Retrieval and purification of human β cells from stem cell-derived islet engrafted mice

Stem cell-derived beta cells (SC-β-cells) engrafted into mice serve as a pre-clinical model of diabetes. It is helpful to recover viable β cells following transplantation to perform tests on the graft. We developed a protocol to retrieve and purify a sufficient number of live β cells from mice follo...

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Bibliographic Details
Published in:	STAR protocols Vol. 2; no. 3; p. 100675
Main Authors:	Kenty, Jennifer Hyoje-Ryu, Pelayo, Gabriela Cristina, Leite, Nayara Carvalho
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 17-09-2021 Elsevier
Subjects:	Cell Biology Cell isolation Organoids Protocol Stem Cells Stem Cells Organoids Cell isolation Cell Biology
Online Access:	Get full text
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Summary:	Stem cell-derived beta cells (SC-β-cells) engrafted into mice serve as a pre-clinical model of diabetes. It is helpful to recover viable β cells following transplantation to perform tests on the graft. We developed a protocol to retrieve and purify a sufficient number of live β cells from mice following long-term human SC-β-cell engraftment. The protocol enables examination of SC-β-cells undergoing developmental and metabolic changes in vivo and may facilitate the understanding of metabolic demand on SC-β-cells. [Display omitted] •Retrieval of a large number of highly viable stem cell-derived β cells (SC-β-cells)•Purification of graft cells using target cell-specific magnetic sorting•Molecular and functional graft cell analysis Stem cell-derived beta cells (SC-β-cells) engrafted into mice serve as a pre-clinical model of diabetes. It is helpful to recover viable β cells following transplantation to perform tests on the graft. We developed a protocol to retrieve and purify a sufficient number of live β cells from mice following long-term human SC-β-cell engraftment. The protocol enables examination of SC-β-cells undergoing developmental and metabolic changes in vivo and may facilitate the understanding of metabolic demand on SC-β-cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Technical contact Lead contact
ISSN:	2666-1667 2666-1667
DOI:	10.1016/j.xpro.2021.100675