Ultrasound‐Assisted Enzyme‐Catalyzed Hydrolysis of Collagen to Produce Peptides With Biomedical Potential: Collagenase From Aspergillus terreus UCP1276

Ultrasound‐Assisted Enzyme‐Catalyzed Hydrolysis of Collagen to Produce Peptides With Biomedical Potential: Collagenase From Aspergillus terreus UCP1276

Ultrasound has been applied for varied purposes as it provides additional mechanical energy to a system, and is still profitable and straightforward, which are advantages for industrial applications. In this work, ultrasonic treatments were applied to purified collagenase fractions from a fermented...

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Published in:Bioelectromagnetics Vol. 41; no. 2; pp. 113 - 120
Main Authors: Costa‐Junior, Romualdo B., Brandão‐Costa, Romero M. P., Albuquerque, Wendell W. C., Batista, Juanize M. S., Pedrosa, Raquel B., Porto, Ana L. F.
Format: Journal Article
Language:English
Published: United States Wiley Subscription Services, Inc 01-02-2020
Subjects:
anticoagulant
Anticoagulants
Aspergillus terreus
biocatalytic activity
Biological activity
Collagen
Collagenase
Electrophoresis
Enzymes
Fermented food
Fluorides
Gel electrophoresis
Hydrolysis
Industrial applications
Peptides
Polyacrylamide
proteases
Prothrombin
Sodium dodecyl sulfate
Sodium lauryl sulfate
Ultrasonic imaging
Ultrasonic testing
Ultrasound
ultrasound pretreatment
collagenase
anticoagulant
ultrasound pretreatment
proteases
biocatalytic activity
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Summary:Ultrasound has been applied for varied purposes as it provides additional mechanical energy to a system, and is still profitable and straightforward, which are advantages for industrial applications. In this work, ultrasonic treatments were applied to purified collagenase fractions from a fermented extract by Aspergillus terreus UCP 1276 aiming to evaluate the potential effect on collagen hydrolysis. The physical agent was evaluated as an inductor of collagen degradation and consequently as a producer of peptides with anticoagulant activity. The sodium dodecyl sulphate‐polyacrylamide gel electrophoresis analyses were also carried out to compare the hydrolysis techniques. The ultrasound (40 kHz, 47.4 W/L) processing was conducted under the same conditions of pH and temperature at different times. The ultrasound‐assisted reaction was accelerated in relation to conventional processing. Collagenolytic activity was enhanced and tested in the presence of phenylmethanesulfonyl fluoride inhibitor. Underexposure, the activity was enhanced, reaching more than 72.0% of improvement in relation to the non‐exposed enzyme. A period of 30 min of incubation under ultrasound exposure was enough to efficiently produce peptides with biological activity, including anticoagulation and effect on prothrombin time at about 60%. The results indicate that low‐frequency ultrasound is an enzymatic inducer with likely commercial applicability accelerating the enzymatic reaction. Bioelectromagnetics. 2020;41:113–120. © 2019 Bioelectromagnetics Society.
Bibliography:Conflict of interest: None.
Grant sponsor: FACEPE (Foundation for Science and Technology of the State of Pernambuco); grant number: APQ. 1200‐2.12/15; Grant sponsor: CNPq (National Council for Scientific and Technological Development), grant number: 402529/2012‐0.
ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0197-8462
1521-186X
DOI:10.1002/bem.22239