Lipase-mediated hydrolysis of hempseed oil in a packed-bed reactor and in-line purification of PUFA as mono- and diacylglycerols

[Display omitted] •A lipase-catalyzed hydrolysis of hempseed oil was performed.•A packed-bed reactor (PBR) coupled with an in-line purification apparatus was used.•The highest hydrolysis degree (60%) was achieved at a flow rate of 0.092mL/min.•The glyceride fraction was obtained in 40% yield in less...

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Bibliographic Details
Published in:Food and bioproducts processing Vol. 123; pp. 345 - 353
Main Authors: Bavaro, T., Benucci, I., Pedrali, A., Marrubini, G., Esti, M., Terreni, M., Massolini, G., Ubiali, D.
Format: Journal Article
Language:English
Published: Rugby Elsevier B.V 01-09-2020
Elsevier Science Ltd
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Summary:[Display omitted] •A lipase-catalyzed hydrolysis of hempseed oil was performed.•A packed-bed reactor (PBR) coupled with an in-line purification apparatus was used.•The highest hydrolysis degree (60%) was achieved at a flow rate of 0.092mL/min.•The glyceride fraction was obtained in 40% yield in less than 2h.•Mono- and diacylglycerols were composed of linoleic, α-linolenic, and oleic acid. Mono- and diacylglycerols (MAG and DAG, respectively) of polyunsaturated fatty acids (PUFA) are raising an increasing interest as novel non-ionic food emulsifiers owing to the recognized health-promoting effects of PUFA. We here reported on the lipase-catalyzed hydrolysis of hempseed oil in a homogeneous medium based on oil and t-BuOH/water carried out in a continuous flow packed-bed reactor (PBR). The biotransformation was carried out by feeding the reaction mixture upwards the PBR at varying flow rates through a peristaltic pump. The system was coupled in-line with a second glass column filled with an ion-exchange resin to retain hydrolyzed free fatty acids (FFA), thus allowing the straightforward purification of the glyceride component. A mixture of MAG and DAG (yield 40%, 620mg) composed of linoleic and α-linolenic acid (82%) and oleic acid (16%) was obtained in less than 2h. The lipase-based PBR was used for 10 cycles without any loss of activity.
ISSN:0960-3085
1744-3571
DOI:10.1016/j.fbp.2020.07.009