Application of Measurements of Transepithelial Electrical Resistance of Intestinal Epithelial Cell Monolayers To Evaluate Probiotic Activity

Application of Measurements of Transepithelial Electrical Resistance of Intestinal Epithelial Cell Monolayers To Evaluate Probiotic Activity

Among five potentially probiotic lactobacilli investigated, Lactobacillus plantarum MF1298 and Lactobacillus salivarius DC5 showed the highest increase in the transepithelial electrical resistance (TER) of polarized monolayers of Caco-2 cells, and this increase was shown to be dose dependent. Furthe...

Full description

Saved in:
Bibliographic Details
Published in:Applied and Environmental Microbiology Vol. 71; no. 11; pp. 7528 - 7530
Main Authors: Klingberg, Trine Danø, Pedersen, Maja Herold, Cencic, Avrelija, Budde, Birgitte Bjørn
Format: Journal Article
Language:English
Published: Washington, DC American Society for Microbiology 01-11-2005
Subjects:
Bacteria
Biological and medical sciences
Caco-2 Cells
cell lines
Cell Polarity
Electric Impedance
electrical resistance
Epithelial Cells - microbiology
Epithelial Cells - physiology
Fundamental and applied biological sciences. Psychology
Genes
Humans
intestinal mucosa
Intestines - cytology
Intestines - microbiology
Lactobacillus
Lactobacillus - growth & development
Lactobacillus - physiology
Lactobacillus plantarum
Lactobacillus salivarius
Listeria monocytogenes
Listeria monocytogenes - pathogenicity
membrane permeability
Microbial Ecology
Microbiology
pathogenicity
probiotic activity
probiotics
Probiotics - metabolism
Small intestine
transepithelial electrical resistance
Probiotic
Electric resistivity
Monolayer
Epithelial cell
Application
Gut
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Among five potentially probiotic lactobacilli investigated, Lactobacillus plantarum MF1298 and Lactobacillus salivarius DC5 showed the highest increase in the transepithelial electrical resistance (TER) of polarized monolayers of Caco-2 cells, and this increase was shown to be dose dependent. Furthermore, preincubation with MF1298 attenuated a decrease in TER induced by Listeria monocytogenes.
Bibliography:http://aem.asm.org/contents-by-date.0.shtml
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Corresponding author. Mailing address: The Royal Veterinary and Agricultural University, Department of Food Science, Rolighedsvej 30, 4th Floor, DK-1958 Frederiksberg C, Denmark. Phone: 45 35 28 33 26. Fax: 45 35 28 32 14. E-mail: tdk@kvl.dk.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.71.11.7528-7530.2005