Search Results - "Pekar, H."

Evaluation of indicators for cyanobacterial risk in 108 temperate lakes using 23 years of environmental monitoring data by Li, J., Persson, K. M., Pekar, H., Jansson, D.

“…Background Cyanobacterial blooms are of increasing concern for drinking water supply. In Sweden, a survey among drinking water producers showed that the sense…”
Get full text

Quantitation of aggregate levels in a recombinant humanized monoclonal antibody formulation by size-exclusion chromatography, asymmetrical flow field flow fractionation, and sedimentation velocity by Gabrielson, John P, Brader, Mark L, Pekar, Allen H, Mathis, Kathrin B, Winter, Gerhard, Carpenter, John F, Randolph, Theodore W

“…Size-exclusion high-performance liquid chromatography (SE-HPLC, SEC) is the long-standing biopharmaceutical industry standard for quantitation of soluble…”
Get more information

Opalescent appearance of an IgG1 antibody at high concentrations and its relationship to noncovalent association by Sukumar, Muppalla, Doyle, Brandon L, Combs, Jessica L, Pekar, Allen H

“…Therapeutic antibodies are often formulated at a high concentration where they may have an opalescent appearance. The aim of this study is to understand the…”
Get full text

Hybrid insulin cocrystals for controlled release delivery by Brader, Mark L, Myers, Sharon R, Sukumar, Muppalla, Pekar, Allen H, McClellan, David S, Chance, Ronald E, Flora, David B, Cox, Amy L, Irwin, Lynnie

“…The ability to tailor the release profile of a drug by manipulating its formulation matrix offers important therapeutic advantages. We show here that human…”
Get full text

Altering the association properties of insulin by amino acid replacement by Brems, D N, Alter, L A, Beckage, M J, Chance, R E, DiMarchi, R D, Green, L K, Long, H B, Pekar, A H, Shields, J E, Frank, B H

“…The importance of ProB28 and LysB29 on the self-association of insulin was established by systematically truncating the C terminus of the B chain. The…”
Get more information

Effects of non-covalent self-association on the subcutaneous absorption of a therapeutic peptide by CLODFELTER, D. K, PEKAR, A. H, REBHUN, D. M, DESTRAMPE, K. A, HAVEL, H. A, MYERS, S. R, BRADER, M. L

“…To utilize an acylated peptide as a model system to investigate the relationships among solution peptide conformation, non-covalent self-association,…”
Get full text

Biophysical signatures of noncovalent aggregates formed by a glucagonlike peptide-1 analog: a prototypical example of biopharmaceutical aggregation by Doyle, Brandon L, Pollo, Mark J, Pekar, Allen H, Roy, Michael L, Thomas, Beth Ann, Brader, Mark L

“…LY307161 is a 31 amino acid analog of glucagonlike peptide-1(7-37)OH susceptible to physical instability associated with pharmaceutical processing. Orthogonal…”
Get more information

Evidence for a self-associating equilibrium intermediate during folding of human growth hormone by DeFelippis, Michael R, Alter, Leila A, Pekar, Allen H, Havel, Henry A, Brems, David N

“…It has been previously shown, by equilibrium denaturation, that human growth hormone (hGH) folds by a cooperative two-state process. This is in contrast to the…”
Get full text

Acid stabilization of insulin by Bryant, Christopher, Spencer, Donald B, Miller, Alita, Bakaysa, Diane L, McCune, Karen S, Maple, Steven R, Pekar, Allen H, Brems, David N

“…The effect of pH on the conformational stability of insulin was studied. Surprisingly, the Gibbs free energy of unfolding increased approximately 30% by…”
Get full text

Hypoglycemic potency and metabolic clearance rate of intravenously administered human proinsulin and metabolites by Tillil, H, Frank, B H, Pekar, A H, Broelsch, C, Rubenstein, A H, Polonsky, K S

“…Since circulating proinsulin has been suggested to be important in the pathogenesis of noninsulin-dependent diabetes, and biosynthetic human proinsulin (HPI)…”
Get more information

Detection of an equilibrium intermediate in the folding of a monomeric insulin analog by Bryant, Christopher, Strohl, Margaret, Green, L. Kenney, Long, Harlan B, Alter, Leila A, Pekar, Alan H, Chance, Ronald E, Brems, David N

“…To determine the conformational properties of the C-terminal region of the insulin B-chain relative to the helical core of the molecule, we have investigated…”
Get full text

Physicochemical basis for the rapid time‐action of LysB28ProB29‐insulin: Dissociation of a protein‐ligand complex by Bakaysa, Diane L., Radziuk, Jerry, Havel, Henry A., Brader, Mark L., Li, Shun, Dodd, Steven W., Beals, John M., Pekar, Allen H., Brems, David N.

“…The rate‐limiting step for the absorption of insulin solutions after subcutaneous injection is considered to be the dissociation of self‐associated hexamers to…”
Get full text

Effect of salts on the structure of a potent analog of growth hormone releasing hormone as determined by optical spectroscopy by Needham, G F, Pekar, A H, Havel, H A

“…Optical spectroscopic methods (circular dichroism, analytical ultracentrifugation, and static light scattering) were employed to study the solution behavior of…”
Get more information

Conformation of proinsulin. Comparison of insulin and proinsulin self-association at neutral pH by Pekar, Allen H, Frank, Bruce H

Get full text

Physical Properties of Nitroglucagons and Aminoglucagons by Frank, Bruce H., Pekar, Allen H.

“…Mononitroglucagon (NO2 group on tyrosine residue 13) exhibits physical properties very similar to those of unmodified glucagon. The far ultraviolet CD spectra…”
Get full text

Physical studies on proinsulin. Comparison of the titration behavior of the tyrosine residues in insulin and proinsulin by Frank, B. H, Veros, A. J, Pekar, A. H

Get full text

Isolation and characterization of a genetic variant of bovine proinsulin by Frank, B H, Pekar, A H, Pettee, J M, Schirmer, E M, Johnson, M G, Chance, R E

“…A genetic variant of bovine proinsulin has been isolated using preparative reverse-phase HPLC. The new proinsulin (bovine proinsulin II) differs from the known…”
Get more information

Insulin and proinsulin conformation in solution by Frank, B H, Pekar, A H, Veros, A J

“…The physical chemistry of the insulin molecule in solution is reviewed. The nature of the self-association behavior is such that at physiological concentration…”
Get full text

Crystalline l-Asparaginase from Escherichia coli B by Frank, Bruce H., Pekar, Allen H., Veros, Angelo J., Ho, Peter P.K.

“…Crystalline l -asparaginase from Escherichia coli B has been shown to consist of four subunits (mol wt 33,000) by the methods of sedimentation velocity and…”
Get full text

Glucagon Structure and Function by Bromer, William W., Boucher, Melinda E., Patterson, Jefferson M., Pekar, Allen H., Frank, Bruce H.

“…Crystalline bovine or porcine glucagon was fractionated by chromatography on columns of DEAE-cellulose in the presence of buffers containing 7 m urea. About…”
Get full text