Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil

BACKGROUNDDespite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear. METHODSWe evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some of t...

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Published in:	Revista da Sociedade Brasileira de Medicina Tropical Vol. 55; p. e0067
Main Authors:	Coletti, Thaís de Moura, Romano, Camila Malta, Urbinatti, Paulo Roberto, Teixeira, Renildo Souza, Pedrosa, Leila Weiss de Almeida, Nardi, Marcello Schiavo, Natal, Delsio, Costa, Antônio Charlys da, Jesus, Jaqueline Goes de, Claro, Ingra Morales, Sabino, Ester Cerdeira, Witkin, Steven S., Marrelli, Mauro Toledo, Fernandes, Licia Natal
Format:	Journal Article
Language:	English
Published:	Sociedade Brasileira de Medicina Tropical - SBMT 01-01-2022 Sociedade Brasileira de Medicina Tropical (SBMT)
Subjects:	Culex flavivirus Insect-specific flavivirus Mosquito Short Communication TROPICAL MEDICINE Urban park Urban park Culex flavivirus Mosquito Insect-specific flavivirus
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Summary:	BACKGROUNDDespite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear. METHODSWe evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some of the positive samples were subjected to nanopore sequencing. RESULTSTwelve mosquito pools (2.8%) tested positive for flavivirus infection. Nanopore sequencing was successfully performed on six samples. Phylogenetic analysis grouped these sequences into genotype 2 of Culex flavivirus (CxFV). CONCLUSIONSThe identification of CxFV genotype 2 at new locations in São Paulo highlights the importance of understanding the role of ISFs in mosquito vector competence.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Authors’ contribution: DN, MTM, LNF: Conceptualization; ECS, MTM, LNF: Funding acquisition; TMC, PRU, RST, MSN, DN, ACC, JGJ, IMC, MTM, LNF: Investigation; TMC, PRU, RST, MSN, DN, ACC, JGJ, IMC, ECS, MTM, LNF: Methodology; MTM, LNF: Project administration; CMR, RST, MSN, PRU, DN, ACC, ECS, MTM, LNF: Resources; CMR, ACC, JGJ, IMC, SSW, MTM, LNF: Supervision; TMC, CMR, ACC, IMC, SSW, MTM, LNF: Writing original draft, review & editing. Conflict of Interest: The authors declare that there is no conflict of interest.
ISSN:	0037-8682 1678-9849 1678-9849
DOI:	10.1590/0037-8682-0067-2022