In vitro propagation of almond (Prunus dulcis) cv. Merced

In vitro propagation of almond (Prunus dulcis) cv. Merced

Forced and unforced shoot tips were surface sterilized with different sterilant regimes and incubated under normal culture room conditions. Surface sterilization of explants with mercuric chloride 0.1% (w/v) for 10 min. was found effective in improving culture asepsis (51.66%) and explant survival (...

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Bibliographic Details
Published in:The Indian journal of agricultural sciences Vol. 82; no. 4; pp. 312 - 7
Main Authors: Mir, M.A, Bhat, K.M, Rather, Z.A, Peer, F.A, Pandit, A.H, Hussain, G
Format: Journal Article
Language:English
Published: 01-04-2012
Subjects:
ALMENDRA
ALMONDS
AMANDE
BOUTURE
CONTROL DEL CRECIMIENTO
CONTROLE DE CROISSANCE
CUTTINGS
ENRACINEMENT
ENRAIZAMIENTO
ESQUEJES
GROWTH CONTROL
http://www.fao.org/aos/agrovoc#c_15980
http://www.fao.org/aos/agrovoc#c_2048
http://www.fao.org/aos/agrovoc#c_24136
http://www.fao.org/aos/agrovoc#c_37411
http://www.fao.org/aos/agrovoc#c_37563
http://www.fao.org/aos/agrovoc#c_6649
http://www.fao.org/aos/agrovoc#c_8734
IN VITRO
MICROPROPAGACION
MICROPROPAGATION
PROLIFERACION DE TEJIDOS
PROLIFERATION DES TISSUS
ROOTING
TISSUE PROLIFERATION
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Summary:Forced and unforced shoot tips were surface sterilized with different sterilant regimes and incubated under normal culture room conditions. Surface sterilization of explants with mercuric chloride 0.1% (w/v) for 10 min. was found effective in improving culture asepsis (51.66%) and explant survival (55.00%). Higher values for both these parameters were recorded with forced explants in comparison to unforced ones. Main effect of growth regulators and media was significant on explant establishment which was maximum (66.66%) on ½ MS media containing BAP + IBA (0.50+0.01 mg/l). Callusing at the base of initiating cultures was minimum (24.58%) with BAP+IBA (0.25+0.01 mg/l). Microshoots from the established cultures were subcultured on the MS media supplemented with BAP and NAA alone or in combination for axillary shoot proliferation. Maximum proliferated cultures (86.66%) with maximum shoot number/explant (15.61) and proliferation grade (4.00) was obtained with BAP+NAA ( 0.40 + 0.01 mg/l). BAP was found superior to NAA during axillary shoot proliferation. Microshoots (10_15 mm) from proliferated cultures were subcultured in root induction medium (MS medium supplemented with IBA) and incubated under darkness for 10 days at 24±1 degree Celsius and then transferred to root development medium (hormone-free MS medium) and incubated under normal culture room conditions. Highest rooting of microshoots (93.33%) with maximum root number/shoot (5.90) and root length (43.00 mm) was obtained with IBA (1.0 mg/l).
Bibliography:2012000145
http://epubs.icar.org.in/ejournal/index.php/IJAgS/issue/view/536
F30
ISSN:0019-5022
2394-3319
DOI:10.56093/ijas.v82i4.16632