Variable number of tandem aminoacid repeats in adhesion-related CDS products in Mycoplasma hyopneumoniae strains

The Mycoplasma hyopneumoniae genome contains at least 22 regions with a variable number of tandem nucleotide repeats (VNTRs) within coding DNA sequences (CDSs). In this work, the VNTR-containing CDSs were analysed in order to evaluate their degree of variation, possible correlations with antigenic p...

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Published in:	Veterinary microbiology Vol. 116; no. 4; pp. 258 - 269
Main Authors:	Castro, Luiza Amaral de, Pedroso, Thiago Rodrigues, Kuchiishi, Suzana Satomi, Ramenzoni, Marni, Kich, Jalusa Deon, Zaha, Arnaldo, Vainstein, Marilene Henning, Ferreira, Henrique Bunselmeyer
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 10-09-2006 Elsevier Science
Subjects:	Adhesins Adhesins, Bacterial - chemistry Adhesins, Bacterial - genetics Amino Acid Sequence amino acid sequences Aminoacid repeats Animals antigenic variation Antigens, Bacterial - chemistry Antigens, Bacterial - genetics Antigens, Bacterial - immunology bacterial adhesion Bacterial Adhesion - genetics Bacterial Outer Membrane Proteins - chemistry Bacterial Outer Membrane Proteins - genetics Bacterial Outer Membrane Proteins - immunology bacterial pneumonia Bacteriology Base Sequence Biological and medical sciences Blotting, Southern DNA, Bacterial - analysis DNA, Bacterial - chemistry Enzootic pneumonia Fundamental and applied biological sciences. Psychology Genes, Bacterial genetic techniques and protocols Genetic Variation genome Interstrain variability Microbiology minisatellite repeats Miscellaneous molecular genetics Molecular Sequence Data Mycoplasma hyopneumoniae Mycoplasma hyopneumoniae - genetics Mycoplasma hyopneumoniae - pathogenicity mycoplasmosis pathogenicity Pneumonia of Swine, Mycoplasmal - microbiology polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary respiratory tract diseases Sequence Alignment - veterinary sequence analysis Sequence Analysis, DNA strain differences Strain typing strains Swine swine diseases swine enzootic pneumonia Tandem Repeat Sequences - genetics Tandem Repeat Sequences - immunology VNTR Aminoacid repeats Adhesins Interstrain variability Strain typing VNTR Enzootic pneumonia Lung disease Pneumonia Typing Microbiology Respiratory disease Adhesion Mycoplasmataceae Strain Mollicutes Mycoplasmatales Mycoplasma hyopneumoniae Minisatellite DNA Aminoacid Bacteria Adhesin Veterinary
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Summary:	The Mycoplasma hyopneumoniae genome contains at least 22 regions with a variable number of tandem nucleotide repeats (VNTRs) within coding DNA sequences (CDSs). In this work, the VNTR-containing CDSs were analysed in order to evaluate their degree of variation, possible correlations with antigenic properties, and their potential to be used as a basis for a strain typing PCR assay. We have analysed the VNTRs in five M. hyopneumoniae strains (J, 7448, 7422, PMS, and 232), based on published genomic sequences and on amplified and sequenced DNA segments. These VNTRs are distributed among 12 genes, most of which encode putative surface proteins, including known adhesins. The number of repeat units in any of the VNTRs is highly variable among the analysed strains, but they are, without exception, translationally in frame, and, therefore, code for a variable number of aminoacid repeats (VNTARs). These VNTARs determine putative structural, physicochemical and antigenic variations in the corresponding proteins, with potential implications for aspects associated to M. hyopneumoniae pathogenicity, such as cell adhesion and interactions with the host immune system. Considering that the characterized VNTARs are relatively stable, at least in vitro, and their sizes are strain-specific, we have developed a VNTR-based PCR assay for M. hyopneumoniae strain identification, useful for enzootic pneumonia (EP) diagnosis, strain typing, and distinction of circulating field isolates from vaccine strains in animals vaccinated against EP.
Bibliography:	http://dx.doi.org/10.1016/j.vetmic.2006.04.022 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0378-1135 1873-2542
DOI:	10.1016/j.vetmic.2006.04.022