Search Results - "Pei, Jinfeng"
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Bridging PCR: An Efficient and Reliable Scheme Implemented for Genome-Walking
Published in Current issues in molecular biology (05-01-2023)“…The efficacy of the available genome-walking methods is restricted by low specificity, high background, or composite operations. We herein conceived bridging…”
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Semi-Site-Specific Primer PCR: A Simple but Reliable Genome-Walking Tool
Published in Current issues in molecular biology (05-01-2023)“…Genome-walking has been frequently applied to molecular biology and related areas. Herein, a simple but reliable genome-walking technique, termed…”
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Fusion primer driven racket PCR: A novel tool for genome walking
Published in Frontiers in genetics (18-10-2022)“…The limitations of the current genome-walking strategies include strong background and cumbersome experimental processes. Herein, we report a genome-walking…”
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DAR-PCR: a new tool for efficient retrieval of unknown flanking genomic DNA
Published in AMB Express (12-10-2022)“…Various PCR-based genome-walking methods have been developed to acquire unknown flanking DNA sequences. However, the specificity and efficacy levels, and the…”
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Production of Gamma-Aminobutyric Acid by Levilactobacillus brevis CD0817 by Coupling Fermentation with Self-Buffered Whole-Cell Catalysis
Published in Fermentation (Basel) (01-07-2022)“…There is a recent trend of using lactic acid bacteria for the production of gamma-aminobutyric acid (GABA). This study described a method that combines…”
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Wristwatch PCR: A Versatile and Efficient Genome Walking Strategy
Published in Frontiers in bioengineering and biotechnology (12-04-2022)“…Genome walking is a method used to retrieve unknown flanking DNA. Here, we reported wristwatch (WW) PCR, an efficient genome walking technique mediated by WW…”
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Protocol to access unknown flanking DNA sequences using Wristwatch-PCR for genome-walking
Published in STAR protocols (17-03-2023)“…Here we describe a protocol for wristwatch PCR, an approach based on wristwatch-like structure formed between walking primers to obtain unknown flanks. We…”
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Sodium-Ion-Free Fermentative Production of GABA with Levilactobacillus brevis CD0817
Published in Metabolites (28-04-2023)“…Gamma-aminobutyric acid (GABA) has positive effects on many physiological processes. Lactic acid bacterial production of GABA is a future trend. This study…”
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Preparation and characterization of cysteine-rich collagen peptide and its antagonistic effect on microplastic induced damage to HK-2 cells
Published in Food bioscience (01-10-2024)“…Cysteine (Cys) plays a significant role in the stability and antioxidant capacity of peptide. This study was to prepare Cys-rich collagen peptide and evaluate…”
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Sodium-Ion-Free Fermentative Production of GABA with ILevilactobacillus brevis/I CD0817
Published in Metabolites (01-04-2023)“…Gamma-aminobutyric acid (GABA) has positive effects on many physiological processes. Lactic acid bacterial production of GABA is a future trend. This study…”
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