-2022)“…Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that potentiate the deconstruction of polysaccharides through an oxidative mechanism…”
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Unlocking the structural features for the xylobiohydrolase activity of an unusual GH11 member identified in a compost‐derived consortium
Published in Biotechnology and bioengineering (01-10-2021)“…The heteropolysaccharide xylan is a valuable source of sustainable chemicals and materials from renewable biomass sources. A complete hydrolysis of this major…”
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Light-stimulated T. thermophilus two-domain LPMO9H: Low-resolution SAXS model and synergy with cellulases
Published in Carbohydrate polymers (15-05-2021)“…[Display omitted] •TtLPMO9H oxidizes cellulose at C1 and C4 positions.•Combined activity of TtLPMO9H and GH7 endoglucanase remarkably increased yeild of…”
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Optimization of Dilute Acid Pretreatment for Enhanced Release of Fermentable Sugars from Sugarcane Bagasse and Validation by Biophysical Characterization
Published in Bioenergy research (01-03-2023)“…Pretreatment of biomass is one of the most challenging steps in the process of second-generation (2G) ethanol and biochemical production. Dilute acid…”
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Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides
Published in Biochimica et biophysica acta. General subjects (01-06-2019)“…Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for…”
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Molecular characterization of a family 5 glycoside hydrolase suggests an induced-fit enzymatic mechanism
Published in Scientific reports (01-04-2016)“…Glycoside hydrolases (GHs) play fundamental roles in the decomposition of lignocellulosic biomaterials. Here, we report the full-length structure of a…”
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Leaves from four different sugarcane varieties as potential renewable feedstocks for second-generation ethanol production: Pretreatments, chemical composition, physical structure, and enzymatic hydrolysis yields
Published in Biocatalysis and agricultural biotechnology (01-10-2022)“…Development of Bioeconomy is impossible without establishing efficient technologies of lignocellulosic biomass valorization via transformation into fermentable…”
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